Trojan Horse Strategy in Agrobacterium Transformation: Abusing MAPK Defense Signaling
Nuclear import of transfer DNA (T-DNA) is a central event in Agrobacterium transformation of plant cells and is thought to occur by the hijacking of certain host cell proteins. The T-DNA-associated virulence protein VirE2 mediates this process by binding to the nuclear import machinery via the host...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2007-10, Vol.318 (5849), p.453-456 |
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| creator | Djamei, Armin Pitzschke, Andrea Nakagami, Hirofumi Rajh, Iva Hirt, Heribert |
| description | Nuclear import of transfer DNA (T-DNA) is a central event in Agrobacterium transformation of plant cells and is thought to occur by the hijacking of certain host cell proteins. The T-DNA-associated virulence protein VirE2 mediates this process by binding to the nuclear import machinery via the host cell factor VIP1, whose role in plants has been so far unknown. Here we show that VIP1 is a transcription factor that is a direct target of the Agrobacterium-induced mitogen-activated protein kinase (MAPK) MPK3. Upon phosphorylation by MPK3, VIP1 relocalizes from the cytoplasm to the nucleus and regulates the expression of the PR1 pathogenesis-related gene. MAPK-dependent phosphorylation of VIP1 is necessary for VIP1-mediated Agrobacterium T-DNA transfer, indicating that Agrobacterium abuses the MAPK-targeted VIP1 defense signaling pathway for nuclear delivery of the T-DNA complex as a Trojan horse. |
| doi_str_mv | 10.1126/science.1148110 |
| format | Article |
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The T-DNA-associated virulence protein VirE2 mediates this process by binding to the nuclear import machinery via the host cell factor VIP1, whose role in plants has been so far unknown. Here we show that VIP1 is a transcription factor that is a direct target of the Agrobacterium-induced mitogen-activated protein kinase (MAPK) MPK3. Upon phosphorylation by MPK3, VIP1 relocalizes from the cytoplasm to the nucleus and regulates the expression of the PR1 pathogenesis-related gene. MAPK-dependent phosphorylation of VIP1 is necessary for VIP1-mediated Agrobacterium T-DNA transfer, indicating that Agrobacterium abuses the MAPK-targeted VIP1 defense signaling pathway for nuclear delivery of the T-DNA complex as a Trojan horse.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1148110</identifier><identifier>PMID: 17947581</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Active Transport, Cell Nucleus ; Agrobacterium ; Agrobacterium tumefaciens - genetics ; Agrobacterium tumefaciens - pathogenicity ; Antibodies ; Arabidopsis - immunology ; Arabidopsis - metabolism ; Arabidopsis - microbiology ; Arabidopsis Proteins - metabolism ; Bacteria ; Bacterial plant pathogens ; Biological and medical sciences ; Cell Nucleus - metabolism ; Cytoplasm ; Cytoplasm - metabolism ; DNA, Bacterial - genetics ; DNA, Bacterial - metabolism ; DNA, Single-Stranded - genetics ; DNA, Single-Stranded - metabolism ; Enzyme Activation ; Flagellin - immunology ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Genomics ; Kinases ; MAP Kinase Signaling System ; Mitogen-Activated Protein Kinase Kinases - metabolism ; Pathology. Damages, economic importance ; Perceptual localization ; Phosphorylation ; Phytopathology. Animal pests. Plant and forest protection ; Plant cells ; Plant Leaves - metabolism ; Plant Leaves - microbiology ; Plant pathology ; Plants, Genetically Modified ; Proteins ; Protoplasts ; Recombinant Fusion Proteins - metabolism ; Signal transduction ; Transcription factors ; Transformation, Genetic ; Yeasts</subject><ispartof>Science (American Association for the Advancement of Science), 2007-10, Vol.318 (5849), p.453-456</ispartof><rights>Copyright 2007 American Association for the Advancement of Science</rights><rights>2008 INIST-CNRS</rights><rights>Copyright © 2007, American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-72b0b163c51396562947a8e7e0fe4b76307287d59c5e6e95825489a0cb6b402b3</citedby><cites>FETCH-LOGICAL-c500t-72b0b163c51396562947a8e7e0fe4b76307287d59c5e6e95825489a0cb6b402b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20051404$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20051404$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19193778$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17947581$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Djamei, Armin</creatorcontrib><creatorcontrib>Pitzschke, Andrea</creatorcontrib><creatorcontrib>Nakagami, Hirofumi</creatorcontrib><creatorcontrib>Rajh, Iva</creatorcontrib><creatorcontrib>Hirt, Heribert</creatorcontrib><title>Trojan Horse Strategy in Agrobacterium Transformation: Abusing MAPK Defense Signaling</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Nuclear import of transfer DNA (T-DNA) is a central event in Agrobacterium transformation of plant cells and is thought to occur by the hijacking of certain host cell proteins. The T-DNA-associated virulence protein VirE2 mediates this process by binding to the nuclear import machinery via the host cell factor VIP1, whose role in plants has been so far unknown. Here we show that VIP1 is a transcription factor that is a direct target of the Agrobacterium-induced mitogen-activated protein kinase (MAPK) MPK3. Upon phosphorylation by MPK3, VIP1 relocalizes from the cytoplasm to the nucleus and regulates the expression of the PR1 pathogenesis-related gene. MAPK-dependent phosphorylation of VIP1 is necessary for VIP1-mediated Agrobacterium T-DNA transfer, indicating that Agrobacterium abuses the MAPK-targeted VIP1 defense signaling pathway for nuclear delivery of the T-DNA complex as a Trojan horse.</description><subject>Active Transport, Cell Nucleus</subject><subject>Agrobacterium</subject><subject>Agrobacterium tumefaciens - genetics</subject><subject>Agrobacterium tumefaciens - pathogenicity</subject><subject>Antibodies</subject><subject>Arabidopsis - immunology</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Bacteria</subject><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoplasm</subject><subject>Cytoplasm - metabolism</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Single-Stranded - genetics</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>Enzyme Activation</subject><subject>Flagellin - immunology</subject><subject>Fundamental and applied biological sciences. 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| subjects | Active Transport, Cell Nucleus Agrobacterium Agrobacterium tumefaciens - genetics Agrobacterium tumefaciens - pathogenicity Antibodies Arabidopsis - immunology Arabidopsis - metabolism Arabidopsis - microbiology Arabidopsis Proteins - metabolism Bacteria Bacterial plant pathogens Biological and medical sciences Cell Nucleus - metabolism Cytoplasm Cytoplasm - metabolism DNA, Bacterial - genetics DNA, Bacterial - metabolism DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism Enzyme Activation Flagellin - immunology Fundamental and applied biological sciences. Psychology Gene expression regulation Genomics Kinases MAP Kinase Signaling System Mitogen-Activated Protein Kinase Kinases - metabolism Pathology. Damages, economic importance Perceptual localization Phosphorylation Phytopathology. Animal pests. Plant and forest protection Plant cells Plant Leaves - metabolism Plant Leaves - microbiology Plant pathology Plants, Genetically Modified Proteins Protoplasts Recombinant Fusion Proteins - metabolism Signal transduction Transcription factors Transformation, Genetic Yeasts |
| title | Trojan Horse Strategy in Agrobacterium Transformation: Abusing MAPK Defense Signaling |
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