Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat
N₂ fixation, diazotrophic community composition, and organisms actively expressing genes for N₂ fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA). Specifi...
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| Veröffentlicht in: | Microbial ecology 2005-02, Vol.49 (2), p.315-324 |
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| creator | Steppe, T. F. Paerl, H. W. |
| description | N₂ fixation, diazotrophic community composition, and organisms actively expressing genes for N₂ fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA). Specifically, diel variations of N₂ fixation in the mats from the RCNERR were examined. Three distinct diel patterns of nitrogenase activity (NA) were observed. NA responses to short-term inhibitions of photosynthesis corresponded to one of the three patterns. High rates of NA were observed during peak O₂ production periods for diel experiments during summer months. Different types of NA diel variations correspond to different stages of mat development. Chloramphenicol treatments indicated that the mechanism of protein synthesis supporting NA changed throughout the day. Analysis of mat DNA and RNA gave further evidence suggesting that in addition to cyanobacteria, other functional groups were responsible for the NA observed in the RCNERR mats. The role of microbial diversity in the N₂ fixation dynamics of these mats is discussed. |
| doi_str_mv | 10.1007/s00248-004-0245-x |
| format | Article |
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F. ; Paerl, H. W.</creator><creatorcontrib>Steppe, T. F. ; Paerl, H. W.</creatorcontrib><description>N₂ fixation, diazotrophic community composition, and organisms actively expressing genes for N₂ fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA). Specifically, diel variations of N₂ fixation in the mats from the RCNERR were examined. Three distinct diel patterns of nitrogenase activity (NA) were observed. NA responses to short-term inhibitions of photosynthesis corresponded to one of the three patterns. High rates of NA were observed during peak O₂ production periods for diel experiments during summer months. Different types of NA diel variations correspond to different stages of mat development. Chloramphenicol treatments indicated that the mechanism of protein synthesis supporting NA changed throughout the day. Analysis of mat DNA and RNA gave further evidence suggesting that in addition to cyanobacteria, other functional groups were responsible for the NA observed in the RCNERR mats. The role of microbial diversity in the N₂ fixation dynamics of these mats is discussed.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-004-0245-x</identifier><identifier>PMID: 15970996</identifier><identifier>CODEN: MCBEBU</identifier><language>eng</language><publisher>New York, NY: Springer Science + Business Media, Inc</publisher><subject>Bacteria ; Bacteria - enzymology ; Bacteriology ; Biodiversity ; Biological and medical sciences ; Circadian Rhythm ; Community composition ; Cyanobacteria ; Diel variations ; DNA ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Lyngbya ; Microbiology ; Miscellaneous ; Nitrogen fixation ; Nitrogenase - metabolism ; Oxidoreductases - metabolism ; Photosynthesis ; Phylogeny ; Polymerase chain reaction ; Protein synthesis ; Reverse transcriptase polymerase chain reaction ; RNA ; Seawater - microbiology ; Steppes</subject><ispartof>Microbial ecology, 2005-02, Vol.49 (2), p.315-324</ispartof><rights>Copyright 2005 Springer Science+Business Media, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>Springer Science+Business Media, Inc. 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-389b17cf714168bab8241232151e5da10ab38d98b1d70edaa30696e0ce81931d3</citedby><cites>FETCH-LOGICAL-c409t-389b17cf714168bab8241232151e5da10ab38d98b1d70edaa30696e0ce81931d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25153181$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25153181$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17004108$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15970996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Steppe, T. F.</creatorcontrib><creatorcontrib>Paerl, H. W.</creatorcontrib><title>Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><description>N₂ fixation, diazotrophic community composition, and organisms actively expressing genes for N₂ fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA). Specifically, diel variations of N₂ fixation in the mats from the RCNERR were examined. Three distinct diel patterns of nitrogenase activity (NA) were observed. NA responses to short-term inhibitions of photosynthesis corresponded to one of the three patterns. High rates of NA were observed during peak O₂ production periods for diel experiments during summer months. Different types of NA diel variations correspond to different stages of mat development. Chloramphenicol treatments indicated that the mechanism of protein synthesis supporting NA changed throughout the day. Analysis of mat DNA and RNA gave further evidence suggesting that in addition to cyanobacteria, other functional groups were responsible for the NA observed in the RCNERR mats. The role of microbial diversity in the N₂ fixation dynamics of these mats is discussed.</description><subject>Bacteria</subject><subject>Bacteria - enzymology</subject><subject>Bacteriology</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>Circadian Rhythm</subject><subject>Community composition</subject><subject>Cyanobacteria</subject><subject>Diel variations</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Lyngbya</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Nitrogen fixation</subject><subject>Nitrogenase - metabolism</subject><subject>Oxidoreductases - metabolism</subject><subject>Photosynthesis</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Protein synthesis</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>RNA</subject><subject>Seawater - microbiology</subject><subject>Steppes</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU1rGzEQhkVoSVy3PyCHFhFob5vM6GNXOoaQNoG4uaTQm9DuaoPMWutKcnD-fWRsGsglpxmYZ16YeQg5RThHgOYiATChKgBRlUZW2yMyQ8FZhUr8_UBmAFpWvGbqhHxKaQmATc34MTlBqRvQup6RxW-f4_Togk2OXnbZP_n8TG3oafDDDb3erqNLyU-B-kAtXdjog6O3IbuYfW9HuvBdnFq_62z-TD4Odkzuy6HOyZ-f1w9XN9Xd_a_bq8u7qhOgc8WVbrHphgYF1qq1rWICGWco0cneItiWq16rFvsGXG8th1rXDjqnUHPs-Zz82Oeu4_Rv41I2K586N442uGmTTK0E00Lrd0HUWkrN6gKevQGX0yaGcoRRDBEbVT43J7iHyskpRTeYdfQrG58NgtkZMXsjphgxOyNmW3a-HYI37cr1rxsHBQX4fgBs6uw4RBs6n165poQhqMJ93XPLlKf4f84kSo4K-QuwLJth</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Steppe, T. 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F. ; Paerl, H. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-389b17cf714168bab8241232151e5da10ab38d98b1d70edaa30696e0ce81931d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacteria</topic><topic>Bacteria - enzymology</topic><topic>Bacteriology</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>Circadian Rhythm</topic><topic>Community composition</topic><topic>Cyanobacteria</topic><topic>Diel variations</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. 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F.</au><au>Paerl, H. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat</atitle><jtitle>Microbial ecology</jtitle><addtitle>Microb Ecol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>49</volume><issue>2</issue><spage>315</spage><epage>324</epage><pages>315-324</pages><issn>0095-3628</issn><eissn>1432-184X</eissn><coden>MCBEBU</coden><abstract>N₂ fixation, diazotrophic community composition, and organisms actively expressing genes for N₂ fixation were examined over at 3-year period (1997-1999) for intertidal microbial mats on a sand flat located in the Rachel Carson National Estuarine Research Reserve (RCNERR) (Beaufort, NC, USA). Specifically, diel variations of N₂ fixation in the mats from the RCNERR were examined. Three distinct diel patterns of nitrogenase activity (NA) were observed. NA responses to short-term inhibitions of photosynthesis corresponded to one of the three patterns. High rates of NA were observed during peak O₂ production periods for diel experiments during summer months. Different types of NA diel variations correspond to different stages of mat development. Chloramphenicol treatments indicated that the mechanism of protein synthesis supporting NA changed throughout the day. Analysis of mat DNA and RNA gave further evidence suggesting that in addition to cyanobacteria, other functional groups were responsible for the NA observed in the RCNERR mats. The role of microbial diversity in the N₂ fixation dynamics of these mats is discussed.</abstract><cop>New York, NY</cop><pub>Springer Science + Business Media, Inc</pub><pmid>15970996</pmid><doi>10.1007/s00248-004-0245-x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Microbial ecology, 2005-02, Vol.49 (2), p.315-324 |
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| source | Jstor Complete Legacy; MEDLINE; Springer Journals |
| subjects | Bacteria Bacteria - enzymology Bacteriology Biodiversity Biological and medical sciences Circadian Rhythm Community composition Cyanobacteria Diel variations DNA Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Lyngbya Microbiology Miscellaneous Nitrogen fixation Nitrogenase - metabolism Oxidoreductases - metabolism Photosynthesis Phylogeny Polymerase chain reaction Protein synthesis Reverse transcriptase polymerase chain reaction RNA Seawater - microbiology Steppes |
| title | Nitrogenase Activity and nifH Expression in a Marine Intertidal Microbial Mat |
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