Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry
Mass spectrometric analysis of biomolecules under ambient conditions promises to enable the in vivo investigation of diverse biochemical changes in organisms with high specificity. Here we report on a novel combination of infrared laser ablation with electrospray ionization (LAESI) as an ambient ion...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2007-11, Vol.79 (21), p.8098-8106 |
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| creator | Nemes, Peter Vertes, Akos |
| description | Mass spectrometric analysis of biomolecules under ambient conditions promises to enable the in vivo investigation of diverse biochemical changes in organisms with high specificity. Here we report on a novel combination of infrared laser ablation with electrospray ionization (LAESI) as an ambient ion source for mass spectrometry. As a result of the interactions between the ablation plume and the spray, LAESI accomplishes electrospray-like ionization. Without any sample preparation or pretreatment, this technique was capable of detecting a variety of molecular classes and size ranges (up to 66 kDa) with a detection limit of 8 and 25 fmol for verapamil and reserpine, respectively, and quantitation capabilities with a four-decade dynamic range. We demonstrated the utility of LAESI in a broad variety of applications ranging from plant biology to clinical analysis. Proteins, lipids, and metabolites were identified, and antihistamine excretion was followed via the direct analysis of bodily fluids (urine, blood, and serum). We also performed in vivo spatial profiling (on leaf, stem, and root) of metabolites in a French marigold (Tagetes patula) seedling. |
| doi_str_mv | 10.1021/ac071181r |
| format | Article |
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Here we report on a novel combination of infrared laser ablation with electrospray ionization (LAESI) as an ambient ion source for mass spectrometry. As a result of the interactions between the ablation plume and the spray, LAESI accomplishes electrospray-like ionization. Without any sample preparation or pretreatment, this technique was capable of detecting a variety of molecular classes and size ranges (up to 66 kDa) with a detection limit of 8 and 25 fmol for verapamil and reserpine, respectively, and quantitation capabilities with a four-decade dynamic range. We demonstrated the utility of LAESI in a broad variety of applications ranging from plant biology to clinical analysis. Proteins, lipids, and metabolites were identified, and antihistamine excretion was followed via the direct analysis of bodily fluids (urine, blood, and serum). 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Chem</addtitle><description>Mass spectrometric analysis of biomolecules under ambient conditions promises to enable the in vivo investigation of diverse biochemical changes in organisms with high specificity. Here we report on a novel combination of infrared laser ablation with electrospray ionization (LAESI) as an ambient ion source for mass spectrometry. As a result of the interactions between the ablation plume and the spray, LAESI accomplishes electrospray-like ionization. Without any sample preparation or pretreatment, this technique was capable of detecting a variety of molecular classes and size ranges (up to 66 kDa) with a detection limit of 8 and 25 fmol for verapamil and reserpine, respectively, and quantitation capabilities with a four-decade dynamic range. We demonstrated the utility of LAESI in a broad variety of applications ranging from plant biology to clinical analysis. Proteins, lipids, and metabolites were identified, and antihistamine excretion was followed via the direct analysis of bodily fluids (urine, blood, and serum). We also performed in vivo spatial profiling (on leaf, stem, and root) of metabolites in a French marigold (Tagetes patula) seedling.</description><subject>Analytical chemistry</subject><subject>Atmospheric Pressure</subject><subject>Body fluids</subject><subject>Humans</subject><subject>Ions</subject><subject>Mass spectrometry</subject><subject>Molecular Weight</subject><subject>Organisms</subject><subject>Particle Size</subject><subject>Plant Leaves - chemistry</subject><subject>Plant Roots - chemistry</subject><subject>Plant Stems - chemistry</subject><subject>Proteins - analysis</subject><subject>Reserpine - analysis</subject><subject>Seedlings - chemistry</subject><subject>Seeds</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>Spectrum analysis</subject><subject>Tagetes - chemistry</subject><subject>Terfenadine - administration & dosage</subject><subject>Terfenadine - analogs & derivatives</subject><subject>Terfenadine - analysis</subject><subject>Terfenadine - urine</subject><subject>Verapamil - analysis</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0F1rFDEUBuAgFrtWL_wDEgSFQkdzJl8zl2Wp7cIWi61eeBMymTNr6nysyUzp-uuNztKCXoXkPLw5vIS8AvYeWA4frGMaoIDwhCxA5ixTRZE_JQvGGM9yzdgheR7jLWMADNQzcgi6TBehFmSzthEDPa1aO_qhp2ctujEMcRvsjq6G3v-a35shobFLg-8YvKNXAWOcAp5Q39Ov_m44obav6aqzG99v6KWNkV5v_2Z1OIbdC3LQ2Dbiy_15RL58PLtZXmTrT-er5ek6s0KIMVOyKbWC0lleMdS8KrRgyFGIAmtZW1432gmZN7WARkqXW4VKV1o3loOqJT8i7-bcbRh-ThhH0_nosG1tj8MUjSqE5ErqBN_8A2-HKfRpN5ODLgpVijKh4xm5VEkM2Jht8J0NOwPM_KnePFSf7Ot94FR1WD_KfdcJZDPwccT7h7kNP4zSXEtzc3VtNCuXn8-_gVkm_3b21sXH5f7_-Deddplw</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Nemes, Peter</creator><creator>Vertes, Akos</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry</title><author>Nemes, Peter ; Vertes, Akos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a444t-65f97619ca3b0e73b8740e3e448ed5da3df7c452fd41f55c2a6e67b77fa316d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analytical chemistry</topic><topic>Atmospheric Pressure</topic><topic>Body fluids</topic><topic>Humans</topic><topic>Ions</topic><topic>Mass spectrometry</topic><topic>Molecular Weight</topic><topic>Organisms</topic><topic>Particle Size</topic><topic>Plant Leaves - chemistry</topic><topic>Plant Roots - chemistry</topic><topic>Plant Stems - chemistry</topic><topic>Proteins - analysis</topic><topic>Reserpine - analysis</topic><topic>Seedlings - chemistry</topic><topic>Seeds</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><topic>Spectrum analysis</topic><topic>Tagetes - chemistry</topic><topic>Terfenadine - administration & dosage</topic><topic>Terfenadine - analogs & derivatives</topic><topic>Terfenadine - analysis</topic><topic>Terfenadine - urine</topic><topic>Verapamil - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nemes, Peter</creatorcontrib><creatorcontrib>Vertes, Akos</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nemes, Peter</au><au>Vertes, Akos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>79</volume><issue>21</issue><spage>8098</spage><epage>8106</epage><pages>8098-8106</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Mass spectrometric analysis of biomolecules under ambient conditions promises to enable the in vivo investigation of diverse biochemical changes in organisms with high specificity. Here we report on a novel combination of infrared laser ablation with electrospray ionization (LAESI) as an ambient ion source for mass spectrometry. As a result of the interactions between the ablation plume and the spray, LAESI accomplishes electrospray-like ionization. Without any sample preparation or pretreatment, this technique was capable of detecting a variety of molecular classes and size ranges (up to 66 kDa) with a detection limit of 8 and 25 fmol for verapamil and reserpine, respectively, and quantitation capabilities with a four-decade dynamic range. 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| subjects | Analytical chemistry Atmospheric Pressure Body fluids Humans Ions Mass spectrometry Molecular Weight Organisms Particle Size Plant Leaves - chemistry Plant Roots - chemistry Plant Stems - chemistry Proteins - analysis Reserpine - analysis Seedlings - chemistry Seeds Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization - methods Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis Tagetes - chemistry Terfenadine - administration & dosage Terfenadine - analogs & derivatives Terfenadine - analysis Terfenadine - urine Verapamil - analysis |
| title | Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry |
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