Rapid isolation of fungal genomic DNA suitable for long distance PCR

A quick and reliable method for screening fungal transformants for specific genetic modifications is essential for many molecular applications. We have compared the applicability of a few rapid DNA extraction methods for Myrothecium and Aspergillus and tested the resulting DNA as to its suitability...

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Veröffentlicht in:	Biotechnology letters 2007-12, Vol.29 (12), p.1845-1855
Hauptverfasser:	De Maeseneire, S. L, Van Bogaert, I. N, Dauvrin, T, Soetaert, W. K, Vandamme, E. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillus nidulans - genetics Biological and medical sciences Biotechnology Deoxyribonucleic acid DNA DNA, Fungal - isolation & purification Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Genome, Fungal Genomic DNA isolation Long distance PCR Mycelium - genetics Myrothecium gramineum Polymerase Chain Reaction - methods
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