Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis
From a Bacillus licheniformis wild type as well as a defined asporogenous derivative, stable UV hypersensitive mutants were generated by targeted deletion of the uvrBA operon, encoding highly conserved key components of the nucleotide excision repair. Comparative studies, which included the respecti...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2007, Vol.73 (6), p.1340-1347 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Waldeck, Jens Meyer-Rammes, Heike Nahrstedt, Hannes Eichstädt, Renée Wieland, Susanne Meinhardt, Friedhelm |
| description | From a Bacillus licheniformis wild type as well as a defined asporogenous derivative, stable UV hypersensitive mutants were generated by targeted deletion of the uvrBA operon, encoding highly conserved key components of the nucleotide excision repair. Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants. |
| doi_str_mv | 10.1007/s00253-006-0602-4 |
| format | Article |
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Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-006-0602-4</identifier><identifier>PMID: 17004053</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Bacillus - genetics ; Bacillus - growth & development ; Bacillus - radiation effects ; Bacillus licheniformis ; Bacteria ; batch fermentation ; biocontainment ; Biological and medical sciences ; Biotechnology ; Blotting, Southern ; Comparative analysis ; Comparative studies ; Containment ; Enzymes ; Extracellular enzymes ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Genes, Bacterial - genetics ; Hypersensitivity ; Industrial Bacillus ; Industrial Microbiology - methods ; Models, Genetic ; Mutation ; Operon - genetics ; Plant propagation ; Polymerase Chain Reaction ; SOS response ; SOS Response (Genetics) ; Studies ; Targeted gene deletion ; Ultraviolet Rays ; UV hypersensitivity</subject><ispartof>Applied microbiology and biotechnology, 2007, Vol.73 (6), p.1340-1347</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-1eca6b592bc09d7158f5d3efd898dc6e2da96caac403bf039761352d417708fb3</citedby><cites>FETCH-LOGICAL-c411t-1eca6b592bc09d7158f5d3efd898dc6e2da96caac403bf039761352d417708fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18445373$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17004053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Waldeck, Jens</creatorcontrib><creatorcontrib>Meyer-Rammes, Heike</creatorcontrib><creatorcontrib>Nahrstedt, Hannes</creatorcontrib><creatorcontrib>Eichstädt, Renée</creatorcontrib><creatorcontrib>Wieland, Susanne</creatorcontrib><creatorcontrib>Meinhardt, Friedhelm</creatorcontrib><title>Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>From a Bacillus licheniformis wild type as well as a defined asporogenous derivative, stable UV hypersensitive mutants were generated by targeted deletion of the uvrBA operon, encoding highly conserved key components of the nucleotide excision repair. Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants.</description><subject>Bacillus - genetics</subject><subject>Bacillus - growth & development</subject><subject>Bacillus - radiation effects</subject><subject>Bacillus licheniformis</subject><subject>Bacteria</subject><subject>batch fermentation</subject><subject>biocontainment</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>Comparative analysis</subject><subject>Comparative studies</subject><subject>Containment</subject><subject>Enzymes</subject><subject>Extracellular enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Genes, Bacterial - genetics</subject><subject>Hypersensitivity</subject><subject>Industrial Bacillus</subject><subject>Industrial Microbiology - methods</subject><subject>Models, Genetic</subject><subject>Mutation</subject><subject>Operon - genetics</subject><subject>Plant propagation</subject><subject>Polymerase Chain Reaction</subject><subject>SOS response</subject><subject>SOS Response (Genetics)</subject><subject>Studies</subject><subject>Targeted gene deletion</subject><subject>Ultraviolet Rays</subject><subject>UV hypersensitivity</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0UtrFTEUB_Agir1WP4AbDYLuRk8mj8ks2-ILCi5s1yGTx21KJrkmM0K_vRnvhYIbVyHJL-dw8kfoNYGPBGD4VAF6TjsA0YGAvmNP0I4w2rcdYU_RDsjAu4GP8gy9qPUegPRSiOfojAwADDjdofVGl71bnMXWRbeEnHD2eLlzeP1dLi9wPrjSznSyeAo55n0wOmKT06JDml1acEh_eUh2rUsJOsYHHOZDLotut5fahBjXimMwdy4Fn8sc6kv0zOtY3avTeo5uv3y-ufrWXf_4-v3q4rozjJClI85oMfGxnwyMdiBcem6p81aO0hrheqtHYbQ2DOjkgY6DIJT3lpFhAOkneo4-HOseSv61urqo1ty4GHVyea1KSNaD5Oy_kIztRznb4Lt_4H1eS2pDKEEkHymXQ0PkiEzJtRbn1aGEWZcHRUBtyaljcqolp7bk1Fb4zanwOs3OPr44RdXA-xPQtWXgi04m1EcnGeN02Nzbo_M6K70vzdz-7IHQ1pcKSQT9A_-Iqyo</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Waldeck, Jens</creator><creator>Meyer-Rammes, Heike</creator><creator>Nahrstedt, Hannes</creator><creator>Eichstädt, Renée</creator><creator>Wieland, Susanne</creator><creator>Meinhardt, Friedhelm</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis</title><author>Waldeck, Jens ; Meyer-Rammes, Heike ; Nahrstedt, Hannes ; Eichstädt, Renée ; Wieland, Susanne ; Meinhardt, Friedhelm</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-1eca6b592bc09d7158f5d3efd898dc6e2da96caac403bf039761352d417708fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bacillus - genetics</topic><topic>Bacillus - growth & development</topic><topic>Bacillus - radiation effects</topic><topic>Bacillus licheniformis</topic><topic>Bacteria</topic><topic>batch fermentation</topic><topic>biocontainment</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>Comparative analysis</topic><topic>Comparative studies</topic><topic>Containment</topic><topic>Enzymes</topic><topic>Extracellular enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Genes, Bacterial - genetics</topic><topic>Hypersensitivity</topic><topic>Industrial Bacillus</topic><topic>Industrial Microbiology - methods</topic><topic>Models, Genetic</topic><topic>Mutation</topic><topic>Operon - genetics</topic><topic>Plant propagation</topic><topic>Polymerase Chain Reaction</topic><topic>SOS response</topic><topic>SOS Response (Genetics)</topic><topic>Studies</topic><topic>Targeted gene deletion</topic><topic>Ultraviolet Rays</topic><topic>UV hypersensitivity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Waldeck, Jens</creatorcontrib><creatorcontrib>Meyer-Rammes, Heike</creatorcontrib><creatorcontrib>Nahrstedt, Hannes</creatorcontrib><creatorcontrib>Eichstädt, Renée</creatorcontrib><creatorcontrib>Wieland, Susanne</creatorcontrib><creatorcontrib>Meinhardt, Friedhelm</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - 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Comparative studies, which included the respective parental strains, revealed no negative side effects of the deletion, neither on enzyme secretion nor on vegetative propagation. Thus, the uvrBA locus proved to be a useful deletion target for achieving biological containment in this industrially exploited bacterium. In contrast to recA mutants, which also display UV hypersensitivity, further strain development via homologous recombination techniques will be still possible in such uvr mutants.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17004053</pmid><doi>10.1007/s00253-006-0602-4</doi><tpages>8</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 2007, Vol.73 (6), p.1340-1347 |
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| subjects | Bacillus - genetics Bacillus - growth & development Bacillus - radiation effects Bacillus licheniformis Bacteria batch fermentation biocontainment Biological and medical sciences Biotechnology Blotting, Southern Comparative analysis Comparative studies Containment Enzymes Extracellular enzymes Fundamental and applied biological sciences. Psychology Gene Deletion Genes, Bacterial - genetics Hypersensitivity Industrial Bacillus Industrial Microbiology - methods Models, Genetic Mutation Operon - genetics Plant propagation Polymerase Chain Reaction SOS response SOS Response (Genetics) Studies Targeted gene deletion Ultraviolet Rays UV hypersensitivity |
| title | Targeted deletion of the uvrBA operon and biological containment in the industrially important Bacillus licheniformis |
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