Enhanced Effect of Fluorescent Whitening Agent on Peroral Infection for Recombinant Baculovirus in the Host Bombyx mori L
The low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (Bombyx mori L). In this study, the effects of a fluorescent whitening agent on improving the oral infection for the...
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| Veröffentlicht in: | Current microbiology 2007, Vol.54 (1), p.5-8 |
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| creator | Wang, Bing Shang, Jinyan Liu, Xunli Cui, Weizheng Wu, Xiaofeng Zhao, Na |
| description | The low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (Bombyx mori L). In this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant Bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. The results showed that the peroral infection can be remarkably enhanced by adding VBL into the larval artificial diet. The maximum infection rate reached as high as 90% with the concentration of VBL (1%), which was then considered as optimal. The total protease activity and pH value of the larval intestinal juice were found to be lower when compared to the control, indicating an abnormal physiological change of the larval digestive system by VBL, which, in turn, resulted in improved peroral infection of recombinant virus. |
| doi_str_mv | 10.1007/s00284-005-0404-4 |
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In this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant Bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. The results showed that the peroral infection can be remarkably enhanced by adding VBL into the larval artificial diet. The maximum infection rate reached as high as 90% with the concentration of VBL (1%), which was then considered as optimal. The total protease activity and pH value of the larval intestinal juice were found to be lower when compared to the control, indicating an abnormal physiological change of the larval digestive system by VBL, which, in turn, resulted in improved peroral infection of recombinant virus.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-005-0404-4</identifier><identifier>PMID: 17160363</identifier><language>eng</language><publisher>United States: New York : Springer-Verlag</publisher><subject>6-Phytase - metabolism ; Administration, Oral ; Animals ; Bacteria ; Baculovirus ; Benzene Derivatives - pharmacology ; Bombyx - immunology ; Bombyx - virology ; Bombyx mori ; Bombyx mori NPV ; Digestive system ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - pharmacology ; Hydrogen-Ion Concentration ; Intestines - immunology ; Larva - immunology ; Larva - virology ; Microbiology ; Mouth - virology ; Nuclear polyhedrosis virus ; Nucleopolyhedrovirus - growth & development ; Proteins ; Recombinant Proteins - biosynthesis</subject><ispartof>Current microbiology, 2007, Vol.54 (1), p.5-8</ispartof><rights>Springer Science+Business Media, Inc. 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-e00e63514d72e3f3a5dd4c39067d16a3172cbeaf9ac9c2085d31913dc7453a413</citedby><cites>FETCH-LOGICAL-c414t-e00e63514d72e3f3a5dd4c39067d16a3172cbeaf9ac9c2085d31913dc7453a413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17160363$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Bing</creatorcontrib><creatorcontrib>Shang, Jinyan</creatorcontrib><creatorcontrib>Liu, Xunli</creatorcontrib><creatorcontrib>Cui, Weizheng</creatorcontrib><creatorcontrib>Wu, Xiaofeng</creatorcontrib><creatorcontrib>Zhao, Na</creatorcontrib><title>Enhanced Effect of Fluorescent Whitening Agent on Peroral Infection for Recombinant Baculovirus in the Host Bombyx mori L</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (Bombyx mori L). In this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant Bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. The results showed that the peroral infection can be remarkably enhanced by adding VBL into the larval artificial diet. The maximum infection rate reached as high as 90% with the concentration of VBL (1%), which was then considered as optimal. The total protease activity and pH value of the larval intestinal juice were found to be lower when compared to the control, indicating an abnormal physiological change of the larval digestive system by VBL, which, in turn, resulted in improved peroral infection of recombinant virus.</description><subject>6-Phytase - metabolism</subject><subject>Administration, Oral</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Baculovirus</subject><subject>Benzene Derivatives - pharmacology</subject><subject>Bombyx - immunology</subject><subject>Bombyx - virology</subject><subject>Bombyx mori</subject><subject>Bombyx mori NPV</subject><subject>Digestive system</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intestines - immunology</subject><subject>Larva - immunology</subject><subject>Larva - virology</subject><subject>Microbiology</subject><subject>Mouth - virology</subject><subject>Nuclear polyhedrosis virus</subject><subject>Nucleopolyhedrovirus - 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metabolism</topic><topic>Administration, Oral</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Baculovirus</topic><topic>Benzene Derivatives - pharmacology</topic><topic>Bombyx - immunology</topic><topic>Bombyx - virology</topic><topic>Bombyx mori</topic><topic>Bombyx mori NPV</topic><topic>Digestive system</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent Dyes - pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intestines - immunology</topic><topic>Larva - immunology</topic><topic>Larva - virology</topic><topic>Microbiology</topic><topic>Mouth - virology</topic><topic>Nuclear polyhedrosis virus</topic><topic>Nucleopolyhedrovirus - growth & development</topic><topic>Proteins</topic><topic>Recombinant Proteins - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Bing</creatorcontrib><creatorcontrib>Shang, Jinyan</creatorcontrib><creatorcontrib>Liu, Xunli</creatorcontrib><creatorcontrib>Cui, Weizheng</creatorcontrib><creatorcontrib>Wu, Xiaofeng</creatorcontrib><creatorcontrib>Zhao, Na</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Bing</au><au>Shang, Jinyan</au><au>Liu, Xunli</au><au>Cui, Weizheng</au><au>Wu, Xiaofeng</au><au>Zhao, Na</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Effect of Fluorescent Whitening Agent on Peroral Infection for Recombinant Baculovirus in the Host Bombyx mori L</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>2007</date><risdate>2007</risdate><volume>54</volume><issue>1</issue><spage>5</spage><epage>8</epage><pages>5-8</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>The low efficiency of the oral infectivity of recombinant polyhedrin-negative baculovirus is a major bottleneck in the application of the baculovirus expression system in the silkworm (Bombyx mori L). In this study, the effects of a fluorescent whitening agent on improving the oral infection for the recombinant Bombyx mori nuclear polyhedrosis virus in silkworm larva and their possible mechanism were investigated. The results showed that the peroral infection can be remarkably enhanced by adding VBL into the larval artificial diet. The maximum infection rate reached as high as 90% with the concentration of VBL (1%), which was then considered as optimal. The total protease activity and pH value of the larval intestinal juice were found to be lower when compared to the control, indicating an abnormal physiological change of the larval digestive system by VBL, which, in turn, resulted in improved peroral infection of recombinant virus.</abstract><cop>United States</cop><pub>New York : Springer-Verlag</pub><pmid>17160363</pmid><doi>10.1007/s00284-005-0404-4</doi><tpages>4</tpages></addata></record> |
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| ispartof | Current microbiology, 2007, Vol.54 (1), p.5-8 |
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| subjects | 6-Phytase - metabolism Administration, Oral Animals Bacteria Baculovirus Benzene Derivatives - pharmacology Bombyx - immunology Bombyx - virology Bombyx mori Bombyx mori NPV Digestive system Fluorescent Dyes - chemistry Fluorescent Dyes - pharmacology Hydrogen-Ion Concentration Intestines - immunology Larva - immunology Larva - virology Microbiology Mouth - virology Nuclear polyhedrosis virus Nucleopolyhedrovirus - growth & development Proteins Recombinant Proteins - biosynthesis |
| title | Enhanced Effect of Fluorescent Whitening Agent on Peroral Infection for Recombinant Baculovirus in the Host Bombyx mori L |
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