Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p
Transcriptional activators work by recruiting transcription factors that are required for the process of transcription to their target genes. We have used the Split-Ubiquitin system to identify eight transcription factors that interacted with both the transcriptional activators Gal4p and Gcn4p in li...
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description | Transcriptional activators work by recruiting transcription factors that are required for the process of transcription to their target genes. We have used the Split-Ubiquitin system to identify eight transcription factors that interacted with both the transcriptional activators Gal4p and Gcn4p in living cells. The over-expression of one of the activator-interacting proteins, Gal11p, partially suppressed
GAL4 and
GCN4 deletions. We have isolated two point mutants in Gal11p, F848L and F869S that were defective for the dosage compensation. We have identified 35 transcription factors that interacted with Gal11p in living cells, and the only protein–protein interaction affected by the Gal11p mutations was the one between Gal11p and Taf14p. We have further shown that the suppression of a
GAL4 deletion by high levels of Gal11p required Taf14p, and that over-expression of Gal11p recruited Taf14p to the
GAL1 promoter together with Tbp1p, Swi2p and Srb7p. Gal11p interacted with Mig1p, indicating that Mig1/2p could have recruited Gal11p to the
GAL1 promoter in the absence of Gal4p. Our results suggest that transcriptional activators work by raising the local concentration of the limiting factor Gal11p, and that Gal11p works by recruiting Mediator and Taf14p-containing transcription factors like TFIID and SWI/SNF and by competing general repressors like Ssn6p-Tup1p off the target promoters. |
doi_str_mv | 10.1016/j.jmb.2007.09.013 |
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GAL4 and
GCN4 deletions. We have isolated two point mutants in Gal11p, F848L and F869S that were defective for the dosage compensation. We have identified 35 transcription factors that interacted with Gal11p in living cells, and the only protein–protein interaction affected by the Gal11p mutations was the one between Gal11p and Taf14p. We have further shown that the suppression of a
GAL4 deletion by high levels of Gal11p required Taf14p, and that over-expression of Gal11p recruited Taf14p to the
GAL1 promoter together with Tbp1p, Swi2p and Srb7p. Gal11p interacted with Mig1p, indicating that Mig1/2p could have recruited Gal11p to the
GAL1 promoter in the absence of Gal4p. Our results suggest that transcriptional activators work by raising the local concentration of the limiting factor Gal11p, and that Gal11p works by recruiting Mediator and Taf14p-containing transcription factors like TFIID and SWI/SNF and by competing general repressors like Ssn6p-Tup1p off the target promoters.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/j.jmb.2007.09.013</identifier><identifier>PMID: 17919657</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>activation ; Adenosine Triphosphatases ; Blotting, Northern ; Chromatin - metabolism ; DNA, Fungal ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Galactokinase - genetics ; Galactokinase - metabolism ; Gene Dosage ; Gene Expression Regulation, Fungal ; Mediator Complex ; Plasmids ; Promoter Regions, Genetic - genetics ; Protein Binding ; protein interaction ; recruitment ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae Proteins - chemistry ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Sequence Deletion ; Split-Ubiquitin ; Trans-Activators - genetics ; Trans-Activators - metabolism ; transcription ; Transcription Factor TFIID - genetics ; Transcription Factor TFIID - metabolism ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic</subject><ispartof>Journal of molecular biology, 2007-11, Vol.374 (1), p.9-23</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c297t-788c7dffe372cc59f755fe9b12ed7d2db41b62cb160e11cddb93f64580fdfa0d3</citedby><cites>FETCH-LOGICAL-c297t-788c7dffe372cc59f755fe9b12ed7d2db41b62cb160e11cddb93f64580fdfa0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jmb.2007.09.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17919657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Mei Kee</creatorcontrib><creatorcontrib>Tang, Vivien</creatorcontrib><creatorcontrib>Le Saux, Agnès</creatorcontrib><creatorcontrib>Schüller, Jutta</creatorcontrib><creatorcontrib>Bongards, Christine</creatorcontrib><creatorcontrib>Lehming, Norbert</creatorcontrib><title>Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Transcriptional activators work by recruiting transcription factors that are required for the process of transcription to their target genes. We have used the Split-Ubiquitin system to identify eight transcription factors that interacted with both the transcriptional activators Gal4p and Gcn4p in living cells. The over-expression of one of the activator-interacting proteins, Gal11p, partially suppressed
GAL4 and
GCN4 deletions. We have isolated two point mutants in Gal11p, F848L and F869S that were defective for the dosage compensation. We have identified 35 transcription factors that interacted with Gal11p in living cells, and the only protein–protein interaction affected by the Gal11p mutations was the one between Gal11p and Taf14p. We have further shown that the suppression of a
GAL4 deletion by high levels of Gal11p required Taf14p, and that over-expression of Gal11p recruited Taf14p to the
GAL1 promoter together with Tbp1p, Swi2p and Srb7p. Gal11p interacted with Mig1p, indicating that Mig1/2p could have recruited Gal11p to the
GAL1 promoter in the absence of Gal4p. Our results suggest that transcriptional activators work by raising the local concentration of the limiting factor Gal11p, and that Gal11p works by recruiting Mediator and Taf14p-containing transcription factors like TFIID and SWI/SNF and by competing general repressors like Ssn6p-Tup1p off the target promoters.</description><subject>activation</subject><subject>Adenosine Triphosphatases</subject><subject>Blotting, Northern</subject><subject>Chromatin - metabolism</subject><subject>DNA, Fungal</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Galactokinase - genetics</subject><subject>Galactokinase - metabolism</subject><subject>Gene Dosage</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Mediator Complex</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>protein interaction</subject><subject>recruitment</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae Proteins - chemistry</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Sequence Deletion</subject><subject>Split-Ubiquitin</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>transcription</subject><subject>Transcription Factor TFIID - genetics</subject><subject>Transcription Factor TFIID - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAURkVpSaZpfkA2wavu7N4r23qQVUjSpBDoZroWsnRVNPhVyTOQfx8PM9Bdu7pwOd9ZHMZuECoEFN921W7oKg4gK9AVYP2BbRCULpWo1Ue2AeC85KoWl-xzzjsAaOtGXbBLlBq1aOWGvTzbHnEuHqdsf1PppmGmMduFcrFNdswuxXmJ02j74t4t8WCXKRWP1NPxmYtDtMXWBmzmL-xTsH2m6_O9Yr--P20fXsrXn88_Hu5fS8e1XEqplJM-BKold67VQbZtIN0hJy89912DneCuQwGE6LzvdB1E0yoIPljw9RX7evLOafqzp7yYIWZHfW9HmvbZCNU0dYvyvyAHKRspmhXEE-jSlHOiYOYUB5veDII5djY7s3Y2x84GtFk7r5vbs3zfDeT_Ls5hV-DuBNDa4hApmewijY58TOQW46f4D_07kA2OHw</recordid><startdate>20071116</startdate><enddate>20071116</enddate><creator>Lim, Mei Kee</creator><creator>Tang, Vivien</creator><creator>Le Saux, Agnès</creator><creator>Schüller, Jutta</creator><creator>Bongards, Christine</creator><creator>Lehming, Norbert</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20071116</creationdate><title>Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p</title><author>Lim, Mei Kee ; Tang, Vivien ; Le Saux, Agnès ; Schüller, Jutta ; Bongards, Christine ; Lehming, Norbert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-788c7dffe372cc59f755fe9b12ed7d2db41b62cb160e11cddb93f64580fdfa0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>activation</topic><topic>Adenosine Triphosphatases</topic><topic>Blotting, Northern</topic><topic>Chromatin - metabolism</topic><topic>DNA, Fungal</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Galactokinase - genetics</topic><topic>Galactokinase - metabolism</topic><topic>Gene Dosage</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Mediator Complex</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>protein interaction</topic><topic>recruitment</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae Proteins - chemistry</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Sequence Deletion</topic><topic>Split-Ubiquitin</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>transcription</topic><topic>Transcription Factor TFIID - genetics</topic><topic>Transcription Factor TFIID - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Mei Kee</creatorcontrib><creatorcontrib>Tang, Vivien</creatorcontrib><creatorcontrib>Le Saux, Agnès</creatorcontrib><creatorcontrib>Schüller, Jutta</creatorcontrib><creatorcontrib>Bongards, Christine</creatorcontrib><creatorcontrib>Lehming, Norbert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Mei Kee</au><au>Tang, Vivien</au><au>Le Saux, Agnès</au><au>Schüller, Jutta</au><au>Bongards, Christine</au><au>Lehming, Norbert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2007-11-16</date><risdate>2007</risdate><volume>374</volume><issue>1</issue><spage>9</spage><epage>23</epage><pages>9-23</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>Transcriptional activators work by recruiting transcription factors that are required for the process of transcription to their target genes. We have used the Split-Ubiquitin system to identify eight transcription factors that interacted with both the transcriptional activators Gal4p and Gcn4p in living cells. The over-expression of one of the activator-interacting proteins, Gal11p, partially suppressed
GAL4 and
GCN4 deletions. We have isolated two point mutants in Gal11p, F848L and F869S that were defective for the dosage compensation. We have identified 35 transcription factors that interacted with Gal11p in living cells, and the only protein–protein interaction affected by the Gal11p mutations was the one between Gal11p and Taf14p. We have further shown that the suppression of a
GAL4 deletion by high levels of Gal11p required Taf14p, and that over-expression of Gal11p recruited Taf14p to the
GAL1 promoter together with Tbp1p, Swi2p and Srb7p. Gal11p interacted with Mig1p, indicating that Mig1/2p could have recruited Gal11p to the
GAL1 promoter in the absence of Gal4p. Our results suggest that transcriptional activators work by raising the local concentration of the limiting factor Gal11p, and that Gal11p works by recruiting Mediator and Taf14p-containing transcription factors like TFIID and SWI/SNF and by competing general repressors like Ssn6p-Tup1p off the target promoters.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17919657</pmid><doi>10.1016/j.jmb.2007.09.013</doi><tpages>15</tpages></addata></record> |
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subjects | activation Adenosine Triphosphatases Blotting, Northern Chromatin - metabolism DNA, Fungal DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Galactokinase - genetics Galactokinase - metabolism Gene Dosage Gene Expression Regulation, Fungal Mediator Complex Plasmids Promoter Regions, Genetic - genetics Protein Binding protein interaction recruitment Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Sequence Deletion Split-Ubiquitin Trans-Activators - genetics Trans-Activators - metabolism transcription Transcription Factor TFIID - genetics Transcription Factor TFIID - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic |
title | Gal11p Dosage-compensates Transcriptional Activator Deletions via Taf14p |
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