Isometric contraction of microvascular pericytes from mouse brain parenchyma

Pericytes were isolated and cultured from mouse cerebroparenchymal microvessels. A single pericyte clone was three-dimensionally cultured in a collagen gel by adding tensile stress, resulting in the reconstruction of narrow stringy fibers. When the contractility of these fibers was evaluated isometr...

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Veröffentlicht in:	Microvascular research 2007, Vol.73 (1), p.20-28
Hauptverfasser:	Oishi, Kazuhiko, Kamiyashiki, Tsutomu, Ito, Yuko
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholine - pharmacology Actins - analysis Actins - genetics Animals Antigens - analysis Atropine - pharmacology Brain - blood supply Brain parenchyma Calcium - deficiency Calcium - metabolism Capillaries - cytology Cells, Cultured Collagen gel fiber Contractility Dinoprost - pharmacology Gene Expression - drug effects Glutamyl Aminopeptidase - analysis Hyaluronan Receptors - analysis Intermediate Filament Proteins - analysis Intermediate Filament Proteins - genetics Isometric Contraction - drug effects Isometric Contraction - physiology Isometric force Male Mice Mice, Inbred C57BL Microcirculation - cytology Microvascular pericytes Nerve Tissue Proteins - analysis Nerve Tissue Proteins - genetics Nestin Norepinephrine - pharmacology Papaverine - pharmacology Pericytes - drug effects Pericytes - metabolism Pericytes - physiology Phenylephrine - pharmacology Proteoglycans - analysis Thy-1 Antigens - analysis Vimentin - genetics
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