Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites
The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite–host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachy...
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| Veröffentlicht in: | Molecular and biochemical parasitology 2007-02, Vol.151 (2), p.205-212 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Zhang, Houshuang Compaore, Muller K.A. Lee, Eung-goo Liao, Min Zhang, Guohong Sugimoto, Chihiro Fujisaki, Kozo Nishikawa, Yoshifumi Xuan, Xuenan |
| description | The cross-reactive antigens of
Neospora caninum and
Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite–host interaction. In this study, a gene encoding
N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a
N. caninum tachyzoite cDNA expression library with antisera from mice immunized with recombinant
T. gondii apical membrane antigen 1 (TgAMA1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of
N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by
N. caninum and
T. gondii. These results indicated that AMA1 is a cross-reactive antigen between
N. caninum and
T. gondii and a potential common vaccine candidate to control two parasites. |
| doi_str_mv | 10.1016/j.molbiopara.2006.11.005 |
| format | Article |
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Neospora caninum and
Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite–host interaction. In this study, a gene encoding
N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a
N. caninum tachyzoite cDNA expression library with antisera from mice immunized with recombinant
T. gondii apical membrane antigen 1 (TgAMA1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of
N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by
N. caninum and
T. gondii. These results indicated that AMA1 is a cross-reactive antigen between
N. caninum and
T. gondii and a potential common vaccine candidate to control two parasites.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/j.molbiopara.2006.11.005</identifier><identifier>PMID: 17156863</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Protozoan - immunology ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Apical membrane antigen 1 ; Cercopithecus aethiops ; Cloning, Molecular ; Cross Reactions ; Cross-reactive ; Host-Parasite Interactions ; Invasion ; Life Cycle Stages ; Molecular Sequence Data ; Neospora - growth & development ; Neospora - immunology ; Neospora - pathogenicity ; Neospora caninum ; Phylogeny ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Sequence Homology, Amino Acid ; Toxoplasma - growth & development ; Toxoplasma - immunology ; Toxoplasma - pathogenicity ; Toxoplasma gondii ; Vero Cells</subject><ispartof>Molecular and biochemical parasitology, 2007-02, Vol.151 (2), p.205-212</ispartof><rights>2006 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-ea16e740fe3cf337afa89ec3fe95adbcfc7007d74d932f7a03ceaff74f9b30c03</citedby><cites>FETCH-LOGICAL-c513t-ea16e740fe3cf337afa89ec3fe95adbcfc7007d74d932f7a03ceaff74f9b30c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166685106003276$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17156863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Houshuang</creatorcontrib><creatorcontrib>Compaore, Muller K.A.</creatorcontrib><creatorcontrib>Lee, Eung-goo</creatorcontrib><creatorcontrib>Liao, Min</creatorcontrib><creatorcontrib>Zhang, Guohong</creatorcontrib><creatorcontrib>Sugimoto, Chihiro</creatorcontrib><creatorcontrib>Fujisaki, Kozo</creatorcontrib><creatorcontrib>Nishikawa, Yoshifumi</creatorcontrib><creatorcontrib>Xuan, Xuenan</creatorcontrib><title>Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>The cross-reactive antigens of
Neospora caninum and
Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite–host interaction. In this study, a gene encoding
N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a
N. caninum tachyzoite cDNA expression library with antisera from mice immunized with recombinant
T. gondii apical membrane antigen 1 (TgAMA1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of
N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by
N. caninum and
T. gondii. These results indicated that AMA1 is a cross-reactive antigen between
N. caninum and
T. gondii and a potential common vaccine candidate to control two parasites.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Apical membrane antigen 1</subject><subject>Cercopithecus aethiops</subject><subject>Cloning, Molecular</subject><subject>Cross Reactions</subject><subject>Cross-reactive</subject><subject>Host-Parasite Interactions</subject><subject>Invasion</subject><subject>Life Cycle Stages</subject><subject>Molecular Sequence Data</subject><subject>Neospora - growth & development</subject><subject>Neospora - immunology</subject><subject>Neospora - pathogenicity</subject><subject>Neospora caninum</subject><subject>Phylogeny</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Sequence Homology, Amino Acid</subject><subject>Toxoplasma - growth & development</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - pathogenicity</subject><subject>Toxoplasma gondii</subject><subject>Vero Cells</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhFZBPnEiw442dHJcKClIpl3K2Js6461USB9u7Zd-KR6z3j7THnsYa_8bf-PsIoZyVnHH5ZVOOfuicnyFAWTEmS85LxupXZMEbVRXtsmpek0VGZSGbml-RdzFuWCaUlG_JFVe8lo0UC_J_NTsDAx1x7AJMSGFK7hEnyqmLFKgJPsYiIJjkdpfbDtMT5nqPPs4-ZA4mN23HDPT0wf_z8wBxBProp965z8d2Wp_mi3uz-rXix3Pn-z1109p1LkW69jFRg8OQWzuIzmehPe18WtPDT6NLGN-TNxaGiB_O9Zr8-f7t4eZHcff79ufN6q4wNRepQOAS1ZJZFMYKocBC06IRFtsa-s5YoxhTvVr2raisAiYMgrVqadtOMMPENfl0encO_u8WY9Kji4fdskl-G7VsllXdVC-DGWEVb3kGmxN49DSg1XNwI4S95kwfYtUbfYlVH2LVnOscWh79eNbYdiP2l8Fzjhn4egIwW7JzGHQ0DieDvQtoku69e1nlGV2GvVg</recordid><startdate>20070201</startdate><enddate>20070201</enddate><creator>Zhang, Houshuang</creator><creator>Compaore, Muller K.A.</creator><creator>Lee, Eung-goo</creator><creator>Liao, Min</creator><creator>Zhang, Guohong</creator><creator>Sugimoto, Chihiro</creator><creator>Fujisaki, Kozo</creator><creator>Nishikawa, Yoshifumi</creator><creator>Xuan, Xuenan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20070201</creationdate><title>Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites</title><author>Zhang, Houshuang ; Compaore, Muller K.A. ; Lee, Eung-goo ; Liao, Min ; Zhang, Guohong ; Sugimoto, Chihiro ; Fujisaki, Kozo ; Nishikawa, Yoshifumi ; Xuan, Xuenan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-ea16e740fe3cf337afa89ec3fe95adbcfc7007d74d932f7a03ceaff74f9b30c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Apical membrane antigen 1</topic><topic>Cercopithecus aethiops</topic><topic>Cloning, Molecular</topic><topic>Cross Reactions</topic><topic>Cross-reactive</topic><topic>Host-Parasite Interactions</topic><topic>Invasion</topic><topic>Life Cycle Stages</topic><topic>Molecular Sequence Data</topic><topic>Neospora - growth & development</topic><topic>Neospora - immunology</topic><topic>Neospora - pathogenicity</topic><topic>Neospora caninum</topic><topic>Phylogeny</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Sequence Homology, Amino Acid</topic><topic>Toxoplasma - growth & development</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma - pathogenicity</topic><topic>Toxoplasma gondii</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Houshuang</creatorcontrib><creatorcontrib>Compaore, Muller K.A.</creatorcontrib><creatorcontrib>Lee, Eung-goo</creatorcontrib><creatorcontrib>Liao, Min</creatorcontrib><creatorcontrib>Zhang, Guohong</creatorcontrib><creatorcontrib>Sugimoto, Chihiro</creatorcontrib><creatorcontrib>Fujisaki, Kozo</creatorcontrib><creatorcontrib>Nishikawa, Yoshifumi</creatorcontrib><creatorcontrib>Xuan, Xuenan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Houshuang</au><au>Compaore, Muller K.A.</au><au>Lee, Eung-goo</au><au>Liao, Min</au><au>Zhang, Guohong</au><au>Sugimoto, Chihiro</au><au>Fujisaki, Kozo</au><au>Nishikawa, Yoshifumi</au><au>Xuan, Xuenan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>2007-02-01</date><risdate>2007</risdate><volume>151</volume><issue>2</issue><spage>205</spage><epage>212</epage><pages>205-212</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><abstract>The cross-reactive antigens of
Neospora caninum and
Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite–host interaction. In this study, a gene encoding
N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a
N. caninum tachyzoite cDNA expression library with antisera from mice immunized with recombinant
T. gondii apical membrane antigen 1 (TgAMA1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by Western blotting. Immunofluorescence analysis showed that NcAMA1 was localized to the apical end of tachyzoites. Two-dimensional electrophoresis and Western blotting indicated that an approximately 57-kDa cleavage product was released into the excretory/secretory products of
N. caninum. Preincubation of free tachyzoites with anti-rNcAMA1 IgG antibodies inhibited the invasion into host cells by
N. caninum and
T. gondii. These results indicated that AMA1 is a cross-reactive antigen between
N. caninum and
T. gondii and a potential common vaccine candidate to control two parasites.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>17156863</pmid><doi>10.1016/j.molbiopara.2006.11.005</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular and biochemical parasitology, 2007-02, Vol.151 (2), p.205-212 |
| issn | 0166-6851 1872-9428 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Antibodies, Protozoan - immunology Antigens, Protozoan - genetics Antigens, Protozoan - immunology Apical membrane antigen 1 Cercopithecus aethiops Cloning, Molecular Cross Reactions Cross-reactive Host-Parasite Interactions Invasion Life Cycle Stages Molecular Sequence Data Neospora - growth & development Neospora - immunology Neospora - pathogenicity Neospora caninum Phylogeny Protozoan Proteins - genetics Protozoan Proteins - immunology Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Sequence Homology, Amino Acid Toxoplasma - growth & development Toxoplasma - immunology Toxoplasma - pathogenicity Toxoplasma gondii Vero Cells |
| title | Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites |
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