Flexible Automated Approach for Quantitative Liquid Handling of Complex Biological Samples
A fully automated protein precipitation technique for biological sample preparation has been developed for the quantitation of drugs in various biological matrixes. All liquid handling during sample preparation was automated using a Hamilton MicroLab Star Robotic workstation, which included the prep...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2007-11, Vol.79 (21), p.8010-8015 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8015 |
|---|---|
| container_issue | 21 |
| container_start_page | 8010 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 79 |
| creator | Palandra, Joe Weller, David Hudson, Gary Li, Jeff Osgood, Sarah Hudson, Emily Zhong, Min Buchholz, Lisa Cohen, Lucinda H |
| description | A fully automated protein precipitation technique for biological sample preparation has been developed for the quantitation of drugs in various biological matrixes. All liquid handling during sample preparation was automated using a Hamilton MicroLab Star Robotic workstation, which included the preparation of standards and controls from a Watson laboratory information management system generated work list, shaking of 96-well plates, and vacuum application. Processing time is less than 30 s per sample or ∼45 min per 96-well plate, which is then immediately ready for injection onto an LC−MS/MS system. An overview of the process workflow is discussed, including the software development. Validation data are also provided, including specific liquid class data as well as comparative data of automated vs manual preparation using both quality controls and actual sample data. The efficiencies gained from this automated approach are described. |
| doi_str_mv | 10.1021/ac070618s |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68453033</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1381946561</sourcerecordid><originalsourceid>FETCH-LOGICAL-a408t-d8a526fdadd3c5ca63935e39ba86601e7a30b9c14ee8b304cd902c1b2e5a54ed3</originalsourceid><addsrcrecordid>eNpl0EFv0zAYBmALgVgZHPgDyEICiUPgsx07ybErlIGKAHVcuFhfbGd4JHFnJ2j8ezxarRKcLNmPXr1-CXnK4DUDzt6ggQoUq9M9smCSQ6Hqmt8nCwAQBa8ATsijlK4AGAOmHpITVtUN5xVfkO_r3t34tnd0OU9hwMlZutztYkDzg3Yh0q8zjpOfcPK_HN3469lbeo6j7f14SUNHV2HY5Qh65kMfLr3Bnm7x9io9Jg867JN7cjhPybf1u4vVebH5_P7DarkpsIR6KmyNkqvOorXCSINKNEI60bRYKwXMVSigbQwrnatbAaWxDXDDWu4kytJZcUpe7nNz6-vZpUkPPhnX9zi6MCet6lIKECLD5__AqzDHMXfTPC9SNSXjGb3aIxNDStF1ehf9gPG3ZqBv19Z3a2f77BA4t4OzR3mYN4MXB4ApL9NFHI1PR5eVLP82K_bOp8nd3L1j_KlVJSqpL75s9fpsu_m0Em_1x2MumnT8xP8F_wC62aH6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217879412</pqid></control><display><type>article</type><title>Flexible Automated Approach for Quantitative Liquid Handling of Complex Biological Samples</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Palandra, Joe ; Weller, David ; Hudson, Gary ; Li, Jeff ; Osgood, Sarah ; Hudson, Emily ; Zhong, Min ; Buchholz, Lisa ; Cohen, Lucinda H</creator><creatorcontrib>Palandra, Joe ; Weller, David ; Hudson, Gary ; Li, Jeff ; Osgood, Sarah ; Hudson, Emily ; Zhong, Min ; Buchholz, Lisa ; Cohen, Lucinda H</creatorcontrib><description>A fully automated protein precipitation technique for biological sample preparation has been developed for the quantitation of drugs in various biological matrixes. All liquid handling during sample preparation was automated using a Hamilton MicroLab Star Robotic workstation, which included the preparation of standards and controls from a Watson laboratory information management system generated work list, shaking of 96-well plates, and vacuum application. Processing time is less than 30 s per sample or ∼45 min per 96-well plate, which is then immediately ready for injection onto an LC−MS/MS system. An overview of the process workflow is discussed, including the software development. Validation data are also provided, including specific liquid class data as well as comparative data of automated vs manual preparation using both quality controls and actual sample data. The efficiencies gained from this automated approach are described.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac070618s</identifier><identifier>PMID: 17892272</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>2-Propanol - analysis ; Acetonitriles - analysis ; Analytical biochemistry: general aspects, technics, instrumentation ; Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Animals ; Automation ; Biological and medical sciences ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, Liquid - methods ; Chromatography, Liquid - standards ; Dimethyl Sulfoxide - analysis ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Laboratories ; Matrix ; Other chromatographic methods ; Plasma - chemistry ; Proteins ; Quality Control ; Rats ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Software ; Specimen Handling ; Spectrometric and optical methods ; Tandem Mass Spectrometry - methods ; Tandem Mass Spectrometry - standards ; Validation studies ; Water - analysis</subject><ispartof>Analytical chemistry (Washington), 2007-11, Vol.79 (21), p.8010-8015</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2008 INIST-CNRS</rights><rights>Copyright American Chemical Society Nov 1, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a408t-d8a526fdadd3c5ca63935e39ba86601e7a30b9c14ee8b304cd902c1b2e5a54ed3</citedby><cites>FETCH-LOGICAL-a408t-d8a526fdadd3c5ca63935e39ba86601e7a30b9c14ee8b304cd902c1b2e5a54ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac070618s$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac070618s$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19225433$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17892272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palandra, Joe</creatorcontrib><creatorcontrib>Weller, David</creatorcontrib><creatorcontrib>Hudson, Gary</creatorcontrib><creatorcontrib>Li, Jeff</creatorcontrib><creatorcontrib>Osgood, Sarah</creatorcontrib><creatorcontrib>Hudson, Emily</creatorcontrib><creatorcontrib>Zhong, Min</creatorcontrib><creatorcontrib>Buchholz, Lisa</creatorcontrib><creatorcontrib>Cohen, Lucinda H</creatorcontrib><title>Flexible Automated Approach for Quantitative Liquid Handling of Complex Biological Samples</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A fully automated protein precipitation technique for biological sample preparation has been developed for the quantitation of drugs in various biological matrixes. All liquid handling during sample preparation was automated using a Hamilton MicroLab Star Robotic workstation, which included the preparation of standards and controls from a Watson laboratory information management system generated work list, shaking of 96-well plates, and vacuum application. Processing time is less than 30 s per sample or ∼45 min per 96-well plate, which is then immediately ready for injection onto an LC−MS/MS system. An overview of the process workflow is discussed, including the software development. Validation data are also provided, including specific liquid class data as well as comparative data of automated vs manual preparation using both quality controls and actual sample data. The efficiencies gained from this automated approach are described.</description><subject>2-Propanol - analysis</subject><subject>Acetonitriles - analysis</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Automation</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, Liquid - methods</subject><subject>Chromatography, Liquid - standards</subject><subject>Dimethyl Sulfoxide - analysis</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Laboratories</subject><subject>Matrix</subject><subject>Other chromatographic methods</subject><subject>Plasma - chemistry</subject><subject>Proteins</subject><subject>Quality Control</subject><subject>Rats</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Software</subject><subject>Specimen Handling</subject><subject>Spectrometric and optical methods</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tandem Mass Spectrometry - standards</subject><subject>Validation studies</subject><subject>Water - analysis</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0EFv0zAYBmALgVgZHPgDyEICiUPgsx07ybErlIGKAHVcuFhfbGd4JHFnJ2j8ezxarRKcLNmPXr1-CXnK4DUDzt6ggQoUq9M9smCSQ6Hqmt8nCwAQBa8ATsijlK4AGAOmHpITVtUN5xVfkO_r3t34tnd0OU9hwMlZutztYkDzg3Yh0q8zjpOfcPK_HN3469lbeo6j7f14SUNHV2HY5Qh65kMfLr3Bnm7x9io9Jg867JN7cjhPybf1u4vVebH5_P7DarkpsIR6KmyNkqvOorXCSINKNEI60bRYKwXMVSigbQwrnatbAaWxDXDDWu4kytJZcUpe7nNz6-vZpUkPPhnX9zi6MCet6lIKECLD5__AqzDHMXfTPC9SNSXjGb3aIxNDStF1ehf9gPG3ZqBv19Z3a2f77BA4t4OzR3mYN4MXB4ApL9NFHI1PR5eVLP82K_bOp8nd3L1j_KlVJSqpL75s9fpsu_m0Em_1x2MumnT8xP8F_wC62aH6</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Palandra, Joe</creator><creator>Weller, David</creator><creator>Hudson, Gary</creator><creator>Li, Jeff</creator><creator>Osgood, Sarah</creator><creator>Hudson, Emily</creator><creator>Zhong, Min</creator><creator>Buchholz, Lisa</creator><creator>Cohen, Lucinda H</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Flexible Automated Approach for Quantitative Liquid Handling of Complex Biological Samples</title><author>Palandra, Joe ; Weller, David ; Hudson, Gary ; Li, Jeff ; Osgood, Sarah ; Hudson, Emily ; Zhong, Min ; Buchholz, Lisa ; Cohen, Lucinda H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a408t-d8a526fdadd3c5ca63935e39ba86601e7a30b9c14ee8b304cd902c1b2e5a54ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>2-Propanol - analysis</topic><topic>Acetonitriles - analysis</topic><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Automation</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, Liquid - methods</topic><topic>Chromatography, Liquid - standards</topic><topic>Dimethyl Sulfoxide - analysis</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Laboratories</topic><topic>Matrix</topic><topic>Other chromatographic methods</topic><topic>Plasma - chemistry</topic><topic>Proteins</topic><topic>Quality Control</topic><topic>Rats</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Software</topic><topic>Specimen Handling</topic><topic>Spectrometric and optical methods</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tandem Mass Spectrometry - standards</topic><topic>Validation studies</topic><topic>Water - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palandra, Joe</creatorcontrib><creatorcontrib>Weller, David</creatorcontrib><creatorcontrib>Hudson, Gary</creatorcontrib><creatorcontrib>Li, Jeff</creatorcontrib><creatorcontrib>Osgood, Sarah</creatorcontrib><creatorcontrib>Hudson, Emily</creatorcontrib><creatorcontrib>Zhong, Min</creatorcontrib><creatorcontrib>Buchholz, Lisa</creatorcontrib><creatorcontrib>Cohen, Lucinda H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palandra, Joe</au><au>Weller, David</au><au>Hudson, Gary</au><au>Li, Jeff</au><au>Osgood, Sarah</au><au>Hudson, Emily</au><au>Zhong, Min</au><au>Buchholz, Lisa</au><au>Cohen, Lucinda H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flexible Automated Approach for Quantitative Liquid Handling of Complex Biological Samples</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>79</volume><issue>21</issue><spage>8010</spage><epage>8015</epage><pages>8010-8015</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A fully automated protein precipitation technique for biological sample preparation has been developed for the quantitation of drugs in various biological matrixes. All liquid handling during sample preparation was automated using a Hamilton MicroLab Star Robotic workstation, which included the preparation of standards and controls from a Watson laboratory information management system generated work list, shaking of 96-well plates, and vacuum application. Processing time is less than 30 s per sample or ∼45 min per 96-well plate, which is then immediately ready for injection onto an LC−MS/MS system. An overview of the process workflow is discussed, including the software development. Validation data are also provided, including specific liquid class data as well as comparative data of automated vs manual preparation using both quality controls and actual sample data. The efficiencies gained from this automated approach are described.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17892272</pmid><doi>10.1021/ac070618s</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2007-11, Vol.79 (21), p.8010-8015 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68453033 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | 2-Propanol - analysis Acetonitriles - analysis Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Animals Automation Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, Liquid - methods Chromatography, Liquid - standards Dimethyl Sulfoxide - analysis Exact sciences and technology Fundamental and applied biological sciences. Psychology Laboratories Matrix Other chromatographic methods Plasma - chemistry Proteins Quality Control Rats Reference Standards Reproducibility of Results Sensitivity and Specificity Software Specimen Handling Spectrometric and optical methods Tandem Mass Spectrometry - methods Tandem Mass Spectrometry - standards Validation studies Water - analysis |
| title | Flexible Automated Approach for Quantitative Liquid Handling of Complex Biological Samples |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T10%3A43%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Flexible%20Automated%20Approach%20for%20Quantitative%20Liquid%20Handling%20of%20Complex%20Biological%20Samples&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Palandra,%20Joe&rft.date=2007-11-01&rft.volume=79&rft.issue=21&rft.spage=8010&rft.epage=8015&rft.pages=8010-8015&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac070618s&rft_dat=%3Cproquest_cross%3E1381946561%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217879412&rft_id=info:pmid/17892272&rfr_iscdi=true |