Apoptosis in epithelial ovarian tumours Prognostic significance of clinical and histopathologic factors and its association with the immunohistochemical expression of apoptotic regulatory proteins (p53, bcl-2 and bax)
The objective was to establish the importance of apoptosis-related genes and the apoptotic index (AI) as prognostic factors in patients with epithelial ovarian tumours. Tumour specimens from 130 epithelial ovarian tumours were examined for expression of p53, bcl-2 and bax proteins. The apoptotic ind...
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| Veröffentlicht in: | European journal of obstetrics & gynecology and reproductive biology 2007, Vol.130 (1), p.121-128 |
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| creator | DE LA TORRE, F. Javier GARCIA, Angel GIL-MORENO, Antonio PLANAGUMA, Jesus REVENTOS, Jaume Y CAJAL, Santiago Ramon XERCAVINS, Jordi |
| description | The objective was to establish the importance of apoptosis-related genes and the apoptotic index (AI) as prognostic factors in patients with epithelial ovarian tumours.
Tumour specimens from 130 epithelial ovarian tumours were examined for expression of p53, bcl-2 and bax proteins. The apoptotic index was evaluated by modified terminal deoxynucleotide-transferase-mediated digoxigenin triphosphate-biotin nick end-labelling (TUNEL) methods and by haematoxylin-eosin (H-E) staining. Other clinical and histopathological variables were also analysed. Statistical analyses were performed using the SPSS 10.0 package.
The apoptotic index detected by H-E and enzymatic assay was high in the majority of carcinomas (serous, endometrioid and clear cell (p=0.001). In the univariate survival analysis, high apoptotic index, high p53 and low bcl-2 expression, significantly correlated with shorter survival and shorter disease-free periods (p |
| doi_str_mv | 10.1016/j.ejogrb.2005.11.048 |
| format | Article |
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Tumour specimens from 130 epithelial ovarian tumours were examined for expression of p53, bcl-2 and bax proteins. The apoptotic index was evaluated by modified terminal deoxynucleotide-transferase-mediated digoxigenin triphosphate-biotin nick end-labelling (TUNEL) methods and by haematoxylin-eosin (H-E) staining. Other clinical and histopathological variables were also analysed. Statistical analyses were performed using the SPSS 10.0 package.
The apoptotic index detected by H-E and enzymatic assay was high in the majority of carcinomas (serous, endometrioid and clear cell (p=0.001). In the univariate survival analysis, high apoptotic index, high p53 and low bcl-2 expression, significantly correlated with shorter survival and shorter disease-free periods (p<0.01). In multivariate analysis the age (p=0.026; CI: 1.035-1.066 and p=0.011; CI: 1.835-2.077), FIGO stage (p=0.017; CI: 1.385-4.514 and p=0.024; CI: 1.217-1.940), p53 expression (p=0.03; CI: 1.017-1.627 and p=0.02; CI: 1.050-2.350) and apoptotic index (p=0.019; CI: 1.375-1.750 and p=0.019; CI: 1.442-2.085) were revealed to be independent prognostic factors for survival and recurrence, respectively.
Our results indicate that the apoptotic index and p53 nuclear accumulation are independent predictive factors of recurrence and short survival. Our data also suggest different patterns of alterations for the various tumour types.</description><identifier>ISSN: 0301-2115</identifier><identifier>EISSN: 1872-7654</identifier><identifier>DOI: 10.1016/j.ejogrb.2005.11.048</identifier><identifier>PMID: 16876311</identifier><identifier>CODEN: EOGRAL</identifier><language>eng</language><publisher>Shannon: Elsevier</publisher><subject>Adenocarcinoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Apoptosis ; bcl-2-Associated X Protein - metabolism ; Biological and medical sciences ; Biomarkers ; Cystadenoma, Mucinous - pathology ; Cystadenoma, Serous - pathology ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Kaplan-Meier Estimate ; Medical sciences ; Middle Aged ; Ovarian Neoplasms - pathology ; Predictive Value of Tests ; Prognosis ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Recurrence ; Tumor Suppressor Protein p53 - metabolism ; Tumors</subject><ispartof>European journal of obstetrics & gynecology and reproductive biology, 2007, Vol.130 (1), p.121-128</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18440614$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16876311$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DE LA TORRE, F. Javier</creatorcontrib><creatorcontrib>GARCIA, Angel</creatorcontrib><creatorcontrib>GIL-MORENO, Antonio</creatorcontrib><creatorcontrib>PLANAGUMA, Jesus</creatorcontrib><creatorcontrib>REVENTOS, Jaume</creatorcontrib><creatorcontrib>Y CAJAL, Santiago Ramon</creatorcontrib><creatorcontrib>XERCAVINS, Jordi</creatorcontrib><title>Apoptosis in epithelial ovarian tumours Prognostic significance of clinical and histopathologic factors and its association with the immunohistochemical expression of apoptotic regulatory proteins (p53, bcl-2 and bax)</title><title>European journal of obstetrics & gynecology and reproductive biology</title><addtitle>Eur J Obstet Gynecol Reprod Biol</addtitle><description>The objective was to establish the importance of apoptosis-related genes and the apoptotic index (AI) as prognostic factors in patients with epithelial ovarian tumours.
Tumour specimens from 130 epithelial ovarian tumours were examined for expression of p53, bcl-2 and bax proteins. The apoptotic index was evaluated by modified terminal deoxynucleotide-transferase-mediated digoxigenin triphosphate-biotin nick end-labelling (TUNEL) methods and by haematoxylin-eosin (H-E) staining. Other clinical and histopathological variables were also analysed. Statistical analyses were performed using the SPSS 10.0 package.
The apoptotic index detected by H-E and enzymatic assay was high in the majority of carcinomas (serous, endometrioid and clear cell (p=0.001). In the univariate survival analysis, high apoptotic index, high p53 and low bcl-2 expression, significantly correlated with shorter survival and shorter disease-free periods (p<0.01). In multivariate analysis the age (p=0.026; CI: 1.035-1.066 and p=0.011; CI: 1.835-2.077), FIGO stage (p=0.017; CI: 1.385-4.514 and p=0.024; CI: 1.217-1.940), p53 expression (p=0.03; CI: 1.017-1.627 and p=0.02; CI: 1.050-2.350) and apoptotic index (p=0.019; CI: 1.375-1.750 and p=0.019; CI: 1.442-2.085) were revealed to be independent prognostic factors for survival and recurrence, respectively.
Our results indicate that the apoptotic index and p53 nuclear accumulation are independent predictive factors of recurrence and short survival. Our data also suggest different patterns of alterations for the various tumour types.</description><subject>Adenocarcinoma - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Cystadenoma, Mucinous - pathology</subject><subject>Cystadenoma, Serous - pathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Predictive Value of Tests</subject><subject>Prognosis</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Recurrence</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><subject>Tumors</subject><issn>0301-2115</issn><issn>1872-7654</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkdGO1CAUhonRuLOrb2AMNxpNbIUCnfZys3HVZBO90OsJ0NPOmVCoQHX3UX0bmXGM3JwQvvN_JwdCXnBWc8bb94caDmGKpm4YUzXnNZPdI7Lh3baptq2Sj8mGCcarhnN1QS5TOrByhOifkgvedttWcL4hv6-XsOSQMFH0FBbMe3CoHQ0_dUTtaV7nsMZEv8Yw-ZAyWppw8jii1d4CDSO1Dn25Oar9QPeYclh03gcXpgKP2uZQ-o9vmEtNKVjUGYOnv4qNFiHFeV59OLXaPcynMLhfIqR05IpDn8Y82iNMq9Ml84EuMWRAn-ibRYl31FhXNSeR0fdvn5Eno3YJnp_rFfl---Hbzafq7svHzzfXd9XSiD5XfSuttqAaNdqxM6oR225QUkMPYAbTWiWlHLa9Za2xSjRWMyPsKJXpJLBRiyvy-m9umebHCinvZkwWnNMewpp2bSf6hitWwJdncDUzDLsl4qzjw-7fZxTg1RnQqWxgjGXBmP5znZSs5VL8AYzBom4</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>DE LA TORRE, F. Javier</creator><creator>GARCIA, Angel</creator><creator>GIL-MORENO, Antonio</creator><creator>PLANAGUMA, Jesus</creator><creator>REVENTOS, Jaume</creator><creator>Y CAJAL, Santiago Ramon</creator><creator>XERCAVINS, Jordi</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2007</creationdate><title>Apoptosis in epithelial ovarian tumours Prognostic significance of clinical and histopathologic factors and its association with the immunohistochemical expression of apoptotic regulatory proteins (p53, bcl-2 and bax)</title><author>DE LA TORRE, F. Javier ; GARCIA, Angel ; GIL-MORENO, Antonio ; PLANAGUMA, Jesus ; REVENTOS, Jaume ; Y CAJAL, Santiago Ramon ; XERCAVINS, Jordi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-964cace525fcf8b52378d54ae9eebdb6c5444d79c06bc532ca0b3cf45b84e0fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenocarcinoma - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Apoptosis</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Cystadenoma, Mucinous - pathology</topic><topic>Cystadenoma, Serous - pathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Predictive Value of Tests</topic><topic>Prognosis</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Recurrence</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DE LA TORRE, F. Javier</creatorcontrib><creatorcontrib>GARCIA, Angel</creatorcontrib><creatorcontrib>GIL-MORENO, Antonio</creatorcontrib><creatorcontrib>PLANAGUMA, Jesus</creatorcontrib><creatorcontrib>REVENTOS, Jaume</creatorcontrib><creatorcontrib>Y CAJAL, Santiago Ramon</creatorcontrib><creatorcontrib>XERCAVINS, Jordi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of obstetrics & gynecology and reproductive biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DE LA TORRE, F. Javier</au><au>GARCIA, Angel</au><au>GIL-MORENO, Antonio</au><au>PLANAGUMA, Jesus</au><au>REVENTOS, Jaume</au><au>Y CAJAL, Santiago Ramon</au><au>XERCAVINS, Jordi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis in epithelial ovarian tumours Prognostic significance of clinical and histopathologic factors and its association with the immunohistochemical expression of apoptotic regulatory proteins (p53, bcl-2 and bax)</atitle><jtitle>European journal of obstetrics & gynecology and reproductive biology</jtitle><addtitle>Eur J Obstet Gynecol Reprod Biol</addtitle><date>2007</date><risdate>2007</risdate><volume>130</volume><issue>1</issue><spage>121</spage><epage>128</epage><pages>121-128</pages><issn>0301-2115</issn><eissn>1872-7654</eissn><coden>EOGRAL</coden><abstract>The objective was to establish the importance of apoptosis-related genes and the apoptotic index (AI) as prognostic factors in patients with epithelial ovarian tumours.
Tumour specimens from 130 epithelial ovarian tumours were examined for expression of p53, bcl-2 and bax proteins. The apoptotic index was evaluated by modified terminal deoxynucleotide-transferase-mediated digoxigenin triphosphate-biotin nick end-labelling (TUNEL) methods and by haematoxylin-eosin (H-E) staining. Other clinical and histopathological variables were also analysed. Statistical analyses were performed using the SPSS 10.0 package.
The apoptotic index detected by H-E and enzymatic assay was high in the majority of carcinomas (serous, endometrioid and clear cell (p=0.001). In the univariate survival analysis, high apoptotic index, high p53 and low bcl-2 expression, significantly correlated with shorter survival and shorter disease-free periods (p<0.01). In multivariate analysis the age (p=0.026; CI: 1.035-1.066 and p=0.011; CI: 1.835-2.077), FIGO stage (p=0.017; CI: 1.385-4.514 and p=0.024; CI: 1.217-1.940), p53 expression (p=0.03; CI: 1.017-1.627 and p=0.02; CI: 1.050-2.350) and apoptotic index (p=0.019; CI: 1.375-1.750 and p=0.019; CI: 1.442-2.085) were revealed to be independent prognostic factors for survival and recurrence, respectively.
Our results indicate that the apoptotic index and p53 nuclear accumulation are independent predictive factors of recurrence and short survival. Our data also suggest different patterns of alterations for the various tumour types.</abstract><cop>Shannon</cop><pub>Elsevier</pub><pmid>16876311</pmid><doi>10.1016/j.ejogrb.2005.11.048</doi><tpages>8</tpages></addata></record> |
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| subjects | Adenocarcinoma - pathology Adult Aged Aged, 80 and over Apoptosis bcl-2-Associated X Protein - metabolism Biological and medical sciences Biomarkers Cystadenoma, Mucinous - pathology Cystadenoma, Serous - pathology Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Kaplan-Meier Estimate Medical sciences Middle Aged Ovarian Neoplasms - pathology Predictive Value of Tests Prognosis Proto-Oncogene Proteins c-bcl-2 - metabolism Recurrence Tumor Suppressor Protein p53 - metabolism Tumors |
| title | Apoptosis in epithelial ovarian tumours Prognostic significance of clinical and histopathologic factors and its association with the immunohistochemical expression of apoptotic regulatory proteins (p53, bcl-2 and bax) |
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