Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa

Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake...

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Veröffentlicht in:Reproduction (Cambridge, England) England), 2007-11, Vol.134 (5), p.651-658
Hauptverfasser: Kobayashi, Daisuke, Tamai, Ikumi, Sai, Yoshimichi, Yoshida, Kazuhiro, Wakayama, Tomohiko, Kido, Yasuto, Nezu, Jun-ichi, Iseki, Shoichi, Tsuji, Akira
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container_end_page 658
container_issue 5
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container_title Reproduction (Cambridge, England)
container_volume 134
creator Kobayashi, Daisuke
Tamai, Ikumi
Sai, Yoshimichi
Yoshida, Kazuhiro
Wakayama, Tomohiko
Kido, Yasuto
Nezu, Jun-ichi
Iseki, Shoichi
Tsuji, Akira
description Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na+-dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with Km values of 23.6 and 6.57 μM for carnitine and acetylcarnitine respectively in the presence of Na+. Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na+-dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.
doi_str_mv 10.1530/REP-06-0173
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subjects Acetylcarnitine - pharmacokinetics
Animals
Betaine - analogs & derivatives
Betaine - pharmacology
Biological Transport, Active
Carnitine - pharmacokinetics
Carnitine - pharmacology
Epididymis
Fluorescent Antibody Technique
Male
Membrane Proteins - analysis
Membrane Proteins - antagonists & inhibitors
Membrane Proteins - metabolism
Mice
Organic Cation Transport Proteins - analysis
Organic Cation Transport Proteins - antagonists & inhibitors
Organic Cation Transport Proteins - metabolism
Pyrilamine - pharmacology
Solute Carrier Family 22 Member 5
Sperm Maturation - physiology
Sperm Motility - physiology
Spermatozoa - metabolism
title Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa
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