Parametric study of a 6-column countercurrent solvent gradient purification (MCSGP) unit
The novel "multicolumn countercurrent solvent gradient purification" (MCSGP) process has been modeled for the purification of a polypeptide mixture characterized by a strong non-linear competitive adsorption isotherm. As a model system, the purification of an industrial polypeptide mixture...
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| Veröffentlicht in: | Biotechnology and bioengineering 2007-12, Vol.98 (5), p.1029-1042 |
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| container_title | Biotechnology and bioengineering |
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| creator | Aumann, Lars Stroehlein, Guido Morbidelli, Massimo |
| description | The novel "multicolumn countercurrent solvent gradient purification" (MCSGP) process has been modeled for the purification of a polypeptide mixture characterized by a strong non-linear competitive adsorption isotherm. As a model system, the purification of an industrial polypeptide mixture containing 46% of the hormone calcitonin has been selected. The many impurities contained in the mixture have been lumped into three key impurities, which are selected as the ones eluting closer to the main component. The simulation model allows for a better understanding of the complex operating behavior of the multicolumn system, which has been experimentally investigated in a previous work. Through a systematic parametric analyses of the model behavior, the main operating parameters controlling the process performance in terms of purity and yield are investigated. The study of internal liquid and adsorbed phase concentration profiles along the unit for the different operating conditions allow elucidating the working principle of the new separation process. It is found that the MCSGP unit achieves much higher yields for a given product purity than the corresponding single-column batch units. Biotechnol. Bioeng. 2007;98: 1029-1042. © 2007 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.21529 |
| format | Article |
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As a model system, the purification of an industrial polypeptide mixture containing 46% of the hormone calcitonin has been selected. The many impurities contained in the mixture have been lumped into three key impurities, which are selected as the ones eluting closer to the main component. The simulation model allows for a better understanding of the complex operating behavior of the multicolumn system, which has been experimentally investigated in a previous work. Through a systematic parametric analyses of the model behavior, the main operating parameters controlling the process performance in terms of purity and yield are investigated. The study of internal liquid and adsorbed phase concentration profiles along the unit for the different operating conditions allow elucidating the working principle of the new separation process. It is found that the MCSGP unit achieves much higher yields for a given product purity than the corresponding single-column batch units. Biotechnol. Bioeng. 2007;98: 1029-1042. © 2007 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.21529</identifier><identifier>PMID: 17595046</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adsorption ; Algorithms ; Biological and medical sciences ; Biotechnology ; Biotechnology - methods ; Calcitonin - isolation & purification ; chromatographic modeling ; Computer Simulation ; continuous countercurrent solvent gradient ; Countercurrent Distribution - methods ; Fundamental and applied biological sciences. Psychology ; Hormones ; Kinetics ; MCSGP ; Methods. Procedures. 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Bioeng</addtitle><description>The novel "multicolumn countercurrent solvent gradient purification" (MCSGP) process has been modeled for the purification of a polypeptide mixture characterized by a strong non-linear competitive adsorption isotherm. As a model system, the purification of an industrial polypeptide mixture containing 46% of the hormone calcitonin has been selected. The many impurities contained in the mixture have been lumped into three key impurities, which are selected as the ones eluting closer to the main component. The simulation model allows for a better understanding of the complex operating behavior of the multicolumn system, which has been experimentally investigated in a previous work. Through a systematic parametric analyses of the model behavior, the main operating parameters controlling the process performance in terms of purity and yield are investigated. The study of internal liquid and adsorbed phase concentration profiles along the unit for the different operating conditions allow elucidating the working principle of the new separation process. It is found that the MCSGP unit achieves much higher yields for a given product purity than the corresponding single-column batch units. Biotechnol. Bioeng. 2007;98: 1029-1042. © 2007 Wiley Periodicals, Inc.</description><subject>Adsorption</subject><subject>Algorithms</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Calcitonin - isolation & purification</subject><subject>chromatographic modeling</subject><subject>Computer Simulation</subject><subject>continuous countercurrent solvent gradient</subject><subject>Countercurrent Distribution - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones</subject><subject>Kinetics</subject><subject>MCSGP</subject><subject>Methods. Procedures. Technologies</subject><subject>Models, Theoretical</subject><subject>multicomponent competitive bi-Langmuir isotherm</subject><subject>Others</subject><subject>peptide purification</subject><subject>Peptides</subject><subject>Peptides - isolation & purification</subject><subject>Rheology</subject><subject>Simulation</subject><subject>SMB</subject><subject>Solvents</subject><subject>Temperature</subject><subject>Various methods and equipments</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Utv1DAQAGALgehSOPAHIEIC0UNaexy_jjTAUqlAUVvBzfI6TuWSxIudAPvv8ZKFSkioJ9vSNw_PIPSY4EOCMRyt_HgIhIG6gxYEK1FiUPguWmCMeUmZgj30IKXr_BSS8_tojwimGK74An05M9H0bozeFmmcmk0R2sIUvLShm_qhsGEaRhftFKMbxiKF7vv2vIqm8dvLeoq-9daMPgzFy_f1-fLsoJgGPz5E91rTJfdod-6jy7dvLup35enH5Un96rS0rCKqXDlJYYUZo6xSynLREEWka8BQQsAKJ0klG8CmNZxxo4DJhsiKNkoS0RpC99GLOe86hm-TS6PufbKu68zgwpQ0lxUwKuFWSPPgIHdxKwQsBc3Dy_DZP_A6THHIv9VAqODA1TbbwYxsDClF1-p19L2JG02w3m5P5-3p39vL9sku4bTqXXMjd-vK4PkOmGRN10YzWJ9unAIqKKPZHc3uh-_c5v8V9fHJxZ_S5Rzh0-h-_o0w8avmggqmP39YaiZfH0NdV_pT9k9n35qgzVXMXVyeAyYUYwmY84r-ArX_xes</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Aumann, Lars</creator><creator>Stroehlein, Guido</creator><creator>Morbidelli, Massimo</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Parametric study of a 6-column countercurrent solvent gradient purification (MCSGP) unit</title><author>Aumann, Lars ; Stroehlein, Guido ; Morbidelli, Massimo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5419-be832b05535499c67d1918ed2a3112c7e8148d20afa656a9258d1843d9817fa13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adsorption</topic><topic>Algorithms</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Calcitonin - isolation & purification</topic><topic>chromatographic modeling</topic><topic>Computer Simulation</topic><topic>continuous countercurrent solvent gradient</topic><topic>Countercurrent Distribution - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones</topic><topic>Kinetics</topic><topic>MCSGP</topic><topic>Methods. Procedures. Technologies</topic><topic>Models, Theoretical</topic><topic>multicomponent competitive bi-Langmuir isotherm</topic><topic>Others</topic><topic>peptide purification</topic><topic>Peptides</topic><topic>Peptides - isolation & purification</topic><topic>Rheology</topic><topic>Simulation</topic><topic>SMB</topic><topic>Solvents</topic><topic>Temperature</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aumann, Lars</creatorcontrib><creatorcontrib>Stroehlein, Guido</creatorcontrib><creatorcontrib>Morbidelli, Massimo</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aumann, Lars</au><au>Stroehlein, Guido</au><au>Morbidelli, Massimo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Parametric study of a 6-column countercurrent solvent gradient purification (MCSGP) unit</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>98</volume><issue>5</issue><spage>1029</spage><epage>1042</epage><pages>1029-1042</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The novel "multicolumn countercurrent solvent gradient purification" (MCSGP) process has been modeled for the purification of a polypeptide mixture characterized by a strong non-linear competitive adsorption isotherm. As a model system, the purification of an industrial polypeptide mixture containing 46% of the hormone calcitonin has been selected. The many impurities contained in the mixture have been lumped into three key impurities, which are selected as the ones eluting closer to the main component. The simulation model allows for a better understanding of the complex operating behavior of the multicolumn system, which has been experimentally investigated in a previous work. Through a systematic parametric analyses of the model behavior, the main operating parameters controlling the process performance in terms of purity and yield are investigated. The study of internal liquid and adsorbed phase concentration profiles along the unit for the different operating conditions allow elucidating the working principle of the new separation process. It is found that the MCSGP unit achieves much higher yields for a given product purity than the corresponding single-column batch units. Biotechnol. Bioeng. 2007;98: 1029-1042. © 2007 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17595046</pmid><doi>10.1002/bit.21529</doi><tpages>14</tpages></addata></record> |
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| ispartof | Biotechnology and bioengineering, 2007-12, Vol.98 (5), p.1029-1042 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adsorption Algorithms Biological and medical sciences Biotechnology Biotechnology - methods Calcitonin - isolation & purification chromatographic modeling Computer Simulation continuous countercurrent solvent gradient Countercurrent Distribution - methods Fundamental and applied biological sciences. Psychology Hormones Kinetics MCSGP Methods. Procedures. Technologies Models, Theoretical multicomponent competitive bi-Langmuir isotherm Others peptide purification Peptides Peptides - isolation & purification Rheology Simulation SMB Solvents Temperature Various methods and equipments |
| title | Parametric study of a 6-column countercurrent solvent gradient purification (MCSGP) unit |
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