Human endothelial cell growth and phenotypic expression on three dimensional poly(lactide-co-glycolide) sintered microsphere scaffolds for bone tissue engineering
Bone tissue engineering offers promising alternatives to repair and restore tissues. Our laboratory has employed poly(lactid-co-glycolide) PLAGA microspheres to develop a three dimensional (3-D) porous bioresorbable scaffold with a biomimetic pore structure. Osseous healing and integration with the...
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| Veröffentlicht in: | Biotechnology and bioengineering 2007-12, Vol.98 (5), p.1094-1102 |
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| creator | Jabbarzadeh, Ehsan Jiang, Tao Deng, Meng Nair, Lakshmi S Khan, Yusuf M Laurencin, Cato T |
| description | Bone tissue engineering offers promising alternatives to repair and restore tissues. Our laboratory has employed poly(lactid-co-glycolide) PLAGA microspheres to develop a three dimensional (3-D) porous bioresorbable scaffold with a biomimetic pore structure. Osseous healing and integration with the surrounding tissue depends in part on new blood vessel formation within the porous structure. Since endothelial cells play a key role in angiogenesis (formation of new blood vessels from pre-existing vasculature), the purpose of this study was to better understand human endothelial cell attachment, viability, growth, and phenotypic expression on sintered PLAGA microsphere scaffold. Scanning electron microscopy (SEM) examination showed cells attaching to the surface of microspheres and bridging the pores between the microspheres. Cell proliferation studies indicated that cell number increased during early stages and reached a plateau between days 10 and 14. Immunofluorescent staining for actin showed that cells were proliferating three dimensionally through the scaffolds while staining for PECAM-1 (platelet endothelial cell adhesion molecule) displayed typical localization at cell-cell contacts. Gene expression analysis showed that endothelial cells grown on PLAGA scaffolds maintained their normal characteristic phenotype. The cell proliferation and phenotypic expression were independent of scaffold pore architecture. These results demonstrate that PLAGA sintered microsphere scaffolds can support the growth and biological functions of human endothelial cells. The insights from this study should aid future studies aimed at enhancing angiogenesis in three dimensional tissue engineered scaffolds. Biotechnol. Bioeng. 2007;98: 1094-1102. © 2007 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.21495 |
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Our laboratory has employed poly(lactid-co-glycolide) PLAGA microspheres to develop a three dimensional (3-D) porous bioresorbable scaffold with a biomimetic pore structure. Osseous healing and integration with the surrounding tissue depends in part on new blood vessel formation within the porous structure. Since endothelial cells play a key role in angiogenesis (formation of new blood vessels from pre-existing vasculature), the purpose of this study was to better understand human endothelial cell attachment, viability, growth, and phenotypic expression on sintered PLAGA microsphere scaffold. Scanning electron microscopy (SEM) examination showed cells attaching to the surface of microspheres and bridging the pores between the microspheres. Cell proliferation studies indicated that cell number increased during early stages and reached a plateau between days 10 and 14. Immunofluorescent staining for actin showed that cells were proliferating three dimensionally through the scaffolds while staining for PECAM-1 (platelet endothelial cell adhesion molecule) displayed typical localization at cell-cell contacts. Gene expression analysis showed that endothelial cells grown on PLAGA scaffolds maintained their normal characteristic phenotype. The cell proliferation and phenotypic expression were independent of scaffold pore architecture. These results demonstrate that PLAGA sintered microsphere scaffolds can support the growth and biological functions of human endothelial cells. The insights from this study should aid future studies aimed at enhancing angiogenesis in three dimensional tissue engineered scaffolds. Biotechnol. Bioeng. 2007;98: 1094-1102. © 2007 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.21495</identifier><identifier>PMID: 17497742</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Actins - analysis ; angiogenesis ; Biological and medical sciences ; Biotechnology ; Blood vessels ; bone ; Bone and Bones - cytology ; Bone and Bones - metabolism ; Bone and Bones - physiology ; Bones ; Cell Adhesion ; Cell growth ; Cell Proliferation ; Cell Survival ; E-Selectin - genetics ; endothelial cell ; Endothelial Cells - chemistry ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genotype & phenotype ; Health. Pharmaceutical industry ; Humans ; Industrial applications and implications. Economical aspects ; Intercellular Adhesion Molecule-1 - genetics ; Microscopy, Electron, Scanning ; Microspheres ; Miscellaneous ; Platelet Endothelial Cell Adhesion Molecule-1 - analysis ; poly(lactide-co-glycolide) ; Polyglactin 910 - chemistry ; Polystyrenes - chemistry ; Studies ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds ; Umbilical Veins - cytology ; von Willebrand Factor - genetics</subject><ispartof>Biotechnology and bioengineering, 2007-12, Vol.98 (5), p.1094-1102</ispartof><rights>Copyright © 2007 Wiley Periodicals, Inc.</rights><rights>2008 INIST-CNRS</rights><rights>Copyright John Wiley and Sons, Limited Dec 1, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5045-3eac9580e71e133e09cfce4848a1646c875c97a6e4a4a343d4b75f2715849e693</citedby><cites>FETCH-LOGICAL-c5045-3eac9580e71e133e09cfce4848a1646c875c97a6e4a4a343d4b75f2715849e693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.21495$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.21495$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19237358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17497742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jabbarzadeh, Ehsan</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>Deng, Meng</creatorcontrib><creatorcontrib>Nair, Lakshmi S</creatorcontrib><creatorcontrib>Khan, Yusuf M</creatorcontrib><creatorcontrib>Laurencin, Cato T</creatorcontrib><title>Human endothelial cell growth and phenotypic expression on three dimensional poly(lactide-co-glycolide) sintered microsphere scaffolds for bone tissue engineering</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Bone tissue engineering offers promising alternatives to repair and restore tissues. Our laboratory has employed poly(lactid-co-glycolide) PLAGA microspheres to develop a three dimensional (3-D) porous bioresorbable scaffold with a biomimetic pore structure. Osseous healing and integration with the surrounding tissue depends in part on new blood vessel formation within the porous structure. Since endothelial cells play a key role in angiogenesis (formation of new blood vessels from pre-existing vasculature), the purpose of this study was to better understand human endothelial cell attachment, viability, growth, and phenotypic expression on sintered PLAGA microsphere scaffold. Scanning electron microscopy (SEM) examination showed cells attaching to the surface of microspheres and bridging the pores between the microspheres. Cell proliferation studies indicated that cell number increased during early stages and reached a plateau between days 10 and 14. Immunofluorescent staining for actin showed that cells were proliferating three dimensionally through the scaffolds while staining for PECAM-1 (platelet endothelial cell adhesion molecule) displayed typical localization at cell-cell contacts. Gene expression analysis showed that endothelial cells grown on PLAGA scaffolds maintained their normal characteristic phenotype. The cell proliferation and phenotypic expression were independent of scaffold pore architecture. These results demonstrate that PLAGA sintered microsphere scaffolds can support the growth and biological functions of human endothelial cells. The insights from this study should aid future studies aimed at enhancing angiogenesis in three dimensional tissue engineered scaffolds. Biotechnol. Bioeng. 2007;98: 1094-1102. © 2007 Wiley Periodicals, Inc.</description><subject>Actins - analysis</subject><subject>angiogenesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blood vessels</subject><subject>bone</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - metabolism</subject><subject>Bone and Bones - physiology</subject><subject>Bones</subject><subject>Cell Adhesion</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>E-Selectin - genetics</subject><subject>endothelial cell</subject><subject>Endothelial Cells - chemistry</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genotype & phenotype</subject><subject>Health. 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Psychology</topic><topic>Gene Expression</topic><topic>Genotype & phenotype</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Industrial applications and implications. 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Bioeng</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>98</volume><issue>5</issue><spage>1094</spage><epage>1102</epage><pages>1094-1102</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>Bone tissue engineering offers promising alternatives to repair and restore tissues. Our laboratory has employed poly(lactid-co-glycolide) PLAGA microspheres to develop a three dimensional (3-D) porous bioresorbable scaffold with a biomimetic pore structure. Osseous healing and integration with the surrounding tissue depends in part on new blood vessel formation within the porous structure. Since endothelial cells play a key role in angiogenesis (formation of new blood vessels from pre-existing vasculature), the purpose of this study was to better understand human endothelial cell attachment, viability, growth, and phenotypic expression on sintered PLAGA microsphere scaffold. Scanning electron microscopy (SEM) examination showed cells attaching to the surface of microspheres and bridging the pores between the microspheres. Cell proliferation studies indicated that cell number increased during early stages and reached a plateau between days 10 and 14. Immunofluorescent staining for actin showed that cells were proliferating three dimensionally through the scaffolds while staining for PECAM-1 (platelet endothelial cell adhesion molecule) displayed typical localization at cell-cell contacts. Gene expression analysis showed that endothelial cells grown on PLAGA scaffolds maintained their normal characteristic phenotype. The cell proliferation and phenotypic expression were independent of scaffold pore architecture. These results demonstrate that PLAGA sintered microsphere scaffolds can support the growth and biological functions of human endothelial cells. The insights from this study should aid future studies aimed at enhancing angiogenesis in three dimensional tissue engineered scaffolds. Biotechnol. Bioeng. 2007;98: 1094-1102. © 2007 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17497742</pmid><doi>10.1002/bit.21495</doi><tpages>9</tpages></addata></record> |
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| subjects | Actins - analysis angiogenesis Biological and medical sciences Biotechnology Blood vessels bone Bone and Bones - cytology Bone and Bones - metabolism Bone and Bones - physiology Bones Cell Adhesion Cell growth Cell Proliferation Cell Survival E-Selectin - genetics endothelial cell Endothelial Cells - chemistry Endothelial Cells - cytology Endothelial Cells - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Genotype & phenotype Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Intercellular Adhesion Molecule-1 - genetics Microscopy, Electron, Scanning Microspheres Miscellaneous Platelet Endothelial Cell Adhesion Molecule-1 - analysis poly(lactide-co-glycolide) Polyglactin 910 - chemistry Polystyrenes - chemistry Studies Tissue engineering Tissue Engineering - methods Tissue Scaffolds Umbilical Veins - cytology von Willebrand Factor - genetics |
| title | Human endothelial cell growth and phenotypic expression on three dimensional poly(lactide-co-glycolide) sintered microsphere scaffolds for bone tissue engineering |
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