Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant
Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants - MSV subtypes A1-A6 - causes severe e...
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| Veröffentlicht in: | Journal of general virology 2007-11, Vol.88 (11), p.3154-3165 |
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| creator | Owor, B.E Martin, D.P Shepherd, D.N Edema, R Monjane, A.L Rybicki, E.P Thomson, J.A Varsani, A |
| description | Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants - MSV subtypes A1-A6 - causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR-restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for >60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species. |
| doi_str_mv | 10.1099/vir.0.83144-0 |
| format | Article |
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Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants - MSV subtypes A1-A6 - causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR-restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for >60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.83144-0</identifier><identifier>PMID: 17947543</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Base Sequence ; Biological and medical sciences ; corn ; DNA Fingerprinting ; epidemiological studies ; Evolution, Molecular ; Fundamental and applied biological sciences. Psychology ; Geminivirus ; genetic techniques and protocols ; Genetics ; Genome, Viral - genetics ; genomics ; Genotype ; Maize streak virus ; Maize streak virus - classification ; Maize streak virus - genetics ; Maize streak virus - isolation & purification ; microbial genetics ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; nucleotide sequences ; Phylogeny ; Plant Diseases - virology ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; recombinant virus variant ; Recombination, Genetic ; restriction fragment length polymorphism ; Sequence Analysis, DNA ; Sequence Homology ; Uganda ; Virology ; virus isolates ; Zea mays ; Zea mays - virology</subject><ispartof>Journal of general virology, 2007-11, Vol.88 (11), p.3154-3165</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-5251a97d362aaf19d8735f8f66e73278f41eeb0d9a03a0fac323bfd2b12471183</citedby><cites>FETCH-LOGICAL-c449t-5251a97d362aaf19d8735f8f66e73278f41eeb0d9a03a0fac323bfd2b12471183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19209371$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17947543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Owor, B.E</creatorcontrib><creatorcontrib>Martin, D.P</creatorcontrib><creatorcontrib>Shepherd, D.N</creatorcontrib><creatorcontrib>Edema, R</creatorcontrib><creatorcontrib>Monjane, A.L</creatorcontrib><creatorcontrib>Rybicki, E.P</creatorcontrib><creatorcontrib>Thomson, J.A</creatorcontrib><creatorcontrib>Varsani, A</creatorcontrib><title>Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants - MSV subtypes A1-A6 - causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR-restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for >60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>corn</subject><subject>DNA Fingerprinting</subject><subject>epidemiological studies</subject><subject>Evolution, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geminivirus</subject><subject>genetic techniques and protocols</subject><subject>Genetics</subject><subject>Genome, Viral - genetics</subject><subject>genomics</subject><subject>Genotype</subject><subject>Maize streak virus</subject><subject>Maize streak virus - classification</subject><subject>Maize streak virus - genetics</subject><subject>Maize streak virus - isolation & purification</subject><subject>microbial genetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Plant Diseases - virology</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>recombinant virus variant</subject><subject>Recombination, Genetic</subject><subject>restriction fragment length polymorphism</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Uganda</subject><subject>Virology</subject><subject>virus isolates</subject><subject>Zea mays</subject><subject>Zea mays - virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0bFv1DAUBnALgej1YGQFLyAx5PCzndgeqwoKUiUGuNl6SeyrIYkPO7mq_PX4eid1ZPLg3_ue5Y-QN8A2wIz5dAhpwzZagJQVe0ZWIJu64uXmOVkxxnkFAtQFucz5F2MF1eoluQBlpKqlWJH5xk1uDh3FCYeHHDKNno4Y_jqa5-TwNy0LlkxDjgPOLlOf4ki3O5x6pMkdHA6Z3ofe5X3RPe1DGQvtMoc4HaOOqItjGyacZnrAFMr5irzwZc69Pp9rsv3y-ef11-r2-82366vbqpPSzFXNa0CjetFwRA-m10rUXvumcUpwpb0E51rWG2QCmcdOcNH6nrfApQLQYk0-nHL3Kf5ZXJ7tGHLnhgEnF5dsGy2Z0UL9F4LRBjivC6xOsEsx5-S83acwYnqwwOyxD1u-yzL72Idlxb89By_t6PonfS6ggPdngLnDwSecupCfnClVCgXFfTy5u7C7uw_J2Z2bxlCe0YZ4XKq1BbACSuiavDtZj9HiLpW87Q_OQDCmG64aLf4BrAispA</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Owor, B.E</creator><creator>Martin, D.P</creator><creator>Shepherd, D.N</creator><creator>Edema, R</creator><creator>Monjane, A.L</creator><creator>Rybicki, E.P</creator><creator>Thomson, J.A</creator><creator>Varsani, A</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071101</creationdate><title>Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant</title><author>Owor, B.E ; Martin, D.P ; Shepherd, D.N ; Edema, R ; Monjane, A.L ; Rybicki, E.P ; Thomson, J.A ; Varsani, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-5251a97d362aaf19d8735f8f66e73278f41eeb0d9a03a0fac323bfd2b12471183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>corn</topic><topic>DNA Fingerprinting</topic><topic>epidemiological studies</topic><topic>Evolution, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geminivirus</topic><topic>genetic techniques and protocols</topic><topic>Genetics</topic><topic>Genome, Viral - genetics</topic><topic>genomics</topic><topic>Genotype</topic><topic>Maize streak virus</topic><topic>Maize streak virus - classification</topic><topic>Maize streak virus - genetics</topic><topic>Maize streak virus - isolation & purification</topic><topic>microbial genetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Plant Diseases - virology</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>recombinant virus variant</topic><topic>Recombination, Genetic</topic><topic>restriction fragment length polymorphism</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Uganda</topic><topic>Virology</topic><topic>virus isolates</topic><topic>Zea mays</topic><topic>Zea mays - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Owor, B.E</creatorcontrib><creatorcontrib>Martin, D.P</creatorcontrib><creatorcontrib>Shepherd, D.N</creatorcontrib><creatorcontrib>Edema, R</creatorcontrib><creatorcontrib>Monjane, A.L</creatorcontrib><creatorcontrib>Rybicki, E.P</creatorcontrib><creatorcontrib>Thomson, J.A</creatorcontrib><creatorcontrib>Varsani, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Owor, B.E</au><au>Martin, D.P</au><au>Shepherd, D.N</au><au>Edema, R</au><au>Monjane, A.L</au><au>Rybicki, E.P</au><au>Thomson, J.A</au><au>Varsani, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2007-11-01</date><risdate>2007</risdate><volume>88</volume><issue>11</issue><spage>3154</spage><epage>3165</epage><pages>3154-3165</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Maize streak virus (MSV) contributes significantly to the problem of extremely low African maize yields. Whilst a diverse range of MSV and MSV-like viruses are endemic in sub-Saharan Africa and neighbouring islands, only a single group of maize-adapted variants - MSV subtypes A1-A6 - causes severe enough disease in maize to influence yields substantially. In order to assist in designing effective strategies to control MSV in maize, a large survey covering 155 locations was conducted to assess the diversity, distribution and genetic characteristics of the Ugandan MSV-A population. PCR-restriction fragment-length polymorphism analyses of 391 virus isolates identified 49 genetic variants. Sixty-two full-genome sequences were determined, 52 of which were detectably recombinant. All but two recombinants contained predominantly MSV-A1-like sequences. Of the ten distinct recombination events observed, seven involved inter-MSV-A subtype recombination and three involved intra-MSV-A1 recombination. One of the intra-MSV-A1 recombinants, designated MSV-A1UgIII, accounted for >60 % of all MSV infections sampled throughout Uganda. Although recombination may be an important factor in the emergence of novel geminivirus variants, it is demonstrated that its characteristics in MSV are quite different from those observed in related African cassava-infecting geminivirus species.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17947543</pmid><doi>10.1099/vir.0.83144-0</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Base Sequence Biological and medical sciences corn DNA Fingerprinting epidemiological studies Evolution, Molecular Fundamental and applied biological sciences. Psychology Geminivirus genetic techniques and protocols Genetics Genome, Viral - genetics genomics Genotype Maize streak virus Maize streak virus - classification Maize streak virus - genetics Maize streak virus - isolation & purification microbial genetics Microbiology Miscellaneous Molecular Sequence Data nucleotide sequences Phylogeny Plant Diseases - virology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length recombinant virus variant Recombination, Genetic restriction fragment length polymorphism Sequence Analysis, DNA Sequence Homology Uganda Virology virus isolates Zea mays Zea mays - virology |
| title | Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant |
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