Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon
A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and 22 within 14 days post partum. Blood samples w...
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| Veröffentlicht in: | Tropical animal health and production 2007-06, Vol.39 (5), p.335-338 |
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| creator | Bayemi, P. H Nsongka, V. M Perera, B. M. A. O Cavestany, D Webb, E. C |
| description | A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and 22 within 14 days post partum. Blood samples were analysed and progesterone levels were deduced from a curve obtained from standard absorbance values (A ₄₅₀). Results show that 95.5% of postpartum cows had progesterone levels below 1 ng/ml, with the highest level being 0.75 ng/ml. The mean level was 0.5 ± 0.26 ng/ml. The cows in the 'pregnant group' had progesterone levels ranging from 3.5 to 17.5 ng/ml. This kit can be used for measuring progesterone levels in cattle. Levels of 1 ng/ml for two consecutive samples or one sample at or above 3 ng/ml are an indication of the presence of corpus luteum, while cows below 1 ng/ml will be in anoestrus. |
| doi_str_mv | 10.1007/s11250-007-9020-6 |
| format | Article |
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| ispartof | Tropical animal health and production, 2007-06, Vol.39 (5), p.335-338 |
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| subjects | Animals Cameroon Cattle Cattle - blood Corpus luteum Enzyme immunoassay Enzyme-linked immunosorbent assay Enzymes Female Immunoassay immunoassays Immunoenzyme Techniques - methods Immunoenzyme Techniques - veterinary Pregnancy Pregnancy Tests - methods Pregnancy Tests - veterinary Progesterone Progesterone - blood Reproducibility of Results Validation |
| title | Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon |
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