Hypoxia Alters Cathepsin B / Inhibitor Profiles in Oral Carcinoma Cell Lines
Background: The tumor microenvironment is believed to contribute to the malignant properties of tumor cells in heterogeneous tumor tissues. We investigated the impact of hypoxia (1% oxygen) on the expression of cathepsin B and its natural inhibitors cystatin B and C. Materials and Methods: Patient-m...
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| Veröffentlicht in: | Anticancer research 2005-07, Vol.25 (4), p.2841-2849 |
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| creator | WICKRAMASINGHE, Nalinie S BANERJEE, Kasturi NAGARAJ, Nagathihalli S VIGNESWARAN, Nadarajah ZACHARIAS, Wolfgang |
| description | Background: The tumor microenvironment is believed to contribute to the malignant properties of tumor cells in heterogeneous
tumor tissues. We investigated the impact of hypoxia (1% oxygen) on the expression of cathepsin B and its natural inhibitors
cystatin B and C. Materials and Methods: Patient-matched oral carcinoma cell lines from primary tumor and lymph node metastasis
were used to study the effects of hypoxia on proliferation, protein expression, and proteolytic and inhibitor activities.
Results: Hypoxic growth led to elevated cathepsin B expression and activity, and this effect was greater in metastatic than
in primary tumor cells. Also, hypoxia led to down-regulation of the inhibitors cystatin C and B, resulting in increased residual
activity of cathepsin B. Conclusion: These data suggest that the invasive and/or metastatic potential of cells may be enhanced
under hypoxia by increasing cathepsin-mediated proteolysis. The results provide strong evidence for the involvement of cathepsin
B and its cystatin inhibitors in hypoxia-enhanced tumor progression. |
| format | Article |
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tumor tissues. We investigated the impact of hypoxia (1% oxygen) on the expression of cathepsin B and its natural inhibitors
cystatin B and C. Materials and Methods: Patient-matched oral carcinoma cell lines from primary tumor and lymph node metastasis
were used to study the effects of hypoxia on proliferation, protein expression, and proteolytic and inhibitor activities.
Results: Hypoxic growth led to elevated cathepsin B expression and activity, and this effect was greater in metastatic than
in primary tumor cells. Also, hypoxia led to down-regulation of the inhibitors cystatin C and B, resulting in increased residual
activity of cathepsin B. Conclusion: These data suggest that the invasive and/or metastatic potential of cells may be enhanced
under hypoxia by increasing cathepsin-mediated proteolysis. The results provide strong evidence for the involvement of cathepsin
B and its cystatin inhibitors in hypoxia-enhanced tumor progression.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 16080536</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Aged ; Biological and medical sciences ; Carcinoma, Squamous Cell - enzymology ; Carcinoma, Squamous Cell - pathology ; Cathepsin B - antagonists & inhibitors ; Cathepsin B - metabolism ; Cell Growth Processes - physiology ; Cell Hypoxia - physiology ; Cell Line, Tumor ; Cystatin B ; Cystatin C ; Cystatins - metabolism ; Cysteine Proteinase Inhibitors - metabolism ; Humans ; Hypopharyngeal Neoplasms - enzymology ; Hypopharyngeal Neoplasms - pathology ; Male ; Medical sciences ; Middle Aged ; Mouth Neoplasms - enzymology ; Mouth Neoplasms - pathology ; Otorhinolaryngology. Stomatology ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Anticancer research, 2005-07, Vol.25 (4), p.2841-2849</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16976278$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16080536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WICKRAMASINGHE, Nalinie S</creatorcontrib><creatorcontrib>BANERJEE, Kasturi</creatorcontrib><creatorcontrib>NAGARAJ, Nagathihalli S</creatorcontrib><creatorcontrib>VIGNESWARAN, Nadarajah</creatorcontrib><creatorcontrib>ZACHARIAS, Wolfgang</creatorcontrib><title>Hypoxia Alters Cathepsin B / Inhibitor Profiles in Oral Carcinoma Cell Lines</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: The tumor microenvironment is believed to contribute to the malignant properties of tumor cells in heterogeneous
tumor tissues. We investigated the impact of hypoxia (1% oxygen) on the expression of cathepsin B and its natural inhibitors
cystatin B and C. Materials and Methods: Patient-matched oral carcinoma cell lines from primary tumor and lymph node metastasis
were used to study the effects of hypoxia on proliferation, protein expression, and proteolytic and inhibitor activities.
Results: Hypoxic growth led to elevated cathepsin B expression and activity, and this effect was greater in metastatic than
in primary tumor cells. Also, hypoxia led to down-regulation of the inhibitors cystatin C and B, resulting in increased residual
activity of cathepsin B. Conclusion: These data suggest that the invasive and/or metastatic potential of cells may be enhanced
under hypoxia by increasing cathepsin-mediated proteolysis. The results provide strong evidence for the involvement of cathepsin
B and its cystatin inhibitors in hypoxia-enhanced tumor progression.</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - enzymology</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cathepsin B - antagonists & inhibitors</subject><subject>Cathepsin B - metabolism</subject><subject>Cell Growth Processes - physiology</subject><subject>Cell Hypoxia - physiology</subject><subject>Cell Line, Tumor</subject><subject>Cystatin B</subject><subject>Cystatin C</subject><subject>Cystatins - metabolism</subject><subject>Cysteine Proteinase Inhibitors - metabolism</subject><subject>Humans</subject><subject>Hypopharyngeal Neoplasms - enzymology</subject><subject>Hypopharyngeal Neoplasms - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mouth Neoplasms - enzymology</subject><subject>Mouth Neoplasms - pathology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNz81KxDAUBeAgijOOvoJko7timjRpshyLOgOFcaHrkLS3NpL-mHTQeXsLjujqLs7H4dwTtExzlSY5Z-QULQnlJMkJ4Qt0EeM7IUIoyc7RIhVEEs7EEpWbwzh8OYPXfoIQcWGmFsboenyP7_C2b5110xDwcxga5yHiOdkF42cYKtcPncEFeI9L10O8RGeN8RGujneFXh8fXopNUu6etsW6TFoq1JTQ1ObKSgv1vKEmpDGMSoC6VlUqBTcNzyRXAixvILXUGqYsiCarYE4rVrMVuv3pHcPwsYc46c7Fap5hehj2UQuZZbkidIbXR7i3HdR6DK4z4aB__5_BzRGYWBnfBNNXLv5zKhc0l3-udW_tpwugY2e8n2uZNoFynWkqs5R9A-skcMw</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>WICKRAMASINGHE, Nalinie S</creator><creator>BANERJEE, Kasturi</creator><creator>NAGARAJ, Nagathihalli S</creator><creator>VIGNESWARAN, Nadarajah</creator><creator>ZACHARIAS, Wolfgang</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20050701</creationdate><title>Hypoxia Alters Cathepsin B / Inhibitor Profiles in Oral Carcinoma Cell Lines</title><author>WICKRAMASINGHE, Nalinie S ; BANERJEE, Kasturi ; NAGARAJ, Nagathihalli S ; VIGNESWARAN, Nadarajah ; ZACHARIAS, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-21b79b8bed053d00fa328eedd9c1865af548596eb5fe1b2ba39be6f4ce865c3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - enzymology</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Cathepsin B - antagonists & inhibitors</topic><topic>Cathepsin B - metabolism</topic><topic>Cell Growth Processes - physiology</topic><topic>Cell Hypoxia - physiology</topic><topic>Cell Line, Tumor</topic><topic>Cystatin B</topic><topic>Cystatin C</topic><topic>Cystatins - metabolism</topic><topic>Cysteine Proteinase Inhibitors - metabolism</topic><topic>Humans</topic><topic>Hypopharyngeal Neoplasms - enzymology</topic><topic>Hypopharyngeal Neoplasms - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mouth Neoplasms - enzymology</topic><topic>Mouth Neoplasms - pathology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WICKRAMASINGHE, Nalinie S</creatorcontrib><creatorcontrib>BANERJEE, Kasturi</creatorcontrib><creatorcontrib>NAGARAJ, Nagathihalli S</creatorcontrib><creatorcontrib>VIGNESWARAN, Nadarajah</creatorcontrib><creatorcontrib>ZACHARIAS, Wolfgang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WICKRAMASINGHE, Nalinie S</au><au>BANERJEE, Kasturi</au><au>NAGARAJ, Nagathihalli S</au><au>VIGNESWARAN, Nadarajah</au><au>ZACHARIAS, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypoxia Alters Cathepsin B / Inhibitor Profiles in Oral Carcinoma Cell Lines</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2005-07-01</date><risdate>2005</risdate><volume>25</volume><issue>4</issue><spage>2841</spage><epage>2849</epage><pages>2841-2849</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: The tumor microenvironment is believed to contribute to the malignant properties of tumor cells in heterogeneous
tumor tissues. We investigated the impact of hypoxia (1% oxygen) on the expression of cathepsin B and its natural inhibitors
cystatin B and C. Materials and Methods: Patient-matched oral carcinoma cell lines from primary tumor and lymph node metastasis
were used to study the effects of hypoxia on proliferation, protein expression, and proteolytic and inhibitor activities.
Results: Hypoxic growth led to elevated cathepsin B expression and activity, and this effect was greater in metastatic than
in primary tumor cells. Also, hypoxia led to down-regulation of the inhibitors cystatin C and B, resulting in increased residual
activity of cathepsin B. Conclusion: These data suggest that the invasive and/or metastatic potential of cells may be enhanced
under hypoxia by increasing cathepsin-mediated proteolysis. The results provide strong evidence for the involvement of cathepsin
B and its cystatin inhibitors in hypoxia-enhanced tumor progression.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>16080536</pmid><tpages>9</tpages></addata></record> |
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| subjects | Aged Biological and medical sciences Carcinoma, Squamous Cell - enzymology Carcinoma, Squamous Cell - pathology Cathepsin B - antagonists & inhibitors Cathepsin B - metabolism Cell Growth Processes - physiology Cell Hypoxia - physiology Cell Line, Tumor Cystatin B Cystatin C Cystatins - metabolism Cysteine Proteinase Inhibitors - metabolism Humans Hypopharyngeal Neoplasms - enzymology Hypopharyngeal Neoplasms - pathology Male Medical sciences Middle Aged Mouth Neoplasms - enzymology Mouth Neoplasms - pathology Otorhinolaryngology. Stomatology Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Hypoxia Alters Cathepsin B / Inhibitor Profiles in Oral Carcinoma Cell Lines |
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