Recombinant CD200 Protein Does Not Bind Activating Proteins Closely Related to CD200 Receptor

CD200 (OX2) is a cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. In mouse there are up to five genes related to CD200R with conflicting data as to whether they...

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Veröffentlicht in:The Journal of immunology (1950) 2005-08, Vol.175 (4), p.2469-2474
Hauptverfasser: Hatherley, Deborah, Cherwinski, Holly M, Moshref, Mehrdad, Barclay, A. Neil
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container_issue 4
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container_title The Journal of immunology (1950)
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creator Hatherley, Deborah
Cherwinski, Holly M
Moshref, Mehrdad
Barclay, A. Neil
description CD200 (OX2) is a cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. In mouse there are up to five genes related to CD200R with conflicting data as to whether they bind CD200. We show that mouse CD200 binds the inhibitory receptor CD200R with a comparable affinity (Kd = 4 microM) to those found for the rat and human CD200 CD200R interactions. CD200 gave negligible binding to the activating receptors, CD200RLa, CD200RLb, and CD200RLc, by direct analysis at the protein level using recombinant monomeric and dimeric fusion proteins or to CD200RLa and CD200RLb when expressed at the cell surface. An additional potential activating gene, CD200RLe, found in only some mouse strains also did not bind CD200. Thus, the CD200 receptor family consists of both activatory and inhibitory members like several other paired ligand receptors, such as signal regulatory protein, killer cell Ig-like receptor/KAR, LY49, dendritic cell immunoreceptor/dendritic cell immunoactivating receptor, and paired Ig-like type 2 receptor. Although the ligand for the inhibitory product is a widely distributed host protein, the ligands of the activating forms remain to be identified, and one possibility is that they are pathogen components.
doi_str_mv 10.4049/jimmunol.175.4.2469
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subjects Amino Acid Sequence
Animals
Antigens, CD - biosynthesis
Antigens, CD - genetics
Antigens, CD - metabolism
Antigens, Surface - biosynthesis
Antigens, Surface - genetics
Antigens, Surface - metabolism
Antigens, Surface - physiology
Cell Line
Cell Membrane - immunology
Cell Membrane - metabolism
Humans
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Membrane Glycoproteins - physiology
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Orexin Receptors
Protein Interaction Mapping
Protein Isoforms - genetics
Protein Isoforms - metabolism
Rats
Receptors, Cell Surface - biosynthesis
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Receptors, Cell Surface - physiology
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
title Recombinant CD200 Protein Does Not Bind Activating Proteins Closely Related to CD200 Receptor
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