Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny
The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequenc...
Gespeichert in:
| Veröffentlicht in: | Insect biochemistry and molecular biology 2007-12, Vol.37 (12), p.1359-1365 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1365 |
|---|---|
| container_issue | 12 |
| container_start_page | 1359 |
| container_title | Insect biochemistry and molecular biology |
| container_volume | 37 |
| creator | Crone, E.J. Zera, A.J. Anand, A. Oakeshott, J.G. Sutherland, T.D. Russell, R.J. Harshman, L.G. Hoffmann, F.G. Claudianos, C. |
| description | The 458 amino acid sequence of a mature JHE protein from the cricket
Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for
G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from
G. assimilis. Partial genomic sequence encoding the
Jhe contains two large (1330 and 2918
bp) introns. No coding DNA sequence variation was observed over a 1293
bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19
bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F
2 progeny of crosses between them. Phylogenetic analyses localised the
G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade. |
| doi_str_mv | 10.1016/j.ibmb.2007.08.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68452114</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S096517480700207X</els_id><sourcerecordid>19891121</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-a1434f6e7c0e07eb81f870e091218cf8ea69013b1dbb7a36135a68bd32a225863</originalsourceid><addsrcrecordid>eNqFkE1P3DAQhq2qqGyhf6CHkhOnJowdx3aqXqpVS0FIHChny3Emi1dJvNhZpPx7HO1K3Ohp5vC88_EQ8pVCQYGKq23hmqEpGIAsQBUA1QeyokrWOTAOH8kKalHlVHJ1Sj7HuAUAziv5iZxSWQtZVnxF7m-fMHNjdh3mvt_HzMToBte7-CNb93504-Z7FvF5j6PF3NjJvbhpXihvnZmcH1NkbLPd09z7DY7zOTnpTB_xy7Gekcc_v_-t_-Z399c36193ueWymnJDeck7gdICgsRG0U7J1NaUUWU7hUbUQMuGtk0jTSloWRmhmrZkhrFKifKMXB7m7oJP18VJDy5a7Hszot9HLRSvGE1b_gfSWtU0bU0gO4A2-BgDdnoX3GDCrCnoxbfe6sW3XnxrUDr5TqFvx-n7ZsD2LXIUnICLA9AZr80muKgfH1h6DUAxCapOxM8DgUnXi8Ogo3WL7tYFtJNuvXvvglfrs5oa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19891121</pqid></control><display><type>article</type><title>Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Crone, E.J. ; Zera, A.J. ; Anand, A. ; Oakeshott, J.G. ; Sutherland, T.D. ; Russell, R.J. ; Harshman, L.G. ; Hoffmann, F.G. ; Claudianos, C.</creator><creatorcontrib>Crone, E.J. ; Zera, A.J. ; Anand, A. ; Oakeshott, J.G. ; Sutherland, T.D. ; Russell, R.J. ; Harshman, L.G. ; Hoffmann, F.G. ; Claudianos, C.</creatorcontrib><description>The 458 amino acid sequence of a mature JHE protein from the cricket
Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for
G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from
G. assimilis. Partial genomic sequence encoding the
Jhe contains two large (1330 and 2918
bp) introns. No coding DNA sequence variation was observed over a 1293
bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19
bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F
2 progeny of crosses between them. Phylogenetic analyses localised the
G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2007.08.005</identifier><identifier>PMID: 17967354</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Base Sequence ; Carboxylic Ester Hydrolases - genetics ; Carboxylic Ester Hydrolases - isolation & purification ; Carboxylic Ester Hydrolases - metabolism ; Cloning, Molecular ; complementary DNA ; enzyme activity ; Fat Body - enzymology ; Gastrointestinal Tract - enzymology ; Gene Library ; genetic polymorphism ; Gryllidae ; Gryllidae - chemistry ; Gryllidae - enzymology ; Gryllidae - genetics ; Gryllus ; Gryllus assimilis ; Hemimetabolous insect ; Hemolymph - enzymology ; Indel polymorphism ; Introns ; Isoenzymes - genetics ; Isoenzymes - isolation & purification ; Isoenzymes - metabolism ; juvenile hormone esterase ; Juvenile hormone esterase gene ; Lepidoptera ; line differences ; molecular cloning ; Molecular Probe Techniques ; Molecular Sequence Data ; nucleotide sequences ; phenology ; Phylogeny ; Selection, Genetic ; Sequence Analysis, DNA ; sequence homology ; Structure-Activity Relationship ; structure-activity relationships</subject><ispartof>Insect biochemistry and molecular biology, 2007-12, Vol.37 (12), p.1359-1365</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-a1434f6e7c0e07eb81f870e091218cf8ea69013b1dbb7a36135a68bd32a225863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S096517480700207X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17967354$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Crone, E.J.</creatorcontrib><creatorcontrib>Zera, A.J.</creatorcontrib><creatorcontrib>Anand, A.</creatorcontrib><creatorcontrib>Oakeshott, J.G.</creatorcontrib><creatorcontrib>Sutherland, T.D.</creatorcontrib><creatorcontrib>Russell, R.J.</creatorcontrib><creatorcontrib>Harshman, L.G.</creatorcontrib><creatorcontrib>Hoffmann, F.G.</creatorcontrib><creatorcontrib>Claudianos, C.</creatorcontrib><title>Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>The 458 amino acid sequence of a mature JHE protein from the cricket
Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for
G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from
G. assimilis. Partial genomic sequence encoding the
Jhe contains two large (1330 and 2918
bp) introns. No coding DNA sequence variation was observed over a 1293
bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19
bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F
2 progeny of crosses between them. Phylogenetic analyses localised the
G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>Carboxylic Ester Hydrolases - isolation & purification</subject><subject>Carboxylic Ester Hydrolases - metabolism</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>enzyme activity</subject><subject>Fat Body - enzymology</subject><subject>Gastrointestinal Tract - enzymology</subject><subject>Gene Library</subject><subject>genetic polymorphism</subject><subject>Gryllidae</subject><subject>Gryllidae - chemistry</subject><subject>Gryllidae - enzymology</subject><subject>Gryllidae - genetics</subject><subject>Gryllus</subject><subject>Gryllus assimilis</subject><subject>Hemimetabolous insect</subject><subject>Hemolymph - enzymology</subject><subject>Indel polymorphism</subject><subject>Introns</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - isolation & purification</subject><subject>Isoenzymes - metabolism</subject><subject>juvenile hormone esterase</subject><subject>Juvenile hormone esterase gene</subject><subject>Lepidoptera</subject><subject>line differences</subject><subject>molecular cloning</subject><subject>Molecular Probe Techniques</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>phenology</subject><subject>Phylogeny</subject><subject>Selection, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>sequence homology</subject><subject>Structure-Activity Relationship</subject><subject>structure-activity relationships</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq2qqGyhf6CHkhOnJowdx3aqXqpVS0FIHChny3Emi1dJvNhZpPx7HO1K3Ohp5vC88_EQ8pVCQYGKq23hmqEpGIAsQBUA1QeyokrWOTAOH8kKalHlVHJ1Sj7HuAUAziv5iZxSWQtZVnxF7m-fMHNjdh3mvt_HzMToBte7-CNb93504-Z7FvF5j6PF3NjJvbhpXihvnZmcH1NkbLPd09z7DY7zOTnpTB_xy7Gekcc_v_-t_-Z399c36193ueWymnJDeck7gdICgsRG0U7J1NaUUWU7hUbUQMuGtk0jTSloWRmhmrZkhrFKifKMXB7m7oJP18VJDy5a7Hszot9HLRSvGE1b_gfSWtU0bU0gO4A2-BgDdnoX3GDCrCnoxbfe6sW3XnxrUDr5TqFvx-n7ZsD2LXIUnICLA9AZr80muKgfH1h6DUAxCapOxM8DgUnXi8Ogo3WL7tYFtJNuvXvvglfrs5oa</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Crone, E.J.</creator><creator>Zera, A.J.</creator><creator>Anand, A.</creator><creator>Oakeshott, J.G.</creator><creator>Sutherland, T.D.</creator><creator>Russell, R.J.</creator><creator>Harshman, L.G.</creator><creator>Hoffmann, F.G.</creator><creator>Claudianos, C.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny</title><author>Crone, E.J. ; Zera, A.J. ; Anand, A. ; Oakeshott, J.G. ; Sutherland, T.D. ; Russell, R.J. ; Harshman, L.G. ; Hoffmann, F.G. ; Claudianos, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-a1434f6e7c0e07eb81f870e091218cf8ea69013b1dbb7a36135a68bd32a225863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>Carboxylic Ester Hydrolases - isolation & purification</topic><topic>Carboxylic Ester Hydrolases - metabolism</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>enzyme activity</topic><topic>Fat Body - enzymology</topic><topic>Gastrointestinal Tract - enzymology</topic><topic>Gene Library</topic><topic>genetic polymorphism</topic><topic>Gryllidae</topic><topic>Gryllidae - chemistry</topic><topic>Gryllidae - enzymology</topic><topic>Gryllidae - genetics</topic><topic>Gryllus</topic><topic>Gryllus assimilis</topic><topic>Hemimetabolous insect</topic><topic>Hemolymph - enzymology</topic><topic>Indel polymorphism</topic><topic>Introns</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - isolation & purification</topic><topic>Isoenzymes - metabolism</topic><topic>juvenile hormone esterase</topic><topic>Juvenile hormone esterase gene</topic><topic>Lepidoptera</topic><topic>line differences</topic><topic>molecular cloning</topic><topic>Molecular Probe Techniques</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>phenology</topic><topic>Phylogeny</topic><topic>Selection, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>sequence homology</topic><topic>Structure-Activity Relationship</topic><topic>structure-activity relationships</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crone, E.J.</creatorcontrib><creatorcontrib>Zera, A.J.</creatorcontrib><creatorcontrib>Anand, A.</creatorcontrib><creatorcontrib>Oakeshott, J.G.</creatorcontrib><creatorcontrib>Sutherland, T.D.</creatorcontrib><creatorcontrib>Russell, R.J.</creatorcontrib><creatorcontrib>Harshman, L.G.</creatorcontrib><creatorcontrib>Hoffmann, F.G.</creatorcontrib><creatorcontrib>Claudianos, C.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crone, E.J.</au><au>Zera, A.J.</au><au>Anand, A.</au><au>Oakeshott, J.G.</au><au>Sutherland, T.D.</au><au>Russell, R.J.</au><au>Harshman, L.G.</au><au>Hoffmann, F.G.</au><au>Claudianos, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>37</volume><issue>12</issue><spage>1359</spage><epage>1365</epage><pages>1359-1365</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>The 458 amino acid sequence of a mature JHE protein from the cricket
Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for
G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from
G. assimilis. Partial genomic sequence encoding the
Jhe contains two large (1330 and 2918
bp) introns. No coding DNA sequence variation was observed over a 1293
bp region between selected lines differing six to eight-fold in hemolymph JHE activity. However, a 19
bp indel was found in one of the introns; the insertion was strongly associated with elevated hemolymph activity, both in the selected lines and in the F
2 progeny of crosses between them. Phylogenetic analyses localised the
G. assimilis JHE to a clade containing dipteran and coleopteran JHEs, with lepidopteran JHEs occurring in a separate clade.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17967354</pmid><doi>10.1016/j.ibmb.2007.08.005</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0965-1748 |
| ispartof | Insect biochemistry and molecular biology, 2007-12, Vol.37 (12), p.1359-1365 |
| issn | 0965-1748 1879-0240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68452114 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence amino acid sequences Animals Base Sequence Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - isolation & purification Carboxylic Ester Hydrolases - metabolism Cloning, Molecular complementary DNA enzyme activity Fat Body - enzymology Gastrointestinal Tract - enzymology Gene Library genetic polymorphism Gryllidae Gryllidae - chemistry Gryllidae - enzymology Gryllidae - genetics Gryllus Gryllus assimilis Hemimetabolous insect Hemolymph - enzymology Indel polymorphism Introns Isoenzymes - genetics Isoenzymes - isolation & purification Isoenzymes - metabolism juvenile hormone esterase Juvenile hormone esterase gene Lepidoptera line differences molecular cloning Molecular Probe Techniques Molecular Sequence Data nucleotide sequences phenology Phylogeny Selection, Genetic Sequence Analysis, DNA sequence homology Structure-Activity Relationship structure-activity relationships |
| title | Jhe in Gryllus assimilis: Cloning, sequence-activity associations and phylogeny |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T18%3A01%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Jhe%20in%20Gryllus%20assimilis:%20Cloning,%20sequence-activity%20associations%20and%20phylogeny&rft.jtitle=Insect%20biochemistry%20and%20molecular%20biology&rft.au=Crone,%20E.J.&rft.date=2007-12-01&rft.volume=37&rft.issue=12&rft.spage=1359&rft.epage=1365&rft.pages=1359-1365&rft.issn=0965-1748&rft.eissn=1879-0240&rft_id=info:doi/10.1016/j.ibmb.2007.08.005&rft_dat=%3Cproquest_cross%3E19891121%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19891121&rft_id=info:pmid/17967354&rft_els_id=S096517480700207X&rfr_iscdi=true |