Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies
Tissue engineering is a promising approach to developing hepatic tissue suitable for the functional replacement of a failing liver. The aim of the present study was to investigate whether an extracellular cell matrix obtained from fibroblasts-cultured within scaffolds of hyaluronic acid (HYAFF™) cou...
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| Veröffentlicht in: | Biomaterials 2005-12, Vol.26 (34), p.7038-7045 |
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| container_title | Biomaterials |
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| creator | Zavan, B. Brun, P. Vindigni, V. Amadori, A. Habeler, W. Pontisso, P. Montemurro, D. Abatangelo, G. Cortivo, R. |
| description | Tissue engineering is a promising approach to developing hepatic tissue suitable for the functional replacement of a failing liver. The aim of the present study was to investigate whether an extracellular cell matrix obtained from fibroblasts-cultured within scaffolds of hyaluronic acid (HYAFF™) could influence the proliferation rate and survival of rat hepatocytes both during long-term culture and after in vivo transplantation. Cultures were evaluated by histological and morphological analysis, a proliferation assay and metabolic activity (albumin secretion). Hepatocytes cultured in extracellular matrix-enriched scaffolds exhibited a round cellular morphology and re-established cell–cell contacts, growing into aggregates of several cells along and/or among fibers in the fabric. Hepatocytes were able to secrete albumin up to 14 days in culture. In vivo results demonstrated the biocompatibility of HYAFF-11
TM implanted in nude mice, in which hepatocytes maintained small well-organised aggregates until the 35th day.
In conclusion, the presence of a fibroblast-secreted extracellular matrix improved the biological properties of the hyaluronan scaffold, favoring the survival and morphological integrity of hepatocytes in vitro and in vivo. |
| doi_str_mv | 10.1016/j.biomaterials.2005.04.067 |
| format | Article |
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TM implanted in nude mice, in which hepatocytes maintained small well-organised aggregates until the 35th day.
In conclusion, the presence of a fibroblast-secreted extracellular matrix improved the biological properties of the hyaluronan scaffold, favoring the survival and morphological integrity of hepatocytes in vitro and in vivo.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2005.04.067</identifier><identifier>PMID: 15993941</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Biocompatible Materials - chemistry ; Biomaterial ; Cell culture ; Cell Culture Techniques - methods ; Cell morphology ; Cell Proliferation ; Cell Survival - physiology ; Cells, Cultured ; Extracellular Matrix - metabolism ; Fibroblast ; Fibroblasts - metabolism ; Hepatocyte ; Hepatocytes - cytology ; Hepatocytes - physiology ; Hepatocytes - transplantation ; Humans ; Hyaluronic acid ; Hyaluronic Acid - chemistry ; Liver, Artificial ; Materials Testing ; Mice ; Mice, SCID ; Rats ; Rats, Wistar ; Scaffold ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2005-12, Vol.26 (34), p.7038-7045</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-a24db1f36071ba58acdf959eaa17366099766a7c4e8953e0617bff41dafaee33</citedby><cites>FETCH-LOGICAL-c506t-a24db1f36071ba58acdf959eaa17366099766a7c4e8953e0617bff41dafaee33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2005.04.067$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15993941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zavan, B.</creatorcontrib><creatorcontrib>Brun, P.</creatorcontrib><creatorcontrib>Vindigni, V.</creatorcontrib><creatorcontrib>Amadori, A.</creatorcontrib><creatorcontrib>Habeler, W.</creatorcontrib><creatorcontrib>Pontisso, P.</creatorcontrib><creatorcontrib>Montemurro, D.</creatorcontrib><creatorcontrib>Abatangelo, G.</creatorcontrib><creatorcontrib>Cortivo, R.</creatorcontrib><title>Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Tissue engineering is a promising approach to developing hepatic tissue suitable for the functional replacement of a failing liver. The aim of the present study was to investigate whether an extracellular cell matrix obtained from fibroblasts-cultured within scaffolds of hyaluronic acid (HYAFF™) could influence the proliferation rate and survival of rat hepatocytes both during long-term culture and after in vivo transplantation. Cultures were evaluated by histological and morphological analysis, a proliferation assay and metabolic activity (albumin secretion). Hepatocytes cultured in extracellular matrix-enriched scaffolds exhibited a round cellular morphology and re-established cell–cell contacts, growing into aggregates of several cells along and/or among fibers in the fabric. Hepatocytes were able to secrete albumin up to 14 days in culture. In vivo results demonstrated the biocompatibility of HYAFF-11
TM implanted in nude mice, in which hepatocytes maintained small well-organised aggregates until the 35th day.
In conclusion, the presence of a fibroblast-secreted extracellular matrix improved the biological properties of the hyaluronan scaffold, favoring the survival and morphological integrity of hepatocytes in vitro and in vivo.</description><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biomaterial</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell morphology</subject><subject>Cell Proliferation</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fibroblast</subject><subject>Fibroblasts - metabolism</subject><subject>Hepatocyte</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - physiology</subject><subject>Hepatocytes - transplantation</subject><subject>Humans</subject><subject>Hyaluronic acid</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Liver, Artificial</subject><subject>Materials Testing</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Scaffold</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtr3DAUhUVoSKZp_0IRXXRnV7L1sLIrafqAQDbZC1m6IhpsayrJQ-bfR8MMtLsUBOLAd-650kHoMyUtJVR83bZjiLMpkIKZctsRwlvCWiLkBdrQQQ4NV4S_QxtCWdcoQbtr9D7nLamasO4KXVOuVK8Y3aD9_UtJxsI0rZNJuE5N4aWBJQX7DA7v4nSYa47F2Rrv4-QyNvXgvI65GgtgHxN-hp0p0R6qtOtU1gT5FocF70NJEZvFncQ-4lxWFyB_QJe-7g4fz_cNevpx_3T3q3l4_Pn77ttDYzkRpTEdcyP1vSCSjoYPxjqvuAJjqOyFIEpJIYy0DAbFeyCCytF7Rp3xBqDvb9CX09hdin9WyEXPIR8faxaIa9ZiYD0fhHgT7AbRUSnZmyBVrBO9IhW8PYE2xZwTeL1LYTbpoCnRxxr1Vv9boz7WqAnTtcZq_nROWccZ3F_rubcKfD8BUD9vHyDpbAMsFlxIYIt2MfxPzitO97ha</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Zavan, B.</creator><creator>Brun, P.</creator><creator>Vindigni, V.</creator><creator>Amadori, A.</creator><creator>Habeler, W.</creator><creator>Pontisso, P.</creator><creator>Montemurro, D.</creator><creator>Abatangelo, G.</creator><creator>Cortivo, R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies</title><author>Zavan, B. ; Brun, P. ; Vindigni, V. ; Amadori, A. ; Habeler, W. ; Pontisso, P. ; Montemurro, D. ; Abatangelo, G. ; Cortivo, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-a24db1f36071ba58acdf959eaa17366099766a7c4e8953e0617bff41dafaee33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biomaterial</topic><topic>Cell culture</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell morphology</topic><topic>Cell Proliferation</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fibroblast</topic><topic>Fibroblasts - metabolism</topic><topic>Hepatocyte</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - physiology</topic><topic>Hepatocytes - transplantation</topic><topic>Humans</topic><topic>Hyaluronic acid</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Liver, Artificial</topic><topic>Materials Testing</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Scaffold</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zavan, B.</creatorcontrib><creatorcontrib>Brun, P.</creatorcontrib><creatorcontrib>Vindigni, V.</creatorcontrib><creatorcontrib>Amadori, A.</creatorcontrib><creatorcontrib>Habeler, W.</creatorcontrib><creatorcontrib>Pontisso, P.</creatorcontrib><creatorcontrib>Montemurro, D.</creatorcontrib><creatorcontrib>Abatangelo, G.</creatorcontrib><creatorcontrib>Cortivo, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zavan, B.</au><au>Brun, P.</au><au>Vindigni, V.</au><au>Amadori, A.</au><au>Habeler, W.</au><au>Pontisso, P.</au><au>Montemurro, D.</au><au>Abatangelo, G.</au><au>Cortivo, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>26</volume><issue>34</issue><spage>7038</spage><epage>7045</epage><pages>7038-7045</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Tissue engineering is a promising approach to developing hepatic tissue suitable for the functional replacement of a failing liver. The aim of the present study was to investigate whether an extracellular cell matrix obtained from fibroblasts-cultured within scaffolds of hyaluronic acid (HYAFF™) could influence the proliferation rate and survival of rat hepatocytes both during long-term culture and after in vivo transplantation. Cultures were evaluated by histological and morphological analysis, a proliferation assay and metabolic activity (albumin secretion). Hepatocytes cultured in extracellular matrix-enriched scaffolds exhibited a round cellular morphology and re-established cell–cell contacts, growing into aggregates of several cells along and/or among fibers in the fabric. Hepatocytes were able to secrete albumin up to 14 days in culture. In vivo results demonstrated the biocompatibility of HYAFF-11
TM implanted in nude mice, in which hepatocytes maintained small well-organised aggregates until the 35th day.
In conclusion, the presence of a fibroblast-secreted extracellular matrix improved the biological properties of the hyaluronan scaffold, favoring the survival and morphological integrity of hepatocytes in vitro and in vivo.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>15993941</pmid><doi>10.1016/j.biomaterials.2005.04.067</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biocompatible Materials - chemistry Biomaterial Cell culture Cell Culture Techniques - methods Cell morphology Cell Proliferation Cell Survival - physiology Cells, Cultured Extracellular Matrix - metabolism Fibroblast Fibroblasts - metabolism Hepatocyte Hepatocytes - cytology Hepatocytes - physiology Hepatocytes - transplantation Humans Hyaluronic acid Hyaluronic Acid - chemistry Liver, Artificial Materials Testing Mice Mice, SCID Rats Rats, Wistar Scaffold Tissue Engineering - methods |
| title | Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies |
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