Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells
The nonobese diabetic/severe combined immune deficiency (NOD/SCID) xenotransplantation model has emerged as a widely used assay for human hematopoietic stem cells; however, barriers still exist that limit engraftment. We previously identified a short-term SCID-repopulating cell (SRC) following direc...
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| Veröffentlicht in: | Blood 2005-08, Vol.106 (4), p.1259-1261 |
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| creator | McKenzie, Joby L. Gan, Olga I. Doedens, Monica Dick, John E. |
| description | The nonobese diabetic/severe combined immune deficiency (NOD/SCID) xenotransplantation model has emerged as a widely used assay for human hematopoietic stem cells; however, barriers still exist that limit engraftment. We previously identified a short-term SCID-repopulating cell (SRC) following direct intrafemoral injection into NOD/SCID mice, whereas others characterized similar SRCs using NOD/SCID mice depleted of natural killer (NK) cell activity. To determine the model that most efficiently detects short-term SRCs, we compared human engraftment in 6 different xenotransplantation models: NOD/SCID-β2-microglobulin-null mice, anti-CD122 (interleukin-2 receptor β [IL-2Rβ])–treated or unmanipulated NOD/SCID mice, each given transplants by intravenous or intrafemoral injection. Human cell engraftment was highest in intrafemorally injected anti-CD122–treated NOD/SCID mice compared to all other groups at 2 and 6 weeks after transplantation. These modifications to the SRC assay provide improved detection of human stem cells and demonstrate that CD122+ cells provide barriers to stem cell engraftment, a finding with potential clinical relevance. |
| doi_str_mv | 10.1182/blood-2005-03-1081 |
| format | Article |
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We previously identified a short-term SCID-repopulating cell (SRC) following direct intrafemoral injection into NOD/SCID mice, whereas others characterized similar SRCs using NOD/SCID mice depleted of natural killer (NK) cell activity. To determine the model that most efficiently detects short-term SRCs, we compared human engraftment in 6 different xenotransplantation models: NOD/SCID-β2-microglobulin-null mice, anti-CD122 (interleukin-2 receptor β [IL-2Rβ])–treated or unmanipulated NOD/SCID mice, each given transplants by intravenous or intrafemoral injection. Human cell engraftment was highest in intrafemorally injected anti-CD122–treated NOD/SCID mice compared to all other groups at 2 and 6 weeks after transplantation. These modifications to the SRC assay provide improved detection of human stem cells and demonstrate that CD122+ cells provide barriers to stem cell engraftment, a finding with potential clinical relevance.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood-2005-03-1081</identifier><identifier>PMID: 15878972</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antibodies, Monoclonal - administration & dosage ; Antibodies, Monoclonal - pharmacology ; beta 2-Microglobulin - deficiency ; Femur ; Hematopoiesis ; Hematopoietic Stem Cell Transplantation - methods ; Hematopoietic Stem Cell Transplantation - standards ; Hematopoietic Stem Cells - cytology ; Humans ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Models, Animal ; Receptors, Interleukin-2 - deficiency ; Receptors, Interleukin-2 - immunology ; Receptors, Interleukin-2 - physiology ; Transplantation, Heterologous</subject><ispartof>Blood, 2005-08, Vol.106 (4), p.1259-1261</ispartof><rights>2005 American Society of Hematology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-33d1bd632390828547e9f3e5a0845cbefa9f1a8a33b8a9e4699382b47a546a9b3</citedby><cites>FETCH-LOGICAL-c398t-33d1bd632390828547e9f3e5a0845cbefa9f1a8a33b8a9e4699382b47a546a9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15878972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McKenzie, Joby L.</creatorcontrib><creatorcontrib>Gan, Olga I.</creatorcontrib><creatorcontrib>Doedens, Monica</creatorcontrib><creatorcontrib>Dick, John E.</creatorcontrib><title>Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells</title><title>Blood</title><addtitle>Blood</addtitle><description>The nonobese diabetic/severe combined immune deficiency (NOD/SCID) xenotransplantation model has emerged as a widely used assay for human hematopoietic stem cells; however, barriers still exist that limit engraftment. We previously identified a short-term SCID-repopulating cell (SRC) following direct intrafemoral injection into NOD/SCID mice, whereas others characterized similar SRCs using NOD/SCID mice depleted of natural killer (NK) cell activity. To determine the model that most efficiently detects short-term SRCs, we compared human engraftment in 6 different xenotransplantation models: NOD/SCID-β2-microglobulin-null mice, anti-CD122 (interleukin-2 receptor β [IL-2Rβ])–treated or unmanipulated NOD/SCID mice, each given transplants by intravenous or intrafemoral injection. Human cell engraftment was highest in intrafemorally injected anti-CD122–treated NOD/SCID mice compared to all other groups at 2 and 6 weeks after transplantation. These modifications to the SRC assay provide improved detection of human stem cells and demonstrate that CD122+ cells provide barriers to stem cell engraftment, a finding with potential clinical relevance.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - administration & dosage</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>beta 2-Microglobulin - deficiency</subject><subject>Femur</subject><subject>Hematopoiesis</subject><subject>Hematopoietic Stem Cell Transplantation - methods</subject><subject>Hematopoietic Stem Cell Transplantation - standards</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Models, Animal</subject><subject>Receptors, Interleukin-2 - deficiency</subject><subject>Receptors, Interleukin-2 - immunology</subject><subject>Receptors, Interleukin-2 - physiology</subject><subject>Transplantation, Heterologous</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQhy1ERZfCC3BAPnFBpv6XxJa4oN1CK1X0AJwtJxmDkRMH2wH1VXjaOuxK3HqyJf_mm_F8CL1i9B1jil_2IcaRcEobQgVhVLEnaMcargilnD5FO0ppS6Tu2Dl6nvNPSpkUvHmGzlmjOqU7vkN_r9fJzjj_iKmQAmnCCZa4rMEWP3_HucCEBwghY5sAg3N-8DCXcI9HKDAUGLGLIcQ_W9rPJVkHU0w24Hqd8xLsXCoqzttjxJ_vDpdf9jeH2mXwy0bKFbQE2EDR4f2Bcf722PEFOnM2ZHh5Oi_Qt49XX_fX5Pbu083-wy0ZhFaFCDGyfmwFF5oqrhrZgXYCGkuVbIYenNWOWWWF6JXVIFutheK97GwjW6t7cYHeHLlLir9WyMVMPm8T2Bnimk2rpJS00zXIj8EhxZwTOLMkP9l0bxg1mxHzz4jZjBgqzGakFr0-0dd-gvF_yUlBDbw_BqD-8beHZPK24gFGX5dUzBj9Y_wH8wae7g</recordid><startdate>20050815</startdate><enddate>20050815</enddate><creator>McKenzie, Joby L.</creator><creator>Gan, Olga I.</creator><creator>Doedens, Monica</creator><creator>Dick, John E.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050815</creationdate><title>Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells</title><author>McKenzie, Joby L. ; Gan, Olga I. ; Doedens, Monica ; Dick, John E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-33d1bd632390828547e9f3e5a0845cbefa9f1a8a33b8a9e4699382b47a546a9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - administration & dosage</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>beta 2-Microglobulin - deficiency</topic><topic>Femur</topic><topic>Hematopoiesis</topic><topic>Hematopoietic Stem Cell Transplantation - methods</topic><topic>Hematopoietic Stem Cell Transplantation - standards</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Mice, SCID</topic><topic>Models, Animal</topic><topic>Receptors, Interleukin-2 - deficiency</topic><topic>Receptors, Interleukin-2 - immunology</topic><topic>Receptors, Interleukin-2 - physiology</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McKenzie, Joby L.</creatorcontrib><creatorcontrib>Gan, Olga I.</creatorcontrib><creatorcontrib>Doedens, Monica</creatorcontrib><creatorcontrib>Dick, John E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McKenzie, Joby L.</au><au>Gan, Olga I.</au><au>Doedens, Monica</au><au>Dick, John E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>2005-08-15</date><risdate>2005</risdate><volume>106</volume><issue>4</issue><spage>1259</spage><epage>1261</epage><pages>1259-1261</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>The nonobese diabetic/severe combined immune deficiency (NOD/SCID) xenotransplantation model has emerged as a widely used assay for human hematopoietic stem cells; however, barriers still exist that limit engraftment. We previously identified a short-term SCID-repopulating cell (SRC) following direct intrafemoral injection into NOD/SCID mice, whereas others characterized similar SRCs using NOD/SCID mice depleted of natural killer (NK) cell activity. To determine the model that most efficiently detects short-term SRCs, we compared human engraftment in 6 different xenotransplantation models: NOD/SCID-β2-microglobulin-null mice, anti-CD122 (interleukin-2 receptor β [IL-2Rβ])–treated or unmanipulated NOD/SCID mice, each given transplants by intravenous or intrafemoral injection. Human cell engraftment was highest in intrafemorally injected anti-CD122–treated NOD/SCID mice compared to all other groups at 2 and 6 weeks after transplantation. These modifications to the SRC assay provide improved detection of human stem cells and demonstrate that CD122+ cells provide barriers to stem cell engraftment, a finding with potential clinical relevance.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15878972</pmid><doi>10.1182/blood-2005-03-1081</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal - administration & dosage Antibodies, Monoclonal - pharmacology beta 2-Microglobulin - deficiency Femur Hematopoiesis Hematopoietic Stem Cell Transplantation - methods Hematopoietic Stem Cell Transplantation - standards Hematopoietic Stem Cells - cytology Humans Mice Mice, Inbred NOD Mice, SCID Models, Animal Receptors, Interleukin-2 - deficiency Receptors, Interleukin-2 - immunology Receptors, Interleukin-2 - physiology Transplantation, Heterologous |
| title | Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells |
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