Purification and molecular cloning of an intracellular 3-hydroxybutyrate-oligomer hydrolase from Paucimonas lemoignei

An intracellular 3-hydroxybutyrate-oligomer hydrolase was purified from a poly(3-hydroxybutyrate)-degrading bacterium, Paucimonas lemoignei. It hydrolyzed the 3-hydroxybutyrate dimer with the highest specific activity of any of the enzymes reported so far. The gene was cloned and sequenced. The dedu...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2007-09, Vol.104 (3), p.224-226
Hauptverfasser:	Uchino, Keiichi, Katsumata, Yoko, Takeda, Tomoko, Arai, Hiroki, Shiraki, Mari, Saito, Terumi
Format:	Artikel
Sprache:	eng
Schlagworte:	3-hydroxybutyrate-oligomer hydrolase 3-Hydroxybutyric Acid - chemistry AMINO ACID SEQUENCES BACTERIA Biological and medical sciences Biotechnology Burkholderia - enzymology Burkholderia - genetics CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular - methods Dimerization Enzyme Activation Enzyme Stability Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology gene cloning HIDROLASAS HYDROLASE HYDROLASES Hydrolases - chemistry Hydrolases - genetics MOLECULAR CLONING Paucimonas lemoignei PhaZc PURIFICACION PURIFICATION Ralstonia eutropha Recombinant Proteins - chemistry Recombinant Proteins - metabolism SECUENCIAS DE AMINOACIDOS SEQUENCE D'ACIDES AMINES Substrate Specificity Transfection - methods
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