Characterizing lung metabolism with carbon-13 magnetic resonance spectroscopy in a small-animal model : Evidence of gluconeogenesis during hypothermic storage
Experimental evidence suggests storing lungs inflated with oxygen and with oxidizable substrate improves results of lung transplantation. Glucose is included in the low-potassium-dextran (LPD) solution Perfadex to achieve this goal. The authors hypothesized that other substrates might be more effect...
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| Veröffentlicht in: | Transplantation 2005-08, Vol.80 (3), p.417-420 |
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| creator | PELTZ, Matthias HE, Tian-Teng ADAMS, Glenn A CHAO, Robert Y MEYER, Dan M JESSEN, Michael E |
| description | Experimental evidence suggests storing lungs inflated with oxygen and with oxidizable substrate improves results of lung transplantation. Glucose is included in the low-potassium-dextran (LPD) solution Perfadex to achieve this goal. The authors hypothesized that other substrates might be more effective. Rat lungs were stored for 6 or 24 hr in LPD solution with the following carbon-13--labeled substrates: 5 mM glucose (Perfadex group), 32 mM pyruvate (pyruvate group), or both (combination group). Metabolism was assessed by magnetic resonance spectroscopy. Small amounts of exogenous glucose were oxidized in the Perfadex group. In contrast, exogenous pyruvate was the major substrate oxidized in the pyruvate and combination groups (P |
| doi_str_mv | 10.1097/01.tp.0000169129.45433.b6 |
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Glucose is included in the low-potassium-dextran (LPD) solution Perfadex to achieve this goal. The authors hypothesized that other substrates might be more effective. Rat lungs were stored for 6 or 24 hr in LPD solution with the following carbon-13--labeled substrates: 5 mM glucose (Perfadex group), 32 mM pyruvate (pyruvate group), or both (combination group). Metabolism was assessed by magnetic resonance spectroscopy. Small amounts of exogenous glucose were oxidized in the Perfadex group. In contrast, exogenous pyruvate was the major substrate oxidized in the pyruvate and combination groups (P<0.01 vs. Perfadex). Carbon-13--labeled glucose and glycogen were detected in the pyruvate group, suggesting that gluconeogenesis and glycogen synthesis occur in glucose-deprived lungs. Lungs for transplantation metabolize substrates through both anabolic and catabolic pathways. These reactions may be important in designing improved solutions for lung preservation.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/01.tp.0000169129.45433.b6</identifier><identifier>PMID: 16082340</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Animals ; Biological and medical sciences ; Carbon Isotopes - metabolism ; Dextrans - pharmacology ; Disease Models, Animal ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gluconeogenesis ; Glucose - metabolism ; Glycogen - chemistry ; Hypothermia, Induced ; Lung - metabolism ; Lung - pathology ; Lung Transplantation - methods ; Magnetic Resonance Spectroscopy - methods ; Male ; Medical sciences ; Models, Animal ; Organ Preservation - methods ; Organ Preservation Solutions - pharmacology ; Oxygen - metabolism ; Potassium - pharmacology ; Pyruvates - metabolism ; Pyruvic Acid - pharmacology ; Rats ; Rats, Sprague-Dawley ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Time Factors ; Tissue, organ and graft immunology</subject><ispartof>Transplantation, 2005-08, Vol.80 (3), p.417-420</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-ad8d5659941bee2388205533e2b86fd5651eb0f72da26d7f75676b1ae308b6b93</citedby><cites>FETCH-LOGICAL-c427t-ad8d5659941bee2388205533e2b86fd5651eb0f72da26d7f75676b1ae308b6b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17051373$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16082340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PELTZ, Matthias</creatorcontrib><creatorcontrib>HE, Tian-Teng</creatorcontrib><creatorcontrib>ADAMS, Glenn A</creatorcontrib><creatorcontrib>CHAO, Robert Y</creatorcontrib><creatorcontrib>MEYER, Dan M</creatorcontrib><creatorcontrib>JESSEN, Michael E</creatorcontrib><title>Characterizing lung metabolism with carbon-13 magnetic resonance spectroscopy in a small-animal model : Evidence of gluconeogenesis during hypothermic storage</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Experimental evidence suggests storing lungs inflated with oxygen and with oxidizable substrate improves results of lung transplantation. Glucose is included in the low-potassium-dextran (LPD) solution Perfadex to achieve this goal. The authors hypothesized that other substrates might be more effective. Rat lungs were stored for 6 or 24 hr in LPD solution with the following carbon-13--labeled substrates: 5 mM glucose (Perfadex group), 32 mM pyruvate (pyruvate group), or both (combination group). Metabolism was assessed by magnetic resonance spectroscopy. Small amounts of exogenous glucose were oxidized in the Perfadex group. In contrast, exogenous pyruvate was the major substrate oxidized in the pyruvate and combination groups (P<0.01 vs. Perfadex). Carbon-13--labeled glucose and glycogen were detected in the pyruvate group, suggesting that gluconeogenesis and glycogen synthesis occur in glucose-deprived lungs. Lungs for transplantation metabolize substrates through both anabolic and catabolic pathways. These reactions may be important in designing improved solutions for lung preservation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbon Isotopes - metabolism</subject><subject>Dextrans - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gluconeogenesis</subject><subject>Glucose - metabolism</subject><subject>Glycogen - chemistry</subject><subject>Hypothermia, Induced</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>Lung Transplantation - methods</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Models, Animal</subject><subject>Organ Preservation - methods</subject><subject>Organ Preservation Solutions - pharmacology</subject><subject>Oxygen - metabolism</subject><subject>Potassium - pharmacology</subject><subject>Pyruvates - metabolism</subject><subject>Pyruvic Acid - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Time Factors</subject><subject>Tissue, organ and graft immunology</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-u1SAQxonReI9XX8HgQnetUAq07szJ9U9yEze6JkCnPRhaKlDN8WF8Vqn3JGfpLJgFv_m-yXwIvaKkpqSXbwmt81qTUlT0tOnrlreM1UY8QgfKWVsJ0pHH6EBISyvKmLxBz1L6XnjOpHyKbmgBGtaSA_pzPOmobYbofrtlwn4rzwxZm-BdmvEvl0_Y6mjCUpTwrKcFsrM4QgqLXizgtILNMSQb1jN2C9Y4zdr7Si-udDyHATx-h-9-ugF2Pox48psNC4QJFkgu4WGLu_fpvIZ8gjgX_ZRD1BM8R09G7RO8uPRb9O3D3dfjp-r-y8fPx_f3lW0bmSs9dAMXvO9bagAa1nUN4ZwxaEwnxv2LgiGjbAbdiEGOkgspDNXASGeE6dktevOgu8bwY4OU1eySBe91WXNLSnQtZ6IT_wWp5FI0jBawfwBtuU2KMKo1loPEs6JE7SkqQlVe1TVF9S9FZXaTlxeTzcwwXCcvsRXg9QXQyWo_xpKES1dOEk6ZZOwvC5OpdA</recordid><startdate>20050815</startdate><enddate>20050815</enddate><creator>PELTZ, Matthias</creator><creator>HE, Tian-Teng</creator><creator>ADAMS, Glenn A</creator><creator>CHAO, Robert Y</creator><creator>MEYER, Dan M</creator><creator>JESSEN, Michael E</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050815</creationdate><title>Characterizing lung metabolism with carbon-13 magnetic resonance spectroscopy in a small-animal model : Evidence of gluconeogenesis during hypothermic storage</title><author>PELTZ, Matthias ; HE, Tian-Teng ; ADAMS, Glenn A ; CHAO, Robert Y ; MEYER, Dan M ; JESSEN, Michael E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-ad8d5659941bee2388205533e2b86fd5651eb0f72da26d7f75676b1ae308b6b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbon Isotopes - metabolism</topic><topic>Dextrans - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gluconeogenesis</topic><topic>Glucose - metabolism</topic><topic>Glycogen - chemistry</topic><topic>Hypothermia, Induced</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>Lung Transplantation - methods</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Models, Animal</topic><topic>Organ Preservation - methods</topic><topic>Organ Preservation Solutions - pharmacology</topic><topic>Oxygen - metabolism</topic><topic>Potassium - pharmacology</topic><topic>Pyruvates - metabolism</topic><topic>Pyruvic Acid - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Time Factors</topic><topic>Tissue, organ and graft immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PELTZ, Matthias</creatorcontrib><creatorcontrib>HE, Tian-Teng</creatorcontrib><creatorcontrib>ADAMS, Glenn A</creatorcontrib><creatorcontrib>CHAO, Robert Y</creatorcontrib><creatorcontrib>MEYER, Dan M</creatorcontrib><creatorcontrib>JESSEN, Michael E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PELTZ, Matthias</au><au>HE, Tian-Teng</au><au>ADAMS, Glenn A</au><au>CHAO, Robert Y</au><au>MEYER, Dan M</au><au>JESSEN, Michael E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterizing lung metabolism with carbon-13 magnetic resonance spectroscopy in a small-animal model : Evidence of gluconeogenesis during hypothermic storage</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2005-08-15</date><risdate>2005</risdate><volume>80</volume><issue>3</issue><spage>417</spage><epage>420</epage><pages>417-420</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Experimental evidence suggests storing lungs inflated with oxygen and with oxidizable substrate improves results of lung transplantation. Glucose is included in the low-potassium-dextran (LPD) solution Perfadex to achieve this goal. The authors hypothesized that other substrates might be more effective. Rat lungs were stored for 6 or 24 hr in LPD solution with the following carbon-13--labeled substrates: 5 mM glucose (Perfadex group), 32 mM pyruvate (pyruvate group), or both (combination group). Metabolism was assessed by magnetic resonance spectroscopy. Small amounts of exogenous glucose were oxidized in the Perfadex group. In contrast, exogenous pyruvate was the major substrate oxidized in the pyruvate and combination groups (P<0.01 vs. Perfadex). Carbon-13--labeled glucose and glycogen were detected in the pyruvate group, suggesting that gluconeogenesis and glycogen synthesis occur in glucose-deprived lungs. Lungs for transplantation metabolize substrates through both anabolic and catabolic pathways. 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| subjects | Animals Biological and medical sciences Carbon Isotopes - metabolism Dextrans - pharmacology Disease Models, Animal Fundamental and applied biological sciences. Psychology Fundamental immunology Gluconeogenesis Glucose - metabolism Glycogen - chemistry Hypothermia, Induced Lung - metabolism Lung - pathology Lung Transplantation - methods Magnetic Resonance Spectroscopy - methods Male Medical sciences Models, Animal Organ Preservation - methods Organ Preservation Solutions - pharmacology Oxygen - metabolism Potassium - pharmacology Pyruvates - metabolism Pyruvic Acid - pharmacology Rats Rats, Sprague-Dawley Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Time Factors Tissue, organ and graft immunology |
| title | Characterizing lung metabolism with carbon-13 magnetic resonance spectroscopy in a small-animal model : Evidence of gluconeogenesis during hypothermic storage |
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