Attachment of infectious influenza A viruses of various subtypes to live mammalian and avian cells as measured by flow cytometry
At present there is much interest in the cell tropism and host range of influenza viruses, especially those of the H5N1 subtype. We wished to develop a method that would enable investigation of attachment of infectious virus through the interaction of the hemagglutinin molecule and live mammalian an...
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| Veröffentlicht in: | Virus research 2007-11, Vol.129 (2), p.175-181 |
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| creator | Rimmelzwaan, Guus F. Nieuwkoop, Nella J. de Mutsert, Gerrie Boon, Adrianus C.M. Kuiken, Thijs Fouchier, Ron A.M. Osterhaus, Albert D.M.E. |
| description | At present there is much interest in the cell tropism and host range of influenza viruses, especially those of the H5N1 subtype. We wished to develop a method that would enable investigation of attachment of infectious virus through the interaction of the hemagglutinin molecule and live mammalian and avian cells and the subsequent infection of these cells. To this end, influenza viruses of various HA subtypes were constructed that either carry the green fluorescent protein (GFP) instead of the neuraminidase protein, or that express GFP in the cytoplasm of infected cells. The HA genes were derived from influenza viruses A/PR/8/34 (H1N1), A/Netherlands/178/95 (H3N2) and A/Vietnam/1194/04 (H5N1). Using these pairs of viruses, attachment and post-attachment events in the virus replication cycle can be distinguished. In general, the expression of NeuAc(α2-3)Gal or NeuAc(α2-6)Gal receptors on the cells tested corresponded with the attachment of the viruses that were studied with respect to predicted receptor specificity. Virus attachment was not always predictive for efficient infection of the cells. |
| doi_str_mv | 10.1016/j.virusres.2007.07.007 |
| format | Article |
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We wished to develop a method that would enable investigation of attachment of infectious virus through the interaction of the hemagglutinin molecule and live mammalian and avian cells and the subsequent infection of these cells. To this end, influenza viruses of various HA subtypes were constructed that either carry the green fluorescent protein (GFP) instead of the neuraminidase protein, or that express GFP in the cytoplasm of infected cells. The HA genes were derived from influenza viruses A/PR/8/34 (H1N1), A/Netherlands/178/95 (H3N2) and A/Vietnam/1194/04 (H5N1). Using these pairs of viruses, attachment and post-attachment events in the virus replication cycle can be distinguished. In general, the expression of NeuAc(α2-3)Gal or NeuAc(α2-6)Gal receptors on the cells tested corresponded with the attachment of the viruses that were studied with respect to predicted receptor specificity. 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Virus attachment was not always predictive for efficient infection of the cells.</description><subject>Animals</subject><subject>Binding</subject><subject>Birds - virology</subject><subject>Cell Line</subject><subject>Flow Cytometry</subject><subject>Green fluorescent protein</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - genetics</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - metabolism</subject><subject>Humans</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza A virus - physiology</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza A Virus, H1N1 Subtype - isolation & purification</subject><subject>Influenza A Virus, H1N1 Subtype - physiology</subject><subject>Influenza A Virus, H3N2 Subtype - genetics</subject><subject>Influenza A Virus, H3N2 Subtype - isolation & purification</subject><subject>Influenza A Virus, H3N2 Subtype - physiology</subject><subject>Influenza A Virus, H5N1 Subtype - genetics</subject><subject>Influenza A Virus, H5N1 Subtype - isolation & purification</subject><subject>Influenza A Virus, H5N1 Subtype - physiology</subject><subject>Influenza virus</subject><subject>Orthomyxoviridae Infections - virology</subject><subject>Receptor</subject><subject>Receptors, Virus - metabolism</subject><subject>Virus Attachment</subject><subject>Virus Replication</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO3DAQhq2KqizQV0A-9ZZlnE3scOsKQYuExIWeLceeCK_iZGs7qcKJR8dmt-KINJJHM__M7_kIuWSwZsD41W49Wz8Fj2FdAoh1DhBfyIo1oixEdV2ekFUSNgUTUJ6SsxB2AMA3gn8jp0wIVjUlrMjrNkalnx0OkY4dtUOHOtpxCjntJxxeFN3Sdy8MWTEr_94OUxuXfarFkfZ2RuqUc6q3aqBqMFTNOdPY94GqQB2qMHk0tF1o14__qF7i6DD65YJ87VQf8PvxPSd_7m6fbn4XD4-_7m-2D4XecB4LbUA1tdmAgEpxbDVURjQaocJUwbZsa614ZTo0OrVrBVCbrtU1u-ZtybrNOflx2Lv3498JQ5TOhvw_NWC6R_KmKhMVkYT8INR-DAlwJ_feOuUXyUBm9nIn_7OXmb3MAXnw8ugwtQ7Nx9gRdhL8PAgw3Tlb9DJoi4NGY32iLs1oP_N4A-3bnYg</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Rimmelzwaan, Guus F.</creator><creator>Nieuwkoop, Nella J.</creator><creator>de Mutsert, Gerrie</creator><creator>Boon, Adrianus C.M.</creator><creator>Kuiken, Thijs</creator><creator>Fouchier, Ron A.M.</creator><creator>Osterhaus, Albert D.M.E.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Attachment of infectious influenza A viruses of various subtypes to live mammalian and avian cells as measured by flow cytometry</title><author>Rimmelzwaan, Guus F. ; 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We wished to develop a method that would enable investigation of attachment of infectious virus through the interaction of the hemagglutinin molecule and live mammalian and avian cells and the subsequent infection of these cells. To this end, influenza viruses of various HA subtypes were constructed that either carry the green fluorescent protein (GFP) instead of the neuraminidase protein, or that express GFP in the cytoplasm of infected cells. The HA genes were derived from influenza viruses A/PR/8/34 (H1N1), A/Netherlands/178/95 (H3N2) and A/Vietnam/1194/04 (H5N1). Using these pairs of viruses, attachment and post-attachment events in the virus replication cycle can be distinguished. In general, the expression of NeuAc(α2-3)Gal or NeuAc(α2-6)Gal receptors on the cells tested corresponded with the attachment of the viruses that were studied with respect to predicted receptor specificity. 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| fulltext | fulltext |
| identifier | ISSN: 0168-1702 |
| ispartof | Virus research, 2007-11, Vol.129 (2), p.175-181 |
| issn | 0168-1702 1872-7492 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Binding Birds - virology Cell Line Flow Cytometry Green fluorescent protein Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - metabolism Humans Influenza A virus - genetics Influenza A virus - isolation & purification Influenza A virus - physiology Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - isolation & purification Influenza A Virus, H1N1 Subtype - physiology Influenza A Virus, H3N2 Subtype - genetics Influenza A Virus, H3N2 Subtype - isolation & purification Influenza A Virus, H3N2 Subtype - physiology Influenza A Virus, H5N1 Subtype - genetics Influenza A Virus, H5N1 Subtype - isolation & purification Influenza A Virus, H5N1 Subtype - physiology Influenza virus Orthomyxoviridae Infections - virology Receptor Receptors, Virus - metabolism Virus Attachment Virus Replication |
| title | Attachment of infectious influenza A viruses of various subtypes to live mammalian and avian cells as measured by flow cytometry |
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