High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography with UV detection
A new separation and quantification method using ultra-performance liquid chromatography (UPLC) with UV detection was developed for detection of lincomycin traces in fermentation broth of different Streptomyces spp. A similar high-performance liquid chromatography (HPLC) protocol was simultaneously...
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| Veröffentlicht in: | Journal of chromatography 2007-01, Vol.1139 (2), p.214-220 |
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| container_title | Journal of chromatography |
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| creator | OLSOVSKA, Jana JELINKOVA, Markéta MAN, Petr KOBERSKA, Markéta JANATA, Jiri FLIEGER, Miroslav |
| description | A new separation and quantification method using ultra-performance liquid chromatography (UPLC) with UV detection was developed for detection of lincomycin traces in fermentation broth of different Streptomyces spp. A similar high-performance liquid chromatography (HPLC) protocol was simultaneously developed for comparison purposes. Both methods were validated and showed a linear range of detector response for quantification of lincomycin in concentration from 3.125 to 1000.0 microgml(-1) with correlation coefficient 0.999 and recoveries ranging from 81.5 to 89.85% with precision < or =5%. Compared with the HPLC, the UPLC method offered high sample throughput and about 10 times lower consumption of solvents. The developed assays were used for determination of lincomycin production in genetically manipulated production strain Streptomyces lincolnensis and for determination of lincomycin production after heterologous expression of lincomycin biosynthetic gene cluster in non-producing strain Streptomyces coelicolor. |
| doi_str_mv | 10.1016/j.chroma.2006.11.017 |
| format | Article |
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Both methods were validated and showed a linear range of detector response for quantification of lincomycin in concentration from 3.125 to 1000.0 microgml(-1) with correlation coefficient 0.999 and recoveries ranging from 81.5 to 89.85% with precision < or =5%. Compared with the HPLC, the UPLC method offered high sample throughput and about 10 times lower consumption of solvents. The developed assays were used for determination of lincomycin production in genetically manipulated production strain Streptomyces lincolnensis and for determination of lincomycin production after heterologous expression of lincomycin biosynthetic gene cluster in non-producing strain Streptomyces coelicolor.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2006.11.017</identifier><identifier>PMID: 17125778</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Antibiotics (antibacterial agents, antifungal agents) ; Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents ; Applied microbiology ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Chromatography, Liquid - methods ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Lincomycin - analysis ; Microbiology ; Organisms, Genetically Modified - metabolism ; Sensitivity and Specificity ; Spectrophotometry, Ultraviolet - methods ; Streptomyces - genetics ; Streptomyces - metabolism</subject><ispartof>Journal of chromatography, 2007-01, Vol.1139 (2), p.214-220</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18395930$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17125778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OLSOVSKA, Jana</creatorcontrib><creatorcontrib>JELINKOVA, Markéta</creatorcontrib><creatorcontrib>MAN, Petr</creatorcontrib><creatorcontrib>KOBERSKA, Markéta</creatorcontrib><creatorcontrib>JANATA, Jiri</creatorcontrib><creatorcontrib>FLIEGER, Miroslav</creatorcontrib><title>High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography with UV detection</title><title>Journal of chromatography</title><addtitle>J Chromatogr A</addtitle><description>A new separation and quantification method using ultra-performance liquid chromatography (UPLC) with UV detection was developed for detection of lincomycin traces in fermentation broth of different Streptomyces spp. A similar high-performance liquid chromatography (HPLC) protocol was simultaneously developed for comparison purposes. Both methods were validated and showed a linear range of detector response for quantification of lincomycin in concentration from 3.125 to 1000.0 microgml(-1) with correlation coefficient 0.999 and recoveries ranging from 81.5 to 89.85% with precision < or =5%. Compared with the HPLC, the UPLC method offered high sample throughput and about 10 times lower consumption of solvents. 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Psychology</subject><subject>Lincomycin - analysis</subject><subject>Microbiology</subject><subject>Organisms, Genetically Modified - metabolism</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P1CAYhzlo3HX1GxjDRW-t_GmhHM1EXZNNPOh6ndDyMmVTCgM0Zr6wn0MmM8ajJwh5fg-_FxB6Q0lLCRUfntppTsHrlhEiWkpbQuUzdEsIo40Skt-glzk_kXpKJHuBbqikrJdyuEW_791hbkpNb4c5bgUfN70WZ92kiwsrDhYvbp2CP01uxSXpCTKuOwvJw1ou0JhCmc_oAVYoNbosJ-yDqRow-HtJEMvZUKM5xhbvgo86uXzxb0vVNhGSDcnrdYJ643FzBl9mKuGQdJxPWK8Gz-e2_0d_udrn8Sc2UGA6V3yFnlu9ZHh9Xe_Q4-dPP3b3zcO3L193Hx-ayLgqjRl5R3o6wMhEZzsG0lgNUhMhLVFa2UHxsR-o7TsFAkBawTmQ0QhQHeea36H3F29M4bhBLnvv8gTLolcIW96LgSvGZFfBt1dwGz2YfUzO63Ta__2ZCry7AjrXB7WpjuvyP66KesUJ_wP-M6ZQ</recordid><startdate>20070119</startdate><enddate>20070119</enddate><creator>OLSOVSKA, Jana</creator><creator>JELINKOVA, Markéta</creator><creator>MAN, Petr</creator><creator>KOBERSKA, Markéta</creator><creator>JANATA, Jiri</creator><creator>FLIEGER, Miroslav</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20070119</creationdate><title>High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography with UV detection</title><author>OLSOVSKA, Jana ; JELINKOVA, Markéta ; MAN, Petr ; KOBERSKA, Markéta ; JANATA, Jiri ; FLIEGER, Miroslav</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-db340518eb264f42e7dfae7a067f09a9f893b581f549e6ee7f633e0bd6e9433a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antibiotics (antibacterial agents, antifungal agents)</topic><topic>Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lincomycin - analysis</topic><topic>Microbiology</topic><topic>Organisms, Genetically Modified - metabolism</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OLSOVSKA, Jana</creatorcontrib><creatorcontrib>JELINKOVA, Markéta</creatorcontrib><creatorcontrib>MAN, Petr</creatorcontrib><creatorcontrib>KOBERSKA, Markéta</creatorcontrib><creatorcontrib>JANATA, Jiri</creatorcontrib><creatorcontrib>FLIEGER, Miroslav</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OLSOVSKA, Jana</au><au>JELINKOVA, Markéta</au><au>MAN, Petr</au><au>KOBERSKA, Markéta</au><au>JANATA, Jiri</au><au>FLIEGER, Miroslav</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography with UV detection</atitle><jtitle>Journal of chromatography</jtitle><addtitle>J Chromatogr A</addtitle><date>2007-01-19</date><risdate>2007</risdate><volume>1139</volume><issue>2</issue><spage>214</spage><epage>220</epage><pages>214-220</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A new separation and quantification method using ultra-performance liquid chromatography (UPLC) with UV detection was developed for detection of lincomycin traces in fermentation broth of different Streptomyces spp. A similar high-performance liquid chromatography (HPLC) protocol was simultaneously developed for comparison purposes. Both methods were validated and showed a linear range of detector response for quantification of lincomycin in concentration from 3.125 to 1000.0 microgml(-1) with correlation coefficient 0.999 and recoveries ranging from 81.5 to 89.85% with precision < or =5%. Compared with the HPLC, the UPLC method offered high sample throughput and about 10 times lower consumption of solvents. The developed assays were used for determination of lincomycin production in genetically manipulated production strain Streptomyces lincolnensis and for determination of lincomycin production after heterologous expression of lincomycin biosynthetic gene cluster in non-producing strain Streptomyces coelicolor.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>17125778</pmid><doi>10.1016/j.chroma.2006.11.017</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of chromatography, 2007-01, Vol.1139 (2), p.214-220 |
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| subjects | Antibiotics (antibacterial agents, antifungal agents) Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents Applied microbiology Biological and medical sciences Chromatography, High Pressure Liquid - methods Chromatography, Liquid - methods Fermentation Fundamental and applied biological sciences. Psychology Lincomycin - analysis Microbiology Organisms, Genetically Modified - metabolism Sensitivity and Specificity Spectrophotometry, Ultraviolet - methods Streptomyces - genetics Streptomyces - metabolism |
| title | High-throughput quantification of lincomycin traces in fermentation broth of genetically modified Streptomyces spp. Comparison of ultra-performance liquid chromatography and high-performance liquid chromatography with UV detection |
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