RESP18, a homolog of the luminal domain IA-2, is found in dense core vesicles in pancreatic islet cells and is induced by high glucose
The regulated endocrine-specific protein, RESP18, first found in the rat pituitary, was thought to be regulated by dopaminergic drugs. Bioinformatics studies showed that RESP18 shares sequence homology with the luminal region of IA-2, a dense core vesicle (DCV) transmembrane protein involved in insu...
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| Veröffentlicht in: | Journal of endocrinology 2007-11, Vol.195 (2), p.313-321 |
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| creator | Zhang, Guofeng Hirai, Hiroki Cai, Tao Miura, Junnosuke Yu, Ping Huang, Hanxia Schiller, Martin R Swaim, William D Leapman, Richard D Notkins, Abner L |
| description | The regulated endocrine-specific protein, RESP18, first found in the rat pituitary, was thought to be regulated by dopaminergic drugs. Bioinformatics studies showed that RESP18 shares sequence homology with the luminal region of IA-2, a dense core vesicle (DCV) transmembrane protein involved in insulin secretion. The present study was initiated to examine the genomic structure and subcellular localization of RESP18 and the effect of glucose on its expression. Human RESP18 was isolated from a pancreas cDNA library and its subcellular localization was determined by immunoelectron microscopy. MIN6 cells and mouse islets were used to study the effect of glucose on RESP18 expression. Bioinformatics analysis revealed that RESP18 and IA-2 are tandemly arranged within a 45 kb region on human chromosome 2 and share common intron–exon boundaries. By confocal microscopy, RESP18 was found in α, β and δ cells in the pancreatic islets. Electron microscopy revealed that RESP18 is present in the lumen of DCVs. The expression of RESP18 in β cells is markedly increased following exposure to high glucose and also elevated in the islets of diabetic, but not non-diabetic, NOD mice. We conclude that RESP18 is a luminal protein of DCVs and its expression is regulated by exposure to glucose. |
| doi_str_mv | 10.1677/JOE-07-0252 |
| format | Article |
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Bioinformatics studies showed that RESP18 shares sequence homology with the luminal region of IA-2, a dense core vesicle (DCV) transmembrane protein involved in insulin secretion. The present study was initiated to examine the genomic structure and subcellular localization of RESP18 and the effect of glucose on its expression. Human RESP18 was isolated from a pancreas cDNA library and its subcellular localization was determined by immunoelectron microscopy. MIN6 cells and mouse islets were used to study the effect of glucose on RESP18 expression. Bioinformatics analysis revealed that RESP18 and IA-2 are tandemly arranged within a 45 kb region on human chromosome 2 and share common intron–exon boundaries. By confocal microscopy, RESP18 was found in α, β and δ cells in the pancreatic islets. Electron microscopy revealed that RESP18 is present in the lumen of DCVs. The expression of RESP18 in β cells is markedly increased following exposure to high glucose and also elevated in the islets of diabetic, but not non-diabetic, NOD mice. We conclude that RESP18 is a luminal protein of DCVs and its expression is regulated by exposure to glucose.</description><identifier>ISSN: 0022-0795</identifier><identifier>EISSN: 1479-6805</identifier><identifier>DOI: 10.1677/JOE-07-0252</identifier><identifier>PMID: 17951542</identifier><identifier>CODEN: JOENAK</identifier><language>eng</language><publisher>Colchester: BioScientifica</publisher><subject>Animals ; Biological and medical sciences ; Cell Line, Tumor ; Chromosome Mapping ; Chromosomes, Human, Pair 2 ; Computational Biology ; Diabetes Mellitus, Type 1 - metabolism ; Dose-Response Relationship, Drug ; Endocrine pancreas ; Evolution, Molecular ; Fundamental and applied biological sciences. Psychology ; Genome, Human ; Glucose - administration & dosage ; Glucose - pharmacology ; Hormones. Régulation ; Humans ; Insulinoma - metabolism ; Insulinoma - pathology ; Islets of Langerhans - cytology ; Islets of Langerhans - metabolism ; Mice ; Mice, Inbred NOD ; Microscopy, Confocal ; Microscopy, Electron ; Microscopy, Immunoelectron ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Receptor-Like Protein Tyrosine Phosphatases, Class 8 - genetics ; Receptor-Like Protein Tyrosine Phosphatases, Class 8 - metabolism ; Regular papers ; Secretory Vesicles - metabolism ; Subcellular Fractions - metabolism ; Tissue Distribution ; Up-Regulation ; Vertebrates: endocrinology</subject><ispartof>Journal of endocrinology, 2007-11, Vol.195 (2), p.313-321</ispartof><rights>Society for Endocrinology</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b505t-fb3950acc88ddf9f7c3caad53231c9a87fa4e8fcdfae515fb2af3333c5f5bfb53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19222053$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17951542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Guofeng</creatorcontrib><creatorcontrib>Hirai, Hiroki</creatorcontrib><creatorcontrib>Cai, Tao</creatorcontrib><creatorcontrib>Miura, Junnosuke</creatorcontrib><creatorcontrib>Yu, Ping</creatorcontrib><creatorcontrib>Huang, Hanxia</creatorcontrib><creatorcontrib>Schiller, Martin R</creatorcontrib><creatorcontrib>Swaim, William D</creatorcontrib><creatorcontrib>Leapman, Richard D</creatorcontrib><creatorcontrib>Notkins, Abner L</creatorcontrib><title>RESP18, a homolog of the luminal domain IA-2, is found in dense core vesicles in pancreatic islet cells and is induced by high glucose</title><title>Journal of endocrinology</title><addtitle>J Endocrinol</addtitle><description>The regulated endocrine-specific protein, RESP18, first found in the rat pituitary, was thought to be regulated by dopaminergic drugs. Bioinformatics studies showed that RESP18 shares sequence homology with the luminal region of IA-2, a dense core vesicle (DCV) transmembrane protein involved in insulin secretion. The present study was initiated to examine the genomic structure and subcellular localization of RESP18 and the effect of glucose on its expression. Human RESP18 was isolated from a pancreas cDNA library and its subcellular localization was determined by immunoelectron microscopy. MIN6 cells and mouse islets were used to study the effect of glucose on RESP18 expression. Bioinformatics analysis revealed that RESP18 and IA-2 are tandemly arranged within a 45 kb region on human chromosome 2 and share common intron–exon boundaries. By confocal microscopy, RESP18 was found in α, β and δ cells in the pancreatic islets. Electron microscopy revealed that RESP18 is present in the lumen of DCVs. The expression of RESP18 in β cells is markedly increased following exposure to high glucose and also elevated in the islets of diabetic, but not non-diabetic, NOD mice. We conclude that RESP18 is a luminal protein of DCVs and its expression is regulated by exposure to glucose.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 2</subject><subject>Computational Biology</subject><subject>Diabetes Mellitus, Type 1 - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endocrine pancreas</subject><subject>Evolution, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Human</subject><subject>Glucose - administration & dosage</subject><subject>Glucose - pharmacology</subject><subject>Hormones. Régulation</subject><subject>Humans</subject><subject>Insulinoma - metabolism</subject><subject>Insulinoma - pathology</subject><subject>Islets of Langerhans - cytology</subject><subject>Islets of Langerhans - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Immunoelectron</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Receptor-Like Protein Tyrosine Phosphatases, Class 8 - genetics</subject><subject>Receptor-Like Protein Tyrosine Phosphatases, Class 8 - metabolism</subject><subject>Regular papers</subject><subject>Secretory Vesicles - metabolism</subject><subject>Subcellular Fractions - metabolism</subject><subject>Tissue Distribution</subject><subject>Up-Regulation</subject><subject>Vertebrates: endocrinology</subject><issn>0022-0795</issn><issn>1479-6805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9vFCEUx4mxsWv15N1wsZd2KjDLMnNsmq3WNGnjjzN5A49dDDOsMKPpP9C_Wya7psaDXEi-fN7j8YGQN5xd8JVS7z_drSumKiakeEYWfKnaatUw-ZwsGBOiHLXymLzM-TtjXHJVvyDHvGRcLsWCPH5ef7nnzTkFuo19DHFDo6PjFmmYej9AoDb24Ad6c1mJc-ozdXEaLC2JxSEjNTEh_YnZm4B5jncwmIQwelPogCM1GEKmMBfNgJ0MWto90K3fbOkmTCZmfEWOHISMrw_7Cfl2vf569bG6vftwc3V5W3WSybFyXd1KBsY0jbWudcrUBsDKWtTctNAoB0tsnLEOsDzQdQJcXZaRTnauk_UJOd333aX4Y8I86t7neUAYME5Zr5olb1s1g2d70KSYc0Knd8n3kB40Z3rWrot2zZSetRf67aHt1PVon9iD5wK8OwCQDQSXiiSfn7hWCMFkXTix52Y5v3xC3fmYjcdh9M4b-Pv2P59eivi-6B_2fxP_Bhipq5U</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Zhang, Guofeng</creator><creator>Hirai, Hiroki</creator><creator>Cai, Tao</creator><creator>Miura, Junnosuke</creator><creator>Yu, Ping</creator><creator>Huang, Hanxia</creator><creator>Schiller, Martin R</creator><creator>Swaim, William D</creator><creator>Leapman, Richard D</creator><creator>Notkins, Abner L</creator><general>BioScientifica</general><general>Portland Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>RESP18, a homolog of the luminal domain IA-2, is found in dense core vesicles in pancreatic islet cells and is induced by high glucose</title><author>Zhang, Guofeng ; Hirai, Hiroki ; Cai, Tao ; Miura, Junnosuke ; Yu, Ping ; Huang, Hanxia ; Schiller, Martin R ; Swaim, William D ; Leapman, Richard D ; Notkins, Abner L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b505t-fb3950acc88ddf9f7c3caad53231c9a87fa4e8fcdfae515fb2af3333c5f5bfb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 2</topic><topic>Computational Biology</topic><topic>Diabetes Mellitus, Type 1 - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endocrine pancreas</topic><topic>Evolution, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Human</topic><topic>Glucose - administration & dosage</topic><topic>Glucose - pharmacology</topic><topic>Hormones. Régulation</topic><topic>Humans</topic><topic>Insulinoma - metabolism</topic><topic>Insulinoma - pathology</topic><topic>Islets of Langerhans - cytology</topic><topic>Islets of Langerhans - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Immunoelectron</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Receptor-Like Protein Tyrosine Phosphatases, Class 8 - genetics</topic><topic>Receptor-Like Protein Tyrosine Phosphatases, Class 8 - metabolism</topic><topic>Regular papers</topic><topic>Secretory Vesicles - metabolism</topic><topic>Subcellular Fractions - metabolism</topic><topic>Tissue Distribution</topic><topic>Up-Regulation</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Guofeng</creatorcontrib><creatorcontrib>Hirai, Hiroki</creatorcontrib><creatorcontrib>Cai, Tao</creatorcontrib><creatorcontrib>Miura, Junnosuke</creatorcontrib><creatorcontrib>Yu, Ping</creatorcontrib><creatorcontrib>Huang, Hanxia</creatorcontrib><creatorcontrib>Schiller, Martin R</creatorcontrib><creatorcontrib>Swaim, William D</creatorcontrib><creatorcontrib>Leapman, Richard D</creatorcontrib><creatorcontrib>Notkins, Abner L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Guofeng</au><au>Hirai, Hiroki</au><au>Cai, Tao</au><au>Miura, Junnosuke</au><au>Yu, Ping</au><au>Huang, Hanxia</au><au>Schiller, Martin R</au><au>Swaim, William D</au><au>Leapman, Richard D</au><au>Notkins, Abner L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RESP18, a homolog of the luminal domain IA-2, is found in dense core vesicles in pancreatic islet cells and is induced by high glucose</atitle><jtitle>Journal of endocrinology</jtitle><addtitle>J Endocrinol</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>195</volume><issue>2</issue><spage>313</spage><epage>321</epage><pages>313-321</pages><issn>0022-0795</issn><eissn>1479-6805</eissn><coden>JOENAK</coden><abstract>The regulated endocrine-specific protein, RESP18, first found in the rat pituitary, was thought to be regulated by dopaminergic drugs. Bioinformatics studies showed that RESP18 shares sequence homology with the luminal region of IA-2, a dense core vesicle (DCV) transmembrane protein involved in insulin secretion. The present study was initiated to examine the genomic structure and subcellular localization of RESP18 and the effect of glucose on its expression. Human RESP18 was isolated from a pancreas cDNA library and its subcellular localization was determined by immunoelectron microscopy. MIN6 cells and mouse islets were used to study the effect of glucose on RESP18 expression. Bioinformatics analysis revealed that RESP18 and IA-2 are tandemly arranged within a 45 kb region on human chromosome 2 and share common intron–exon boundaries. By confocal microscopy, RESP18 was found in α, β and δ cells in the pancreatic islets. Electron microscopy revealed that RESP18 is present in the lumen of DCVs. The expression of RESP18 in β cells is markedly increased following exposure to high glucose and also elevated in the islets of diabetic, but not non-diabetic, NOD mice. We conclude that RESP18 is a luminal protein of DCVs and its expression is regulated by exposure to glucose.</abstract><cop>Colchester</cop><pub>BioScientifica</pub><pmid>17951542</pmid><doi>10.1677/JOE-07-0252</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Cell Line, Tumor Chromosome Mapping Chromosomes, Human, Pair 2 Computational Biology Diabetes Mellitus, Type 1 - metabolism Dose-Response Relationship, Drug Endocrine pancreas Evolution, Molecular Fundamental and applied biological sciences. Psychology Genome, Human Glucose - administration & dosage Glucose - pharmacology Hormones. Régulation Humans Insulinoma - metabolism Insulinoma - pathology Islets of Langerhans - cytology Islets of Langerhans - metabolism Mice Mice, Inbred NOD Microscopy, Confocal Microscopy, Electron Microscopy, Immunoelectron Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Receptor-Like Protein Tyrosine Phosphatases, Class 8 - genetics Receptor-Like Protein Tyrosine Phosphatases, Class 8 - metabolism Regular papers Secretory Vesicles - metabolism Subcellular Fractions - metabolism Tissue Distribution Up-Regulation Vertebrates: endocrinology |
| title | RESP18, a homolog of the luminal domain IA-2, is found in dense core vesicles in pancreatic islet cells and is induced by high glucose |
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