Molecular and biochemical markers for monitoring dynamic shifts of cellulolytic protozoa in Reticulitermes flavipes
Lower termites rely on cellulolytic protozoa to aid in the digestion of their wood-based diet. However, despite the major contribution of protozoa to the lower termite digestive system, few techniques have been developed to monitor shifts in protozoan populations. This study investigated whether qua...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2007-12, Vol.37 (12), p.1366-1374 |
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| creator | Wheeler, Marsha M. Zhou, Xuguo Scharf, Michael E. Oi, Faith M. |
| description | Lower termites rely on cellulolytic protozoa to aid in the digestion of their wood-based diet. However, despite the major contribution of protozoa to the lower termite digestive system, few techniques have been developed to monitor shifts in protozoan populations. This study investigated whether quantitative real-time PCR (qRT-PCR) and/or cellulase enzyme assays can be used to monitor changes of cellulolytic protozoan populations in the lower termite,
Reticulitermes flavipes (Kollar). Previously developed cellulase primer sets were used to test for changes in cellulase gene expression, while three different cellulase enzyme assays were used to assess changes in cellulase enzyme activity. The results from this study indicate that qRT-PCR is a reliable method to monitor shifts in cellulolytic protozoan populations. Specifically, qRT-PCR can serve as a useful monitoring technique during high-throughput screening of novel termite control agents such as cellulase inhibitors, and help to answer questions relating to whether or not such control agents impact cellulolytic protozoan populations. |
| doi_str_mv | 10.1016/j.ibmb.2007.09.010 |
| format | Article |
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Reticulitermes flavipes (Kollar). Previously developed cellulase primer sets were used to test for changes in cellulase gene expression, while three different cellulase enzyme assays were used to assess changes in cellulase enzyme activity. The results from this study indicate that qRT-PCR is a reliable method to monitor shifts in cellulolytic protozoan populations. Specifically, qRT-PCR can serve as a useful monitoring technique during high-throughput screening of novel termite control agents such as cellulase inhibitors, and help to answer questions relating to whether or not such control agents impact cellulolytic protozoan populations.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2007.09.010</identifier><identifier>PMID: 17967355</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biomarkers - metabolism ; Cellulase ; Cellulases - chemistry ; Cellulases - metabolism ; Endoglucanase ; Enzyme Stability ; Eukaryota - enzymology ; Eukaryota - genetics ; Eukaryota - radiation effects ; Exoglucanase ; Isoptera ; Isoptera - genetics ; Isoptera - parasitology ; Isoptera - radiation effects ; Polymerase Chain Reaction ; Protozoa ; Quantitative real-time PCR ; R. flavipes ; Regression Analysis ; Reticulitermes flavipes ; Symbiosis - genetics ; Symbiosis - radiation effects ; Ultraviolet Rays</subject><ispartof>Insect biochemistry and molecular biology, 2007-12, Vol.37 (12), p.1366-1374</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-136a28d7ec866ffb073b4dac8c9b4bd4d8f378678fcdfb194a221fd8d3af35dd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ibmb.2007.09.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17967355$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wheeler, Marsha M.</creatorcontrib><creatorcontrib>Zhou, Xuguo</creatorcontrib><creatorcontrib>Scharf, Michael E.</creatorcontrib><creatorcontrib>Oi, Faith M.</creatorcontrib><title>Molecular and biochemical markers for monitoring dynamic shifts of cellulolytic protozoa in Reticulitermes flavipes</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>Lower termites rely on cellulolytic protozoa to aid in the digestion of their wood-based diet. However, despite the major contribution of protozoa to the lower termite digestive system, few techniques have been developed to monitor shifts in protozoan populations. This study investigated whether quantitative real-time PCR (qRT-PCR) and/or cellulase enzyme assays can be used to monitor changes of cellulolytic protozoan populations in the lower termite,
Reticulitermes flavipes (Kollar). Previously developed cellulase primer sets were used to test for changes in cellulase gene expression, while three different cellulase enzyme assays were used to assess changes in cellulase enzyme activity. The results from this study indicate that qRT-PCR is a reliable method to monitor shifts in cellulolytic protozoan populations. Specifically, qRT-PCR can serve as a useful monitoring technique during high-throughput screening of novel termite control agents such as cellulase inhibitors, and help to answer questions relating to whether or not such control agents impact cellulolytic protozoan populations.</description><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Cellulase</subject><subject>Cellulases - chemistry</subject><subject>Cellulases - metabolism</subject><subject>Endoglucanase</subject><subject>Enzyme Stability</subject><subject>Eukaryota - enzymology</subject><subject>Eukaryota - genetics</subject><subject>Eukaryota - radiation effects</subject><subject>Exoglucanase</subject><subject>Isoptera</subject><subject>Isoptera - genetics</subject><subject>Isoptera - parasitology</subject><subject>Isoptera - radiation effects</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoa</subject><subject>Quantitative real-time PCR</subject><subject>R. flavipes</subject><subject>Regression Analysis</subject><subject>Reticulitermes flavipes</subject><subject>Symbiosis - genetics</subject><subject>Symbiosis - radiation effects</subject><subject>Ultraviolet Rays</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rj6BzxITt66TdLpJA1eZFk_YJcF2T2HdFJxM6Y7Y9K9MP5608yANz0VFM_7UtSD0FtKWkqo-LBvwziNLSNEtmRoCSXP0I4qOTSEcfIc7cgg-oZKri7Qq1L2hBDOe_kSXVA5CNn1_Q6V2xTBrtFkbGaHx5DsI0zBmognk39CLtinjKc0hyXlMP_A7jibCuDyGPxScPLYQoxrTPG41PUhpyX9TgaHGX-HulljWCBPUIuieQoHKK_RC29igTfneYkePl_fX31tbu6-fLv6dNNY3tOloZ0wTDkJVgnh_UhkN3JnrLLDyEfHnfKdVEIqb50f6cANY9Q75Trju9657hK9P_XWm36tUBY9hbIda2ZIa9FC8b7jTPwXZIQzyugGshNocyolg9eHHOqfjpoSvTnRe7050ZsTTQZdndTQu3P7Ok7g_kbOEirw8QRAfcZTgKyLDTBbcCGDXbRL4V_9fwB_kaEW</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Wheeler, Marsha M.</creator><creator>Zhou, Xuguo</creator><creator>Scharf, Michael E.</creator><creator>Oi, Faith M.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Molecular and biochemical markers for monitoring dynamic shifts of cellulolytic protozoa in Reticulitermes flavipes</title><author>Wheeler, Marsha M. ; Zhou, Xuguo ; Scharf, Michael E. ; Oi, Faith M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-136a28d7ec866ffb073b4dac8c9b4bd4d8f378678fcdfb194a221fd8d3af35dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Cellulase</topic><topic>Cellulases - chemistry</topic><topic>Cellulases - metabolism</topic><topic>Endoglucanase</topic><topic>Enzyme Stability</topic><topic>Eukaryota - enzymology</topic><topic>Eukaryota - genetics</topic><topic>Eukaryota - radiation effects</topic><topic>Exoglucanase</topic><topic>Isoptera</topic><topic>Isoptera - genetics</topic><topic>Isoptera - parasitology</topic><topic>Isoptera - radiation effects</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoa</topic><topic>Quantitative real-time PCR</topic><topic>R. flavipes</topic><topic>Regression Analysis</topic><topic>Reticulitermes flavipes</topic><topic>Symbiosis - genetics</topic><topic>Symbiosis - radiation effects</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wheeler, Marsha M.</creatorcontrib><creatorcontrib>Zhou, Xuguo</creatorcontrib><creatorcontrib>Scharf, Michael E.</creatorcontrib><creatorcontrib>Oi, Faith M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wheeler, Marsha M.</au><au>Zhou, Xuguo</au><au>Scharf, Michael E.</au><au>Oi, Faith M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and biochemical markers for monitoring dynamic shifts of cellulolytic protozoa in Reticulitermes flavipes</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>37</volume><issue>12</issue><spage>1366</spage><epage>1374</epage><pages>1366-1374</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Lower termites rely on cellulolytic protozoa to aid in the digestion of their wood-based diet. However, despite the major contribution of protozoa to the lower termite digestive system, few techniques have been developed to monitor shifts in protozoan populations. This study investigated whether quantitative real-time PCR (qRT-PCR) and/or cellulase enzyme assays can be used to monitor changes of cellulolytic protozoan populations in the lower termite,
Reticulitermes flavipes (Kollar). Previously developed cellulase primer sets were used to test for changes in cellulase gene expression, while three different cellulase enzyme assays were used to assess changes in cellulase enzyme activity. The results from this study indicate that qRT-PCR is a reliable method to monitor shifts in cellulolytic protozoan populations. Specifically, qRT-PCR can serve as a useful monitoring technique during high-throughput screening of novel termite control agents such as cellulase inhibitors, and help to answer questions relating to whether or not such control agents impact cellulolytic protozoan populations.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17967355</pmid><doi>10.1016/j.ibmb.2007.09.010</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biomarkers - metabolism Cellulase Cellulases - chemistry Cellulases - metabolism Endoglucanase Enzyme Stability Eukaryota - enzymology Eukaryota - genetics Eukaryota - radiation effects Exoglucanase Isoptera Isoptera - genetics Isoptera - parasitology Isoptera - radiation effects Polymerase Chain Reaction Protozoa Quantitative real-time PCR R. flavipes Regression Analysis Reticulitermes flavipes Symbiosis - genetics Symbiosis - radiation effects Ultraviolet Rays |
| title | Molecular and biochemical markers for monitoring dynamic shifts of cellulolytic protozoa in Reticulitermes flavipes |
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