Detection of C-Reactive Protein Based on Immunoassay Using Antibody-Conjugated Magnetic Nanoparticles
We report a detection method for C-reactive protein (CRP) based on competitive immunoassay using magnetic nanoparticles under magnetic fields. Functional magnetic nanoparticles were prepared and conjugated with anti-CRP for immunoassay. Magnetic nanoparticles labeled with anti-CRP were flowed throug...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2007-11, Vol.79 (21), p.8416-8419 |
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| creator | Tsai, H. Y Hsu, C. F Chiu, I. W Fuh, C. Bor |
| description | We report a detection method for C-reactive protein (CRP) based on competitive immunoassay using magnetic nanoparticles under magnetic fields. Functional magnetic nanoparticles were prepared and conjugated with anti-CRP for immunoassay. Magnetic nanoparticles labeled with anti-CRP were flowed through a separation channel to form depositions for selective capture of CRP under magnetic fields. Free CRP and a fixed number of CRP-labeled particles were used to compete for a limited number of anti-CRP binding sites on the magnetic nanoparticles. The deposited percentages of CRP-labeled particles at various concentrations of free CRP were determined and used as a reference plot. The determination of CRP in the unknown sample was deduced from the reference plot using the deposited percentages. The running time was less than 10 min. The CRP concentration of serum sample was linearly over the range of 1.2−310 μg/mL for deposited percentages of CRP-labeled particles. The detection limit of this method was 0.12 μg/mL which was ∼8-fold lower than the typical clinical cutoff concentration (1 μg/mL). This method can provide a fast, simple, and sensitive way for protein detection based on competitive immunoassay using magnetic nanoparticles under magnetic fields. |
| doi_str_mv | 10.1021/ac071262n |
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The CRP concentration of serum sample was linearly over the range of 1.2−310 μg/mL for deposited percentages of CRP-labeled particles. The detection limit of this method was 0.12 μg/mL which was ∼8-fold lower than the typical clinical cutoff concentration (1 μg/mL). 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Y</creatorcontrib><creatorcontrib>Hsu, C. F</creatorcontrib><creatorcontrib>Chiu, I. W</creatorcontrib><creatorcontrib>Fuh, C. Bor</creatorcontrib><title>Detection of C-Reactive Protein Based on Immunoassay Using Antibody-Conjugated Magnetic Nanoparticles</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>We report a detection method for C-reactive protein (CRP) based on competitive immunoassay using magnetic nanoparticles under magnetic fields. Functional magnetic nanoparticles were prepared and conjugated with anti-CRP for immunoassay. Magnetic nanoparticles labeled with anti-CRP were flowed through a separation channel to form depositions for selective capture of CRP under magnetic fields. Free CRP and a fixed number of CRP-labeled particles were used to compete for a limited number of anti-CRP binding sites on the magnetic nanoparticles. The deposited percentages of CRP-labeled particles at various concentrations of free CRP were determined and used as a reference plot. The determination of CRP in the unknown sample was deduced from the reference plot using the deposited percentages. The running time was less than 10 min. The CRP concentration of serum sample was linearly over the range of 1.2−310 μg/mL for deposited percentages of CRP-labeled particles. The detection limit of this method was 0.12 μg/mL which was ∼8-fold lower than the typical clinical cutoff concentration (1 μg/mL). This method can provide a fast, simple, and sensitive way for protein detection based on competitive immunoassay using magnetic nanoparticles under magnetic fields.</description><subject>Analytical chemistry</subject><subject>Antibodies - analysis</subject><subject>Antibodies - immunology</subject><subject>Antigen-Antibody Complex</subject><subject>Binding sites</subject><subject>C-Reactive Protein - analysis</subject><subject>C-Reactive Protein - immunology</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Magnetic fields</subject><subject>Magnetics</subject><subject>Miscellaneous</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Particle Size</subject><subject>Proteins</subject><subject>Reference Values</subject><subject>Sensitivity and Specificity</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0E1v1DAQBmALgehSOPAHUIQEUg8p_khs59hd6IfUwgraCxdr4kxWWRJ7ayeI_fd4tauuBCePNY9ezQwhbxk9Z5SzT2CpYlxy94zMWMlpLrXmz8mMUipyrig9Ia9iXFPKGGXyJTlhqqJcVnpG8DOOaMfOu8y32SL_jpB-vzFbBj9i57I5RGyy1L4Zhsl5iBG22UPs3Cq7cGNX-2abL7xbTysYE7yDlcOxs9lXcH4DIZU9xtfkRQt9xDeH95Q8XH65X1znt9-ubhYXtzkUqhjz3RoVRxCgobZMKC6kYKouZF0XdbubWtuGF4KXjQRkVcErW1utOPKSQi1Oycd97ib4xwnjaIYuWux7cOinaKQuSkF1leD7f-DaT8Gl2QxnSqdEwRM62yMbfIwBW7MJ3QBhaxg1u8Obp8Mn--4QONUDNkd5uHQCHw4AooW-DeBsF4-u4rwstEwu37sujvjnqQ_hl5FKqNLcL3-Yn5fsqpyXc7M85oKNxyX-H_AvaHmk3A</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Tsai, H. 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Bor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a474t-c07192ea3a8abc137236317b46bb4bf17908cd24325d6ae19429cbc872e250ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analytical chemistry</topic><topic>Antibodies - analysis</topic><topic>Antibodies - immunology</topic><topic>Antigen-Antibody Complex</topic><topic>Binding sites</topic><topic>C-Reactive Protein - analysis</topic><topic>C-Reactive Protein - immunology</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Magnetic fields</topic><topic>Magnetics</topic><topic>Miscellaneous</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Particle Size</topic><topic>Proteins</topic><topic>Reference Values</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, H. 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Y</au><au>Hsu, C. F</au><au>Chiu, I. W</au><au>Fuh, C. Bor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of C-Reactive Protein Based on Immunoassay Using Antibody-Conjugated Magnetic Nanoparticles</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>79</volume><issue>21</issue><spage>8416</spage><epage>8419</epage><pages>8416-8419</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>We report a detection method for C-reactive protein (CRP) based on competitive immunoassay using magnetic nanoparticles under magnetic fields. Functional magnetic nanoparticles were prepared and conjugated with anti-CRP for immunoassay. Magnetic nanoparticles labeled with anti-CRP were flowed through a separation channel to form depositions for selective capture of CRP under magnetic fields. Free CRP and a fixed number of CRP-labeled particles were used to compete for a limited number of anti-CRP binding sites on the magnetic nanoparticles. The deposited percentages of CRP-labeled particles at various concentrations of free CRP were determined and used as a reference plot. The determination of CRP in the unknown sample was deduced from the reference plot using the deposited percentages. The running time was less than 10 min. The CRP concentration of serum sample was linearly over the range of 1.2−310 μg/mL for deposited percentages of CRP-labeled particles. The detection limit of this method was 0.12 μg/mL which was ∼8-fold lower than the typical clinical cutoff concentration (1 μg/mL). This method can provide a fast, simple, and sensitive way for protein detection based on competitive immunoassay using magnetic nanoparticles under magnetic fields.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17902698</pmid><doi>10.1021/ac071262n</doi><tpages>4</tpages></addata></record> |
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| subjects | Analytical chemistry Antibodies - analysis Antibodies - immunology Antigen-Antibody Complex Binding sites C-Reactive Protein - analysis C-Reactive Protein - immunology Chemistry Exact sciences and technology Humans Immunoassay Magnetic fields Magnetics Miscellaneous Nanoparticles Nanoparticles - chemistry Particle Size Proteins Reference Values Sensitivity and Specificity |
| title | Detection of C-Reactive Protein Based on Immunoassay Using Antibody-Conjugated Magnetic Nanoparticles |
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