Evaluation of quantity and staining pattern of human papillomavirus (HPV)‐infected epithelial cells in thin‐layer cervical specimens using optimized HPV‐CARD assay
BACKGROUND. Testing for human papillomavirus (HPV) is used in the triage of women with a cervical cytology of atypical squamous cells of undetermined significance (ASCUS). A fluorescent in situ hybridization assay was developed for the detection of HPV using the catalyzed receptor deposition techniq...
Gespeichert in:
| Veröffentlicht in: | Cancer 2007-10, Vol.111 (5), p.330-338 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 338 |
|---|---|
| container_issue | 5 |
| container_start_page | 330 |
| container_title | Cancer |
| container_volume | 111 |
| creator | Algeciras‐Schimnich, Alicia Policht, Frank Sitailo, Svetlana Song, Minghao Morrison, Larry Sokolova, Irina |
| description | BACKGROUND.
Testing for human papillomavirus (HPV) is used in the triage of women with a cervical cytology of atypical squamous cells of undetermined significance (ASCUS). A fluorescent in situ hybridization assay was developed for the detection of HPV using the catalyzed receptor deposition technique (HPV‐CARD). In this study, the utility of this assay was tested for the detection of HPV in liquid‐based cervical cytology specimens.
METHODS.
A total of 195 liquid‐based cytology specimens were analyzed using the HPV‐CARD assay. The results from the assay were compared with HPV polymerase chain reaction (PCR) and typing results. The number of HPV‐infected cells and the staining pattern was correlated with the cytology classification.
RESULTS.
A 91% concordance between HPV‐CARD and PCR was observed for the detection of high‐risk HPV viruses. A 78% concordance was observed for specimens that were negative for HPV. In ASCUS, low‐grade squamous intraepithelial lesion (LSIL), and high‐grade squamous intraepithelial lesion (HSIL) categories, the average number of HPV‐positive cells per slide was 19 cells, 127 cells, and 450 cells, respectively. The number of cells with a punctate staining, suggestive of HPV integration, was 21% in ASCUS, 34% in LSIL, and 46% in HSIL specimens.
CONCLUSIONS.
The results of the current study indicate positive correlations between the severity of the disease and the increased overall quantity of HPV‐positive epithelial cells in cervical cytology specimens and accumulation of cells with punctate staining suggestive of integrated HPV. In summary, the developed HPV‐CARD assay was found to provide novel information regarding the proportion and staining pattern of HPV‐infected epithelial cells in different cytologic categories of cervical specimens. Cancer (Cancer Cytopathol) 2007. © 2007 American Cancer Society.
A fluorescence in situ hybridization assay was developed for the detection of the human papillomavirus (HPV) using the catalyzed receptor deposition technique. The utility of this assay was tested in detecting high‐risk HPV in liquid‐based cytology specimens. The assay appears to be useful in determining the proportion and staining pattern of HPV‐infected epithelial cells in cytologic specimens. |
| doi_str_mv | 10.1002/cncr.22946 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_68407986</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68407986</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3636-ea48ad3151369b6031ef30af468d0df915fb4d81e89e844d8d9ca8510331558a3</originalsourceid><addsrcrecordid>eNp9kcFu1DAQhi0EokvhwgMgnxAgpdhx4jjHKhSKVLWoAsQtmnUmrJHjpLazKJx4hL5GX4snwdtdiRsnj2c-fyPrJ-Q5Zyecsfytdtqf5HldyAdkxVldZYwX-UOyYoyprCzEtyPyJIQf6VrlpXhMjnhV5YWs6hW5O9uCnSGa0dGxpzczuGjiQsF1NEQwzrjvdIIY0d8Dm3kAlxqTsXYcYGv8HOir809fX__5fWtcjzpiR3EycYPWgKUarQ3UOBo3xiXGwoI-df3W6DQOE2ozoAt0DrtV4xTNYH4lR3ImvDm9fkchBFiekkc92IDPDucx-fL-7HNznl1cffjYnF5kWkghM4RCQSd4yYWs15IJjr1g0BdSdazra17266JTHFWNqkhVV2tQJWcivSkViGPycu-d_HgzY4jtYMLuF-BwnEMrVcGqWskEvtmD2o8heOzbyZsB_NJy1u6CaXfBtPfBJPjFwTqvB-z-oYckEsD3wE9jcfmPqm0um-u99C8N5Z7i</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68407986</pqid></control><display><type>article</type><title>Evaluation of quantity and staining pattern of human papillomavirus (HPV)‐infected epithelial cells in thin‐layer cervical specimens using optimized HPV‐CARD assay</title><source>MEDLINE</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Algeciras‐Schimnich, Alicia ; Policht, Frank ; Sitailo, Svetlana ; Song, Minghao ; Morrison, Larry ; Sokolova, Irina</creator><creatorcontrib>Algeciras‐Schimnich, Alicia ; Policht, Frank ; Sitailo, Svetlana ; Song, Minghao ; Morrison, Larry ; Sokolova, Irina</creatorcontrib><description>BACKGROUND.
Testing for human papillomavirus (HPV) is used in the triage of women with a cervical cytology of atypical squamous cells of undetermined significance (ASCUS). A fluorescent in situ hybridization assay was developed for the detection of HPV using the catalyzed receptor deposition technique (HPV‐CARD). In this study, the utility of this assay was tested for the detection of HPV in liquid‐based cervical cytology specimens.
METHODS.
A total of 195 liquid‐based cytology specimens were analyzed using the HPV‐CARD assay. The results from the assay were compared with HPV polymerase chain reaction (PCR) and typing results. The number of HPV‐infected cells and the staining pattern was correlated with the cytology classification.
RESULTS.
A 91% concordance between HPV‐CARD and PCR was observed for the detection of high‐risk HPV viruses. A 78% concordance was observed for specimens that were negative for HPV. In ASCUS, low‐grade squamous intraepithelial lesion (LSIL), and high‐grade squamous intraepithelial lesion (HSIL) categories, the average number of HPV‐positive cells per slide was 19 cells, 127 cells, and 450 cells, respectively. The number of cells with a punctate staining, suggestive of HPV integration, was 21% in ASCUS, 34% in LSIL, and 46% in HSIL specimens.
CONCLUSIONS.
The results of the current study indicate positive correlations between the severity of the disease and the increased overall quantity of HPV‐positive epithelial cells in cervical cytology specimens and accumulation of cells with punctate staining suggestive of integrated HPV. In summary, the developed HPV‐CARD assay was found to provide novel information regarding the proportion and staining pattern of HPV‐infected epithelial cells in different cytologic categories of cervical specimens. Cancer (Cancer Cytopathol) 2007. © 2007 American Cancer Society.
A fluorescence in situ hybridization assay was developed for the detection of the human papillomavirus (HPV) using the catalyzed receptor deposition technique. The utility of this assay was tested in detecting high‐risk HPV in liquid‐based cytology specimens. The assay appears to be useful in determining the proportion and staining pattern of HPV‐infected epithelial cells in cytologic specimens.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/cncr.22946</identifier><identifier>PMID: 17724679</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Carcinoma, Squamous Cell - virology ; catalyzed receptor deposition assay ; cervical cancer ; Cervical Intraepithelial Neoplasia - virology ; Colposcopy ; DNA Probes, HPV ; DNA, Viral ; Epithelial Cells - virology ; Female ; fluorescence in situ hybridization ; Follow-Up Studies ; human papillomavirus ; Humans ; In Situ Hybridization, Fluorescence ; Papillomaviridae - isolation & purification ; Papillomavirus Infections - virology ; Sensitivity and Specificity ; Uterine Cervical Neoplasms - virology ; Vaginal Smears</subject><ispartof>Cancer, 2007-10, Vol.111 (5), p.330-338</ispartof><rights>Copyright © 2007 American Cancer Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3636-ea48ad3151369b6031ef30af468d0df915fb4d81e89e844d8d9ca8510331558a3</citedby><cites>FETCH-LOGICAL-c3636-ea48ad3151369b6031ef30af468d0df915fb4d81e89e844d8d9ca8510331558a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcncr.22946$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcncr.22946$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17724679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Algeciras‐Schimnich, Alicia</creatorcontrib><creatorcontrib>Policht, Frank</creatorcontrib><creatorcontrib>Sitailo, Svetlana</creatorcontrib><creatorcontrib>Song, Minghao</creatorcontrib><creatorcontrib>Morrison, Larry</creatorcontrib><creatorcontrib>Sokolova, Irina</creatorcontrib><title>Evaluation of quantity and staining pattern of human papillomavirus (HPV)‐infected epithelial cells in thin‐layer cervical specimens using optimized HPV‐CARD assay</title><title>Cancer</title><addtitle>Cancer</addtitle><description>BACKGROUND.
Testing for human papillomavirus (HPV) is used in the triage of women with a cervical cytology of atypical squamous cells of undetermined significance (ASCUS). A fluorescent in situ hybridization assay was developed for the detection of HPV using the catalyzed receptor deposition technique (HPV‐CARD). In this study, the utility of this assay was tested for the detection of HPV in liquid‐based cervical cytology specimens.
METHODS.
A total of 195 liquid‐based cytology specimens were analyzed using the HPV‐CARD assay. The results from the assay were compared with HPV polymerase chain reaction (PCR) and typing results. The number of HPV‐infected cells and the staining pattern was correlated with the cytology classification.
RESULTS.
A 91% concordance between HPV‐CARD and PCR was observed for the detection of high‐risk HPV viruses. A 78% concordance was observed for specimens that were negative for HPV. In ASCUS, low‐grade squamous intraepithelial lesion (LSIL), and high‐grade squamous intraepithelial lesion (HSIL) categories, the average number of HPV‐positive cells per slide was 19 cells, 127 cells, and 450 cells, respectively. The number of cells with a punctate staining, suggestive of HPV integration, was 21% in ASCUS, 34% in LSIL, and 46% in HSIL specimens.
CONCLUSIONS.
The results of the current study indicate positive correlations between the severity of the disease and the increased overall quantity of HPV‐positive epithelial cells in cervical cytology specimens and accumulation of cells with punctate staining suggestive of integrated HPV. In summary, the developed HPV‐CARD assay was found to provide novel information regarding the proportion and staining pattern of HPV‐infected epithelial cells in different cytologic categories of cervical specimens. Cancer (Cancer Cytopathol) 2007. © 2007 American Cancer Society.
A fluorescence in situ hybridization assay was developed for the detection of the human papillomavirus (HPV) using the catalyzed receptor deposition technique. The utility of this assay was tested in detecting high‐risk HPV in liquid‐based cytology specimens. The assay appears to be useful in determining the proportion and staining pattern of HPV‐infected epithelial cells in cytologic specimens.</description><subject>Carcinoma, Squamous Cell - virology</subject><subject>catalyzed receptor deposition assay</subject><subject>cervical cancer</subject><subject>Cervical Intraepithelial Neoplasia - virology</subject><subject>Colposcopy</subject><subject>DNA Probes, HPV</subject><subject>DNA, Viral</subject><subject>Epithelial Cells - virology</subject><subject>Female</subject><subject>fluorescence in situ hybridization</subject><subject>Follow-Up Studies</subject><subject>human papillomavirus</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Papillomaviridae - isolation & purification</subject><subject>Papillomavirus Infections - virology</subject><subject>Sensitivity and Specificity</subject><subject>Uterine Cervical Neoplasms - virology</subject><subject>Vaginal Smears</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhwgMgnxAgpdhx4jjHKhSKVLWoAsQtmnUmrJHjpLazKJx4hL5GX4snwdtdiRsnj2c-fyPrJ-Q5Zyecsfytdtqf5HldyAdkxVldZYwX-UOyYoyprCzEtyPyJIQf6VrlpXhMjnhV5YWs6hW5O9uCnSGa0dGxpzczuGjiQsF1NEQwzrjvdIIY0d8Dm3kAlxqTsXYcYGv8HOir809fX__5fWtcjzpiR3EycYPWgKUarQ3UOBo3xiXGwoI-df3W6DQOE2ozoAt0DrtV4xTNYH4lR3ImvDm9fkchBFiekkc92IDPDucx-fL-7HNznl1cffjYnF5kWkghM4RCQSd4yYWs15IJjr1g0BdSdazra17266JTHFWNqkhVV2tQJWcivSkViGPycu-d_HgzY4jtYMLuF-BwnEMrVcGqWskEvtmD2o8heOzbyZsB_NJy1u6CaXfBtPfBJPjFwTqvB-z-oYckEsD3wE9jcfmPqm0um-u99C8N5Z7i</recordid><startdate>20071025</startdate><enddate>20071025</enddate><creator>Algeciras‐Schimnich, Alicia</creator><creator>Policht, Frank</creator><creator>Sitailo, Svetlana</creator><creator>Song, Minghao</creator><creator>Morrison, Larry</creator><creator>Sokolova, Irina</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071025</creationdate><title>Evaluation of quantity and staining pattern of human papillomavirus (HPV)‐infected epithelial cells in thin‐layer cervical specimens using optimized HPV‐CARD assay</title><author>Algeciras‐Schimnich, Alicia ; Policht, Frank ; Sitailo, Svetlana ; Song, Minghao ; Morrison, Larry ; Sokolova, Irina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3636-ea48ad3151369b6031ef30af468d0df915fb4d81e89e844d8d9ca8510331558a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Carcinoma, Squamous Cell - virology</topic><topic>catalyzed receptor deposition assay</topic><topic>cervical cancer</topic><topic>Cervical Intraepithelial Neoplasia - virology</topic><topic>Colposcopy</topic><topic>DNA Probes, HPV</topic><topic>DNA, Viral</topic><topic>Epithelial Cells - virology</topic><topic>Female</topic><topic>fluorescence in situ hybridization</topic><topic>Follow-Up Studies</topic><topic>human papillomavirus</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Papillomaviridae - isolation & purification</topic><topic>Papillomavirus Infections - virology</topic><topic>Sensitivity and Specificity</topic><topic>Uterine Cervical Neoplasms - virology</topic><topic>Vaginal Smears</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Algeciras‐Schimnich, Alicia</creatorcontrib><creatorcontrib>Policht, Frank</creatorcontrib><creatorcontrib>Sitailo, Svetlana</creatorcontrib><creatorcontrib>Song, Minghao</creatorcontrib><creatorcontrib>Morrison, Larry</creatorcontrib><creatorcontrib>Sokolova, Irina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Algeciras‐Schimnich, Alicia</au><au>Policht, Frank</au><au>Sitailo, Svetlana</au><au>Song, Minghao</au><au>Morrison, Larry</au><au>Sokolova, Irina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of quantity and staining pattern of human papillomavirus (HPV)‐infected epithelial cells in thin‐layer cervical specimens using optimized HPV‐CARD assay</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>2007-10-25</date><risdate>2007</risdate><volume>111</volume><issue>5</issue><spage>330</spage><epage>338</epage><pages>330-338</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><abstract>BACKGROUND.
Testing for human papillomavirus (HPV) is used in the triage of women with a cervical cytology of atypical squamous cells of undetermined significance (ASCUS). A fluorescent in situ hybridization assay was developed for the detection of HPV using the catalyzed receptor deposition technique (HPV‐CARD). In this study, the utility of this assay was tested for the detection of HPV in liquid‐based cervical cytology specimens.
METHODS.
A total of 195 liquid‐based cytology specimens were analyzed using the HPV‐CARD assay. The results from the assay were compared with HPV polymerase chain reaction (PCR) and typing results. The number of HPV‐infected cells and the staining pattern was correlated with the cytology classification.
RESULTS.
A 91% concordance between HPV‐CARD and PCR was observed for the detection of high‐risk HPV viruses. A 78% concordance was observed for specimens that were negative for HPV. In ASCUS, low‐grade squamous intraepithelial lesion (LSIL), and high‐grade squamous intraepithelial lesion (HSIL) categories, the average number of HPV‐positive cells per slide was 19 cells, 127 cells, and 450 cells, respectively. The number of cells with a punctate staining, suggestive of HPV integration, was 21% in ASCUS, 34% in LSIL, and 46% in HSIL specimens.
CONCLUSIONS.
The results of the current study indicate positive correlations between the severity of the disease and the increased overall quantity of HPV‐positive epithelial cells in cervical cytology specimens and accumulation of cells with punctate staining suggestive of integrated HPV. In summary, the developed HPV‐CARD assay was found to provide novel information regarding the proportion and staining pattern of HPV‐infected epithelial cells in different cytologic categories of cervical specimens. Cancer (Cancer Cytopathol) 2007. © 2007 American Cancer Society.
A fluorescence in situ hybridization assay was developed for the detection of the human papillomavirus (HPV) using the catalyzed receptor deposition technique. The utility of this assay was tested in detecting high‐risk HPV in liquid‐based cytology specimens. The assay appears to be useful in determining the proportion and staining pattern of HPV‐infected epithelial cells in cytologic specimens.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17724679</pmid><doi>10.1002/cncr.22946</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-543X |
| ispartof | Cancer, 2007-10, Vol.111 (5), p.330-338 |
| issn | 0008-543X 1097-0142 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68407986 |
| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Carcinoma, Squamous Cell - virology catalyzed receptor deposition assay cervical cancer Cervical Intraepithelial Neoplasia - virology Colposcopy DNA Probes, HPV DNA, Viral Epithelial Cells - virology Female fluorescence in situ hybridization Follow-Up Studies human papillomavirus Humans In Situ Hybridization, Fluorescence Papillomaviridae - isolation & purification Papillomavirus Infections - virology Sensitivity and Specificity Uterine Cervical Neoplasms - virology Vaginal Smears |
| title | Evaluation of quantity and staining pattern of human papillomavirus (HPV)‐infected epithelial cells in thin‐layer cervical specimens using optimized HPV‐CARD assay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T01%3A19%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20quantity%20and%20staining%20pattern%20of%20human%20papillomavirus%20(HPV)%E2%80%90infected%20epithelial%20cells%20in%20thin%E2%80%90layer%20cervical%20specimens%20using%20optimized%20HPV%E2%80%90CARD%20assay&rft.jtitle=Cancer&rft.au=Algeciras%E2%80%90Schimnich,%20Alicia&rft.date=2007-10-25&rft.volume=111&rft.issue=5&rft.spage=330&rft.epage=338&rft.pages=330-338&rft.issn=0008-543X&rft.eissn=1097-0142&rft_id=info:doi/10.1002/cncr.22946&rft_dat=%3Cproquest_cross%3E68407986%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68407986&rft_id=info:pmid/17724679&rfr_iscdi=true |