Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa
Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake...
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Veröffentlicht in: | Reproduction (Cambridge, England) England), 2007-11, Vol.134 (5), p.651-658 |
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creator | Kobayashi, Daisuke Tamai, Ikumi Sai, Yoshimichi Yoshida, Kazuhiro Wakayama, Tomohiko Kido, Yasuto Nezu, Jun-ichi Iseki, Shoichi Tsuji, Akira |
description | Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na+-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with Km values of 23.6 and 6.57 μM for carnitine and acetylcarnitine respectively in the presence of Na+. Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na+-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice. |
doi_str_mv | 10.1530/REP-06-0173 |
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In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na+-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with Km values of 23.6 and 6.57 μM for carnitine and acetylcarnitine respectively in the presence of Na+. Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na+-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.</description><identifier>ISSN: 1470-1626</identifier><identifier>EISSN: 1741-7899</identifier><identifier>DOI: 10.1530/REP-06-0173</identifier><identifier>PMID: 17965255</identifier><language>eng</language><publisher>England: Society for Reproduction and Fertility</publisher><subject>Acetylcarnitine - pharmacokinetics ; Animals ; Betaine - analogs & derivatives ; Betaine - pharmacology ; Biological Transport, Active ; Carnitine - pharmacokinetics ; Carnitine - pharmacology ; Epididymis ; Fluorescent Antibody Technique ; Male ; Membrane Proteins - analysis ; Membrane Proteins - antagonists & inhibitors ; Membrane Proteins - metabolism ; Mice ; Organic Cation Transport Proteins - analysis ; Organic Cation Transport Proteins - antagonists & inhibitors ; Organic Cation Transport Proteins - metabolism ; Pyrilamine - pharmacology ; Solute Carrier Family 22 Member 5 ; Sperm Maturation - physiology ; Sperm Motility - physiology ; Spermatozoa - metabolism</subject><ispartof>Reproduction (Cambridge, England), 2007-11, Vol.134 (5), p.651-658</ispartof><rights>2007 Society for Reproduction and Fertility</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b465t-9879cc13d37cdb525de5f1b616c0229cbd4959ef3a3a511c888992b696cad20a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17965255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobayashi, Daisuke</creatorcontrib><creatorcontrib>Tamai, Ikumi</creatorcontrib><creatorcontrib>Sai, Yoshimichi</creatorcontrib><creatorcontrib>Yoshida, Kazuhiro</creatorcontrib><creatorcontrib>Wakayama, Tomohiko</creatorcontrib><creatorcontrib>Kido, Yasuto</creatorcontrib><creatorcontrib>Nezu, Jun-ichi</creatorcontrib><creatorcontrib>Iseki, Shoichi</creatorcontrib><creatorcontrib>Tsuji, Akira</creatorcontrib><title>Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa</title><title>Reproduction (Cambridge, England)</title><addtitle>Reproduction</addtitle><description>Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na+-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with Km values of 23.6 and 6.57 μM for carnitine and acetylcarnitine respectively in the presence of Na+. Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na+-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.</description><subject>Acetylcarnitine - pharmacokinetics</subject><subject>Animals</subject><subject>Betaine - analogs & derivatives</subject><subject>Betaine - pharmacology</subject><subject>Biological Transport, Active</subject><subject>Carnitine - pharmacokinetics</subject><subject>Carnitine - pharmacology</subject><subject>Epididymis</subject><subject>Fluorescent Antibody Technique</subject><subject>Male</subject><subject>Membrane Proteins - analysis</subject><subject>Membrane Proteins - antagonists & inhibitors</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Organic Cation Transport Proteins - analysis</subject><subject>Organic Cation Transport Proteins - antagonists & inhibitors</subject><subject>Organic Cation Transport Proteins - metabolism</subject><subject>Pyrilamine - pharmacology</subject><subject>Solute Carrier Family 22 Member 5</subject><subject>Sperm Maturation - physiology</subject><subject>Sperm Motility - physiology</subject><subject>Spermatozoa - metabolism</subject><issn>1470-1626</issn><issn>1741-7899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuLFDEQh4Mo7kNP3jUnUaTdPDpJ5yjD-oDFFZ09h3SSno10J22SQcbb_udm7FlGPHiqovjqR_EVAM8weosZRRdfL780iDcIC_oAnGLR4kZ0Uj6sfStQgznhJ-As5-8IYdYJ_hicYCE5I4ydgrt10iHPMRUYB2h0Cr744KAOFmrjym48zvrdEbiIaaODN3VSfAyw3Me4BF9dr9afX0PyJ2TfU-hDidDN3nq7m_QI8-zSpEv8FfUT8GjQY3ZPD_Uc3Ly_XK8-NlfXHz6t3l01fctZaWQnpDGYWiqM7evx1rEB9xxzgwiRpretZNINVFPNMDZdVx2QnktutCVI03PwcsmdU_yxdbmoyWfjxlEHF7dZ8a5taRVUwTcLaFLMOblBzclPOu0URmpvXFXjCnG1N17p54fYbT85e2QPiitAFuDWb25_-uRU72M23oXiB2_036n3f6xLL5alQUelN8lndfONIEwR6ggmnagEXoh_0v5362893qan</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Kobayashi, Daisuke</creator><creator>Tamai, Ikumi</creator><creator>Sai, Yoshimichi</creator><creator>Yoshida, Kazuhiro</creator><creator>Wakayama, Tomohiko</creator><creator>Kido, Yasuto</creator><creator>Nezu, Jun-ichi</creator><creator>Iseki, Shoichi</creator><creator>Tsuji, Akira</creator><general>Society for Reproduction and Fertility</general><general>BioScientifica Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa</title><author>Kobayashi, Daisuke ; Tamai, Ikumi ; Sai, Yoshimichi ; Yoshida, Kazuhiro ; Wakayama, Tomohiko ; Kido, Yasuto ; Nezu, Jun-ichi ; Iseki, Shoichi ; Tsuji, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b465t-9879cc13d37cdb525de5f1b616c0229cbd4959ef3a3a511c888992b696cad20a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acetylcarnitine - pharmacokinetics</topic><topic>Animals</topic><topic>Betaine - analogs & derivatives</topic><topic>Betaine - pharmacology</topic><topic>Biological Transport, Active</topic><topic>Carnitine - pharmacokinetics</topic><topic>Carnitine - pharmacology</topic><topic>Epididymis</topic><topic>Fluorescent Antibody Technique</topic><topic>Male</topic><topic>Membrane Proteins - analysis</topic><topic>Membrane Proteins - antagonists & inhibitors</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Organic Cation Transport Proteins - analysis</topic><topic>Organic Cation Transport Proteins - antagonists & inhibitors</topic><topic>Organic Cation Transport Proteins - metabolism</topic><topic>Pyrilamine - pharmacology</topic><topic>Solute Carrier Family 22 Member 5</topic><topic>Sperm Maturation - physiology</topic><topic>Sperm Motility - physiology</topic><topic>Spermatozoa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Daisuke</creatorcontrib><creatorcontrib>Tamai, Ikumi</creatorcontrib><creatorcontrib>Sai, Yoshimichi</creatorcontrib><creatorcontrib>Yoshida, Kazuhiro</creatorcontrib><creatorcontrib>Wakayama, Tomohiko</creatorcontrib><creatorcontrib>Kido, Yasuto</creatorcontrib><creatorcontrib>Nezu, Jun-ichi</creatorcontrib><creatorcontrib>Iseki, Shoichi</creatorcontrib><creatorcontrib>Tsuji, Akira</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Daisuke</au><au>Tamai, Ikumi</au><au>Sai, Yoshimichi</au><au>Yoshida, Kazuhiro</au><au>Wakayama, Tomohiko</au><au>Kido, Yasuto</au><au>Nezu, Jun-ichi</au><au>Iseki, Shoichi</au><au>Tsuji, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa</atitle><jtitle>Reproduction (Cambridge, England)</jtitle><addtitle>Reproduction</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>134</volume><issue>5</issue><spage>651</spage><epage>658</epage><pages>651-658</pages><issn>1470-1626</issn><eissn>1741-7899</eissn><abstract>Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na+-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with Km values of 23.6 and 6.57 μM for carnitine and acetylcarnitine respectively in the presence of Na+. Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na+-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.</abstract><cop>England</cop><pub>Society for Reproduction and Fertility</pub><pmid>17965255</pmid><doi>10.1530/REP-06-0173</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Acetylcarnitine - pharmacokinetics Animals Betaine - analogs & derivatives Betaine - pharmacology Biological Transport, Active Carnitine - pharmacokinetics Carnitine - pharmacology Epididymis Fluorescent Antibody Technique Male Membrane Proteins - analysis Membrane Proteins - antagonists & inhibitors Membrane Proteins - metabolism Mice Organic Cation Transport Proteins - analysis Organic Cation Transport Proteins - antagonists & inhibitors Organic Cation Transport Proteins - metabolism Pyrilamine - pharmacology Solute Carrier Family 22 Member 5 Sperm Maturation - physiology Sperm Motility - physiology Spermatozoa - metabolism |
title | Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa |
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