Mechanisms involved in the vasodilator effect induced by diosgenin in rat superior mesenteric artery
The aim of this study was to investigate the vasorelaxant effect induced by diosgenin in superior mesenteric rings. In rings pre-contracted with phenylephrine (10μM), diosgenin caused concentration-dependent relaxations [EC 50 = (3.3 ± 1.2) × 10 − 4M, E max = 94.2 ± 2.6 %]. Vascular relaxation induc...
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| Veröffentlicht in: | European journal of pharmacology 2007-11, Vol.574 (2), p.172-178 |
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| creator | Dias, Katy Lísias Gondim Correia, Nadja de Azevedo Pereira, Krísthea Karyne Gonçalves Barbosa-Filho, José Maria Cavalcante, Karla Veruska Marques Araújo, Islania Giselia Albuquerque Silva, Darizy Flávia Guedes, Diego Nunes Neto, Mario dos Anjos Bendhack, Lusiane Maria Medeiros, Isac Almeida |
| description | The aim of this study was to investigate the vasorelaxant effect induced by diosgenin in superior mesenteric rings. In rings pre-contracted with phenylephrine (10μM), diosgenin caused concentration-dependent relaxations [EC
50 = (3.3 ± 1.2) × 10
− 4M,
E
max = 94.2 ± 2.6 %]. Vascular relaxation induced by diosgenin was significantly inhibited after removal of the endothelium (
E
max = 46 ± 8.8%,
p < 0.001) or after pre-treatment of the rings with
N-nitro-
l-arginine methyl esther (
l-NAME) 100 or 300μM (
E
max = 35.3 ± 4%; 28.1 ± 3.3%, respectively,
p < 0.001), atropine 1μM (
E
max = 24.6 ± 3.4%,
p < 0.001), hydroxocobalamin 30μM (
E
max = 54.0 ± 9.6%,
p < 0.001), 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ) 10μM (
E
max = 46.0 ± 8.0%,
p < 0.001) or indomethacin 1μM (
E
max = 22.6 ± 11.8%,
p < 0.001). Vasorelaxation evoked by diosgenin was significantly inhibited after pre-treatment of preparations with both selective and non-selective inhibitors of large conductance Ca
2+-activated K
+ (BK
Ca) channels, iberiotoxin 100nM or tetraethylammonium (TEA) 1mM, respectively (
E
max = 62.5 ± 9.1%; 65.7 ± 1.1%,
p < 0.001). Conversely, in endothelium-denuded vessels, none of BK
Ca channel blockers modified the relaxant effect induced by diosgenin. In mesenteric endothelial cells loaded with FURA-2 diosgenin was able to increase intracellular calcium concentrations, which were significantly decreased by atropine 1μM. In addition, in isolated mesenteric rings, diosgenin induced marked increase in nitric oxide (NO) levels, which was completely abolished after functional endothelium removal. The results obtained here demonstrated that diosgenin-induced relaxation appears to involve endothelial muscarinic receptor activation with increase in intracellular calcium concentrations and consequent release of endothelium-derived relaxing factors (EDRFs), mainly NO and cyclooxygenase derivatives, which activate BK
Ca channels. Nevertheless, further studies are necessary to clearly elucidate residual endothelium-independent relaxation induced by diosgenin. |
| doi_str_mv | 10.1016/j.ejphar.2007.07.017 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68412475</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014299907007960</els_id><sourcerecordid>68412475</sourcerecordid><originalsourceid>FETCH-LOGICAL-c305t-be11cf4877a7211360581f7e257d2780e596dadcd84fda173ee7a56e5c5202373</originalsourceid><addsrcrecordid>eNp9kE1r4zAQhsXS0qbd_oNl8aW9OdXIlmVdFkrpF7T00p6FIo03Cv7IauxA_v3KJNBb4YUZ0KOX4WHsF_AlcKhuN0vcbNc2LgXnajkH1A-2gFrpnCsQJ2zBOZS50FqfswuiDedcaiHP2DmoqtZSlAvm39CtbR-ooyz0u6HdoU9LNq4x21kafGjtOMQMmwbdmF785BKx2mc-DPQX-8SmRDtmNG0xhsR2SNiPaXeZjWnuf7LTxraEV8d5yT4fHz7un_PX96eX-7vX3BVcjvkKAVxT1kpZJQCKissaGoVCKi9UzVHqylvvfF023oIqEJWVFUonBReFKi7ZzaF3G4d_E9JoukAO29b2OExkqroEUSqZwPIAujgQRWzMNobOxr0Bbma7ZmMOds1s18yBuf_3sX9adei_Ph11JuD6CFhytm2i7V2gL05DXVV6Lvpz4DDZ2AWMhlzAPpkNMWk2fgjfX_IfypWbdA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68412475</pqid></control><display><type>article</type><title>Mechanisms involved in the vasodilator effect induced by diosgenin in rat superior mesenteric artery</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Dias, Katy Lísias Gondim ; Correia, Nadja de Azevedo ; Pereira, Krísthea Karyne Gonçalves ; Barbosa-Filho, José Maria ; Cavalcante, Karla Veruska Marques ; Araújo, Islania Giselia Albuquerque ; Silva, Darizy Flávia ; Guedes, Diego Nunes ; Neto, Mario dos Anjos ; Bendhack, Lusiane Maria ; Medeiros, Isac Almeida</creator><creatorcontrib>Dias, Katy Lísias Gondim ; Correia, Nadja de Azevedo ; Pereira, Krísthea Karyne Gonçalves ; Barbosa-Filho, José Maria ; Cavalcante, Karla Veruska Marques ; Araújo, Islania Giselia Albuquerque ; Silva, Darizy Flávia ; Guedes, Diego Nunes ; Neto, Mario dos Anjos ; Bendhack, Lusiane Maria ; Medeiros, Isac Almeida</creatorcontrib><description><![CDATA[The aim of this study was to investigate the vasorelaxant effect induced by diosgenin in superior mesenteric rings. In rings pre-contracted with phenylephrine (10μM), diosgenin caused concentration-dependent relaxations [EC
50 = (3.3 ± 1.2) × 10
− 4M,
E
max = 94.2 ± 2.6 %]. Vascular relaxation induced by diosgenin was significantly inhibited after removal of the endothelium (
E
max = 46 ± 8.8%,
p < 0.001) or after pre-treatment of the rings with
N-nitro-
l-arginine methyl esther (
l-NAME) 100 or 300μM (
E
max = 35.3 ± 4%; 28.1 ± 3.3%, respectively,
p < 0.001), atropine 1μM (
E
max = 24.6 ± 3.4%,
p < 0.001), hydroxocobalamin 30μM (
E
max = 54.0 ± 9.6%,
p < 0.001), 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ) 10μM (
E
max = 46.0 ± 8.0%,
p < 0.001) or indomethacin 1μM (
E
max = 22.6 ± 11.8%,
p < 0.001). Vasorelaxation evoked by diosgenin was significantly inhibited after pre-treatment of preparations with both selective and non-selective inhibitors of large conductance Ca
2+-activated K
+ (BK
Ca) channels, iberiotoxin 100nM or tetraethylammonium (TEA) 1mM, respectively (
E
max = 62.5 ± 9.1%; 65.7 ± 1.1%,
p < 0.001). Conversely, in endothelium-denuded vessels, none of BK
Ca channel blockers modified the relaxant effect induced by diosgenin. In mesenteric endothelial cells loaded with FURA-2 diosgenin was able to increase intracellular calcium concentrations, which were significantly decreased by atropine 1μM. In addition, in isolated mesenteric rings, diosgenin induced marked increase in nitric oxide (NO) levels, which was completely abolished after functional endothelium removal. The results obtained here demonstrated that diosgenin-induced relaxation appears to involve endothelial muscarinic receptor activation with increase in intracellular calcium concentrations and consequent release of endothelium-derived relaxing factors (EDRFs), mainly NO and cyclooxygenase derivatives, which activate BK
Ca channels. Nevertheless, further studies are necessary to clearly elucidate residual endothelium-independent relaxation induced by diosgenin.]]></description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2007.07.017</identifier><identifier>PMID: 17689524</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Calcium - metabolism ; Diosgenin ; Diosgenin - pharmacology ; Dose-Response Relationship, Drug ; Endothelium-Dependent Relaxing Factors - physiology ; Intracellular Ca 2 ; Large-Conductance Calcium-Activated Potassium Channels - physiology ; Male ; Medical sciences ; Mesenteric artery ; Mesenteric Artery, Superior - drug effects ; Mesenteric Artery, Superior - physiology ; Nitric oxide ; Nitric Oxide - physiology ; Pharmacology. Drug treatments ; Phenylephrine - pharmacology ; Potassium Channel Blockers - pharmacology ; Prostaglandin-Endoperoxide Synthases - physiology ; Rats ; Rats, Wistar ; Vasodilation ; Vasodilation - drug effects ; Vasodilator Agents - pharmacology</subject><ispartof>European journal of pharmacology, 2007-11, Vol.574 (2), p.172-178</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-be11cf4877a7211360581f7e257d2780e596dadcd84fda173ee7a56e5c5202373</citedby><cites>FETCH-LOGICAL-c305t-be11cf4877a7211360581f7e257d2780e596dadcd84fda173ee7a56e5c5202373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejphar.2007.07.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19186697$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17689524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dias, Katy Lísias Gondim</creatorcontrib><creatorcontrib>Correia, Nadja de Azevedo</creatorcontrib><creatorcontrib>Pereira, Krísthea Karyne Gonçalves</creatorcontrib><creatorcontrib>Barbosa-Filho, José Maria</creatorcontrib><creatorcontrib>Cavalcante, Karla Veruska Marques</creatorcontrib><creatorcontrib>Araújo, Islania Giselia Albuquerque</creatorcontrib><creatorcontrib>Silva, Darizy Flávia</creatorcontrib><creatorcontrib>Guedes, Diego Nunes</creatorcontrib><creatorcontrib>Neto, Mario dos Anjos</creatorcontrib><creatorcontrib>Bendhack, Lusiane Maria</creatorcontrib><creatorcontrib>Medeiros, Isac Almeida</creatorcontrib><title>Mechanisms involved in the vasodilator effect induced by diosgenin in rat superior mesenteric artery</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description><![CDATA[The aim of this study was to investigate the vasorelaxant effect induced by diosgenin in superior mesenteric rings. In rings pre-contracted with phenylephrine (10μM), diosgenin caused concentration-dependent relaxations [EC
50 = (3.3 ± 1.2) × 10
− 4M,
E
max = 94.2 ± 2.6 %]. Vascular relaxation induced by diosgenin was significantly inhibited after removal of the endothelium (
E
max = 46 ± 8.8%,
p < 0.001) or after pre-treatment of the rings with
N-nitro-
l-arginine methyl esther (
l-NAME) 100 or 300μM (
E
max = 35.3 ± 4%; 28.1 ± 3.3%, respectively,
p < 0.001), atropine 1μM (
E
max = 24.6 ± 3.4%,
p < 0.001), hydroxocobalamin 30μM (
E
max = 54.0 ± 9.6%,
p < 0.001), 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ) 10μM (
E
max = 46.0 ± 8.0%,
p < 0.001) or indomethacin 1μM (
E
max = 22.6 ± 11.8%,
p < 0.001). Vasorelaxation evoked by diosgenin was significantly inhibited after pre-treatment of preparations with both selective and non-selective inhibitors of large conductance Ca
2+-activated K
+ (BK
Ca) channels, iberiotoxin 100nM or tetraethylammonium (TEA) 1mM, respectively (
E
max = 62.5 ± 9.1%; 65.7 ± 1.1%,
p < 0.001). Conversely, in endothelium-denuded vessels, none of BK
Ca channel blockers modified the relaxant effect induced by diosgenin. In mesenteric endothelial cells loaded with FURA-2 diosgenin was able to increase intracellular calcium concentrations, which were significantly decreased by atropine 1μM. In addition, in isolated mesenteric rings, diosgenin induced marked increase in nitric oxide (NO) levels, which was completely abolished after functional endothelium removal. The results obtained here demonstrated that diosgenin-induced relaxation appears to involve endothelial muscarinic receptor activation with increase in intracellular calcium concentrations and consequent release of endothelium-derived relaxing factors (EDRFs), mainly NO and cyclooxygenase derivatives, which activate BK
Ca channels. Nevertheless, further studies are necessary to clearly elucidate residual endothelium-independent relaxation induced by diosgenin.]]></description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Diosgenin</subject><subject>Diosgenin - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelium-Dependent Relaxing Factors - physiology</subject><subject>Intracellular Ca 2</subject><subject>Large-Conductance Calcium-Activated Potassium Channels - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mesenteric artery</subject><subject>Mesenteric Artery, Superior - drug effects</subject><subject>Mesenteric Artery, Superior - physiology</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - physiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenylephrine - pharmacology</subject><subject>Potassium Channel Blockers - pharmacology</subject><subject>Prostaglandin-Endoperoxide Synthases - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vasodilation</subject><subject>Vasodilation - drug effects</subject><subject>Vasodilator Agents - pharmacology</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r4zAQhsXS0qbd_oNl8aW9OdXIlmVdFkrpF7T00p6FIo03Cv7IauxA_v3KJNBb4YUZ0KOX4WHsF_AlcKhuN0vcbNc2LgXnajkH1A-2gFrpnCsQJ2zBOZS50FqfswuiDedcaiHP2DmoqtZSlAvm39CtbR-ooyz0u6HdoU9LNq4x21kafGjtOMQMmwbdmF785BKx2mc-DPQX-8SmRDtmNG0xhsR2SNiPaXeZjWnuf7LTxraEV8d5yT4fHz7un_PX96eX-7vX3BVcjvkKAVxT1kpZJQCKissaGoVCKi9UzVHqylvvfF023oIqEJWVFUonBReFKi7ZzaF3G4d_E9JoukAO29b2OExkqroEUSqZwPIAujgQRWzMNobOxr0Bbma7ZmMOds1s18yBuf_3sX9adei_Ph11JuD6CFhytm2i7V2gL05DXVV6Lvpz4DDZ2AWMhlzAPpkNMWk2fgjfX_IfypWbdA</recordid><startdate>20071128</startdate><enddate>20071128</enddate><creator>Dias, Katy Lísias Gondim</creator><creator>Correia, Nadja de Azevedo</creator><creator>Pereira, Krísthea Karyne Gonçalves</creator><creator>Barbosa-Filho, José Maria</creator><creator>Cavalcante, Karla Veruska Marques</creator><creator>Araújo, Islania Giselia Albuquerque</creator><creator>Silva, Darizy Flávia</creator><creator>Guedes, Diego Nunes</creator><creator>Neto, Mario dos Anjos</creator><creator>Bendhack, Lusiane Maria</creator><creator>Medeiros, Isac Almeida</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071128</creationdate><title>Mechanisms involved in the vasodilator effect induced by diosgenin in rat superior mesenteric artery</title><author>Dias, Katy Lísias Gondim ; Correia, Nadja de Azevedo ; Pereira, Krísthea Karyne Gonçalves ; Barbosa-Filho, José Maria ; Cavalcante, Karla Veruska Marques ; Araújo, Islania Giselia Albuquerque ; Silva, Darizy Flávia ; Guedes, Diego Nunes ; Neto, Mario dos Anjos ; Bendhack, Lusiane Maria ; Medeiros, Isac Almeida</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-be11cf4877a7211360581f7e257d2780e596dadcd84fda173ee7a56e5c5202373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Diosgenin</topic><topic>Diosgenin - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelium-Dependent Relaxing Factors - physiology</topic><topic>Intracellular Ca 2</topic><topic>Large-Conductance Calcium-Activated Potassium Channels - physiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mesenteric artery</topic><topic>Mesenteric Artery, Superior - drug effects</topic><topic>Mesenteric Artery, Superior - physiology</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - physiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenylephrine - pharmacology</topic><topic>Potassium Channel Blockers - pharmacology</topic><topic>Prostaglandin-Endoperoxide Synthases - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vasodilation</topic><topic>Vasodilation - drug effects</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dias, Katy Lísias Gondim</creatorcontrib><creatorcontrib>Correia, Nadja de Azevedo</creatorcontrib><creatorcontrib>Pereira, Krísthea Karyne Gonçalves</creatorcontrib><creatorcontrib>Barbosa-Filho, José Maria</creatorcontrib><creatorcontrib>Cavalcante, Karla Veruska Marques</creatorcontrib><creatorcontrib>Araújo, Islania Giselia Albuquerque</creatorcontrib><creatorcontrib>Silva, Darizy Flávia</creatorcontrib><creatorcontrib>Guedes, Diego Nunes</creatorcontrib><creatorcontrib>Neto, Mario dos Anjos</creatorcontrib><creatorcontrib>Bendhack, Lusiane Maria</creatorcontrib><creatorcontrib>Medeiros, Isac Almeida</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dias, Katy Lísias Gondim</au><au>Correia, Nadja de Azevedo</au><au>Pereira, Krísthea Karyne Gonçalves</au><au>Barbosa-Filho, José Maria</au><au>Cavalcante, Karla Veruska Marques</au><au>Araújo, Islania Giselia Albuquerque</au><au>Silva, Darizy Flávia</au><au>Guedes, Diego Nunes</au><au>Neto, Mario dos Anjos</au><au>Bendhack, Lusiane Maria</au><au>Medeiros, Isac Almeida</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanisms involved in the vasodilator effect induced by diosgenin in rat superior mesenteric artery</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2007-11-28</date><risdate>2007</risdate><volume>574</volume><issue>2</issue><spage>172</spage><epage>178</epage><pages>172-178</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract><![CDATA[The aim of this study was to investigate the vasorelaxant effect induced by diosgenin in superior mesenteric rings. In rings pre-contracted with phenylephrine (10μM), diosgenin caused concentration-dependent relaxations [EC
50 = (3.3 ± 1.2) × 10
− 4M,
E
max = 94.2 ± 2.6 %]. Vascular relaxation induced by diosgenin was significantly inhibited after removal of the endothelium (
E
max = 46 ± 8.8%,
p < 0.001) or after pre-treatment of the rings with
N-nitro-
l-arginine methyl esther (
l-NAME) 100 or 300μM (
E
max = 35.3 ± 4%; 28.1 ± 3.3%, respectively,
p < 0.001), atropine 1μM (
E
max = 24.6 ± 3.4%,
p < 0.001), hydroxocobalamin 30μM (
E
max = 54.0 ± 9.6%,
p < 0.001), 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ) 10μM (
E
max = 46.0 ± 8.0%,
p < 0.001) or indomethacin 1μM (
E
max = 22.6 ± 11.8%,
p < 0.001). Vasorelaxation evoked by diosgenin was significantly inhibited after pre-treatment of preparations with both selective and non-selective inhibitors of large conductance Ca
2+-activated K
+ (BK
Ca) channels, iberiotoxin 100nM or tetraethylammonium (TEA) 1mM, respectively (
E
max = 62.5 ± 9.1%; 65.7 ± 1.1%,
p < 0.001). Conversely, in endothelium-denuded vessels, none of BK
Ca channel blockers modified the relaxant effect induced by diosgenin. In mesenteric endothelial cells loaded with FURA-2 diosgenin was able to increase intracellular calcium concentrations, which were significantly decreased by atropine 1μM. In addition, in isolated mesenteric rings, diosgenin induced marked increase in nitric oxide (NO) levels, which was completely abolished after functional endothelium removal. The results obtained here demonstrated that diosgenin-induced relaxation appears to involve endothelial muscarinic receptor activation with increase in intracellular calcium concentrations and consequent release of endothelium-derived relaxing factors (EDRFs), mainly NO and cyclooxygenase derivatives, which activate BK
Ca channels. Nevertheless, further studies are necessary to clearly elucidate residual endothelium-independent relaxation induced by diosgenin.]]></abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17689524</pmid><doi>10.1016/j.ejphar.2007.07.017</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-2999 |
| ispartof | European journal of pharmacology, 2007-11, Vol.574 (2), p.172-178 |
| issn | 0014-2999 1879-0712 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68412475 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Calcium - metabolism Diosgenin Diosgenin - pharmacology Dose-Response Relationship, Drug Endothelium-Dependent Relaxing Factors - physiology Intracellular Ca 2 Large-Conductance Calcium-Activated Potassium Channels - physiology Male Medical sciences Mesenteric artery Mesenteric Artery, Superior - drug effects Mesenteric Artery, Superior - physiology Nitric oxide Nitric Oxide - physiology Pharmacology. Drug treatments Phenylephrine - pharmacology Potassium Channel Blockers - pharmacology Prostaglandin-Endoperoxide Synthases - physiology Rats Rats, Wistar Vasodilation Vasodilation - drug effects Vasodilator Agents - pharmacology |
| title | Mechanisms involved in the vasodilator effect induced by diosgenin in rat superior mesenteric artery |
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