Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice
Transgenic rice plants expressing 7Crp peptide were generated by Agrobacterium-mediated transformation. The 7Crp peptide is the hybrid peptide of seven major human T-cell epitopes derived from Japanese cedar pollen allergens Cry j 1 and Cry j 2. When the 7Crp gene was expressed under the control of...
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| Veröffentlicht in: | Plant biotechnology journal 2007-01, Vol.5 (1), p.84-92 |
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| creator | Takaiwa, Fumio Takagi, Hidenori Hirose, Sakiko Wakasa, Yuhya |
| description | Transgenic rice plants expressing 7Crp peptide were generated by Agrobacterium-mediated transformation. The 7Crp peptide is the hybrid peptide of seven major human T-cell epitopes derived from Japanese cedar pollen allergens Cry j 1 and Cry j 2. When the 7Crp gene was expressed under the control of the rice AGPase large subunit or maize ubiquitin-1 promoters, it could only be detected in the endosperm of rice seed, although high levels of RNA transcript were observed in the leaf, stem, and seed embryo. It was demonstrated by confocal and electron microscopy analysis that the 7Crp peptide was mainly localized in the endoplasmic reticulum-derived protein bodies, designated protein body I (PB-I). Our results indicate that rice endosperm tissue has advantage over other tissues as a production platform for foreign recombinant proteins. |
| doi_str_mv | 10.1111/j.1467-7652.2006.00220.x |
| format | Article |
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The 7Crp peptide is the hybrid peptide of seven major human T-cell epitopes derived from Japanese cedar pollen allergens Cry j 1 and Cry j 2. When the 7Crp gene was expressed under the control of the rice AGPase large subunit or maize ubiquitin-1 promoters, it could only be detected in the endosperm of rice seed, although high levels of RNA transcript were observed in the leaf, stem, and seed embryo. It was demonstrated by confocal and electron microscopy analysis that the 7Crp peptide was mainly localized in the endoplasmic reticulum-derived protein bodies, designated protein body I (PB-I). Our results indicate that rice endosperm tissue has advantage over other tissues as a production platform for foreign recombinant proteins.</description><subject>7Crp</subject><subject>AGPase large subunit promoter</subject><subject>Allergens</subject><subject>Bacterial Proteins - genetics</subject><subject>endosperm tissue</subject><subject>Oryza - genetics</subject><subject>Plant Proteins - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Pollen</subject><subject>Promoter Regions, Genetic</subject><subject>protein body</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Rhizobium - genetics</subject><subject>transgenic rice</subject><subject>Trees</subject><subject>Ubiquitin - genetics</subject><subject>ubiquitin promoter</subject><subject>Zea mays - genetics</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAURiNERUvhFcArdgn-iZ2xxAaG0latChK0XVoe-2bkIYmntiOmb1-HjKbbemFfyee79j1FgQiuSF6fNxWpRVM2gtOKYiwqjCnF1e5VcXK4eH2o6_q4eBvjJkNEcPGmOCYNxQ3l8qQwZ4P1cQuhR8nFOAJyEa29t2gbvB1Ncn5A206n1mckb0iH5FpnnO5QAOP7lRv0kCY8gRsicgNKQQ9xDYMzKDgD74qjVncR3u_P0-L2x9mf5UV5_fP8cvn1ujS1rHFJ6pUk3HIuMdWsBau10ZILveDWYGYklnkCziypqSArgoHnAQUFybQFBuy0-DT3zX95GCEm1btooOv0AH6MSixqgnnDM7iYQRN8jAFatQ2u1-FREawmwWqjJndq8qgmweq_YLXL0Q_7N8ZVD_Y5uDeagS8z8M918PjixurXt8tc5Hg5x11MsDvEdfirRMMaru5vzpW4v7m6-363VCLzH2e-1V7pdXBR3f6mmDBMCGVCUvYEYpaiyw</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Takaiwa, Fumio</creator><creator>Takagi, Hidenori</creator><creator>Hirose, Sakiko</creator><creator>Wakasa, Yuhya</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200701</creationdate><title>Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice</title><author>Takaiwa, Fumio ; Takagi, Hidenori ; Hirose, Sakiko ; Wakasa, Yuhya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4940-14b915d55902a3fedaaca956a85dc03c90900253d14261b10e514662e93ade3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>7Crp</topic><topic>AGPase large subunit promoter</topic><topic>Allergens</topic><topic>Bacterial Proteins - genetics</topic><topic>endosperm tissue</topic><topic>Oryza - genetics</topic><topic>Plant Proteins - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Pollen</topic><topic>Promoter Regions, Genetic</topic><topic>protein body</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Rhizobium - genetics</topic><topic>transgenic rice</topic><topic>Trees</topic><topic>Ubiquitin - genetics</topic><topic>ubiquitin promoter</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takaiwa, Fumio</creatorcontrib><creatorcontrib>Takagi, Hidenori</creatorcontrib><creatorcontrib>Hirose, Sakiko</creatorcontrib><creatorcontrib>Wakasa, Yuhya</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Takaiwa, Fumio</au><au>Takagi, Hidenori</au><au>Hirose, Sakiko</au><au>Wakasa, Yuhya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2007-01</date><risdate>2007</risdate><volume>5</volume><issue>1</issue><spage>84</spage><epage>92</epage><pages>84-92</pages><issn>1467-7644</issn><eissn>1467-7652</eissn><abstract>Transgenic rice plants expressing 7Crp peptide were generated by Agrobacterium-mediated transformation. The 7Crp peptide is the hybrid peptide of seven major human T-cell epitopes derived from Japanese cedar pollen allergens Cry j 1 and Cry j 2. When the 7Crp gene was expressed under the control of the rice AGPase large subunit or maize ubiquitin-1 promoters, it could only be detected in the endosperm of rice seed, although high levels of RNA transcript were observed in the leaf, stem, and seed embryo. It was demonstrated by confocal and electron microscopy analysis that the 7Crp peptide was mainly localized in the endoplasmic reticulum-derived protein bodies, designated protein body I (PB-I). Our results indicate that rice endosperm tissue has advantage over other tissues as a production platform for foreign recombinant proteins.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17207259</pmid><doi>10.1111/j.1467-7652.2006.00220.x</doi><tpages>9</tpages></addata></record> |
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| subjects | 7Crp AGPase large subunit promoter Allergens Bacterial Proteins - genetics endosperm tissue Oryza - genetics Plant Proteins - genetics Plants, Genetically Modified Pollen Promoter Regions, Genetic protein body Recombinant Proteins - biosynthesis Rhizobium - genetics transgenic rice Trees Ubiquitin - genetics ubiquitin promoter Zea mays - genetics |
| title | Endosperm tissue is good production platform for artificial recombinant proteins in transgenic rice |
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