Purification and characterization of rhodobactin: a mixed ligand siderophore from Rhodococcus rhodochrous strain OFS
The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D (1)H, (13)C and (15)N...
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| Veröffentlicht in: | Biometals 2007-12, Vol.20 (6), p.853-867 |
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| creator | Dhungana, Suraj Michalczyk, Ryszard Boukhalfa, Hakim Lack, Joseph G Koppisch, Andrew T Fairlee, Jason M Johnson, Mitchell T Ruggiero, Christy E John, Seth G Cox, Matthew M Browder, Cindy C Forsythe, Jennifer H Vanderberg, Laura A Neu, Mary P Hersman, Larry E |
| description | The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D (1)H, (13)C and (15)N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production and found that Fe limiting conditions (Fe concentrations from 0.1 microM to 2.0 microM) facilitated siderophore excretion. Our interests lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation. |
| doi_str_mv | 10.1007/s10534-006-9079-y |
| format | Article |
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The siderophore structure was characterized using 1D and 2D (1)H, (13)C and (15)N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production and found that Fe limiting conditions (Fe concentrations from 0.1 microM to 2.0 microM) facilitated siderophore excretion. Our interests lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation.</description><identifier>ISSN: 0966-0844</identifier><identifier>EISSN: 1572-8773</identifier><identifier>DOI: 10.1007/s10534-006-9079-y</identifier><identifier>PMID: 17273817</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Agar - chemistry ; Biophysics ; Bioremediation ; Chelation ; Chromatography, High Pressure Liquid - methods ; Epinephrine - analogs & derivatives ; Epinephrine - metabolism ; Hydrogen-Ion Concentration ; Hydrolysis ; Iron ; Iron - chemistry ; Iron - metabolism ; Ligands ; Liquid chromatography ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Metals ; Metals - chemistry ; Models, Chemical ; Peptides - chemistry ; Rhodococcus - metabolism ; Rhodococcus rhodochrous ; Siderophores - chemistry ; Spectrometry, Mass, Electrospray Ionization ; Spectrophotometry - methods ; Spectrophotometry, Ultraviolet - methods ; Time Factors</subject><ispartof>Biometals, 2007-12, Vol.20 (6), p.853-867</ispartof><rights>Springer Science+Business Media, LLC. 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-9c90fcfab00ea4da3d7ede92323f136a15d127f765b7d40105b9c8332a0dc2fa3</citedby><cites>FETCH-LOGICAL-c357t-9c90fcfab00ea4da3d7ede92323f136a15d127f765b7d40105b9c8332a0dc2fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17273817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dhungana, Suraj</creatorcontrib><creatorcontrib>Michalczyk, Ryszard</creatorcontrib><creatorcontrib>Boukhalfa, Hakim</creatorcontrib><creatorcontrib>Lack, Joseph G</creatorcontrib><creatorcontrib>Koppisch, Andrew T</creatorcontrib><creatorcontrib>Fairlee, Jason M</creatorcontrib><creatorcontrib>Johnson, Mitchell T</creatorcontrib><creatorcontrib>Ruggiero, Christy E</creatorcontrib><creatorcontrib>John, Seth G</creatorcontrib><creatorcontrib>Cox, Matthew M</creatorcontrib><creatorcontrib>Browder, Cindy C</creatorcontrib><creatorcontrib>Forsythe, Jennifer H</creatorcontrib><creatorcontrib>Vanderberg, Laura A</creatorcontrib><creatorcontrib>Neu, Mary P</creatorcontrib><creatorcontrib>Hersman, Larry E</creatorcontrib><title>Purification and characterization of rhodobactin: a mixed ligand siderophore from Rhodococcus rhodochrous strain OFS</title><title>Biometals</title><addtitle>Biometals</addtitle><description>The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D (1)H, (13)C and (15)N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production and found that Fe limiting conditions (Fe concentrations from 0.1 microM to 2.0 microM) facilitated siderophore excretion. Our interests lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation.</description><subject>Agar - chemistry</subject><subject>Biophysics</subject><subject>Bioremediation</subject><subject>Chelation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Epinephrine - analogs & derivatives</subject><subject>Epinephrine - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Iron</subject><subject>Iron - chemistry</subject><subject>Iron - metabolism</subject><subject>Ligands</subject><subject>Liquid chromatography</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mass Spectrometry</subject><subject>Metals</subject><subject>Metals - chemistry</subject><subject>Models, Chemical</subject><subject>Peptides - chemistry</subject><subject>Rhodococcus - 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The siderophore structure was characterized using 1D and 2D (1)H, (13)C and (15)N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production and found that Fe limiting conditions (Fe concentrations from 0.1 microM to 2.0 microM) facilitated siderophore excretion. Our interests lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>17273817</pmid><doi>10.1007/s10534-006-9079-y</doi><tpages>15</tpages></addata></record> |
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| ispartof | Biometals, 2007-12, Vol.20 (6), p.853-867 |
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| subjects | Agar - chemistry Biophysics Bioremediation Chelation Chromatography, High Pressure Liquid - methods Epinephrine - analogs & derivatives Epinephrine - metabolism Hydrogen-Ion Concentration Hydrolysis Iron Iron - chemistry Iron - metabolism Ligands Liquid chromatography Magnetic Resonance Spectroscopy Mass Spectrometry Metals Metals - chemistry Models, Chemical Peptides - chemistry Rhodococcus - metabolism Rhodococcus rhodochrous Siderophores - chemistry Spectrometry, Mass, Electrospray Ionization Spectrophotometry - methods Spectrophotometry, Ultraviolet - methods Time Factors |
| title | Purification and characterization of rhodobactin: a mixed ligand siderophore from Rhodococcus rhodochrous strain OFS |
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