Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/SVneo: effect of steroids, flavonoids and NSAIDs
Vitamin C plays an important role in embryogenesis and fetal growth as well as in the progression of pregnancy and delivery. Therefore, it is important to understand the mechanism that mediates its transport to the fetus as well as the possible influences by endogenous and exogenous substances on it...
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| Veröffentlicht in: | Molecular human reproduction 2007-01, Vol.13 (1), p.77-83 |
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| creator | Biondi, C. Pavan, B. Dalpiaz, A. Medici, S. Lunghi, L. Vesce, F. |
| description | Vitamin C plays an important role in embryogenesis and fetal growth as well as in the progression of pregnancy and delivery. Therefore, it is important to understand the mechanism that mediates its transport to the fetus as well as the possible influences by endogenous and exogenous substances on its placental uptake. The aim of this study was to investigate placental sodium-dependent vitamin C transporters (SVCT) 1 and 2. By means of RT–PCR, we found that SVCT2, but not SVCT1, mRNA is expressed in human trophoblast cell line HTR-8/SVneo. Our method was able to confirm SVCT2 mRNA expression in human first-trimester chorionic villi but not in term placental tissue. Cell line kinetic studies of [14C] ascorbic acid (AA) uptake indicated a one-site model and a saturable process. Fetal bovine serum (FBS) and epidermal growth factor (EGF) do not influence the transport properties, although they significantly increase the expression of SVCT2. Steroid hormones (17β-estradiol, progesterone and cortisol), flavonoids (genistein and quercetin) and non-steroidal anti-inflammatory drugs (NSAIDs) (indomethacin and diclofenac) inhibit [14C]AA uptake in a dose-dependent and non-competitive manner. On the contrary, the process is not influenced by aspirin. Our study suggests the use of HTR-8/SVneo cells as a suitable model for trophoblast vitamin C transport investigation. |
| doi_str_mv | 10.1093/molehr/gal092 |
| format | Article |
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Therefore, it is important to understand the mechanism that mediates its transport to the fetus as well as the possible influences by endogenous and exogenous substances on its placental uptake. The aim of this study was to investigate placental sodium-dependent vitamin C transporters (SVCT) 1 and 2. By means of RT–PCR, we found that SVCT2, but not SVCT1, mRNA is expressed in human trophoblast cell line HTR-8/SVneo. Our method was able to confirm SVCT2 mRNA expression in human first-trimester chorionic villi but not in term placental tissue. Cell line kinetic studies of [14C] ascorbic acid (AA) uptake indicated a one-site model and a saturable process. Fetal bovine serum (FBS) and epidermal growth factor (EGF) do not influence the transport properties, although they significantly increase the expression of SVCT2. Steroid hormones (17β-estradiol, progesterone and cortisol), flavonoids (genistein and quercetin) and non-steroidal anti-inflammatory drugs (NSAIDs) (indomethacin and diclofenac) inhibit [14C]AA uptake in a dose-dependent and non-competitive manner. On the contrary, the process is not influenced by aspirin. Our study suggests the use of HTR-8/SVneo cells as a suitable model for trophoblast vitamin C transport investigation.</description><identifier>ISSN: 1360-9947</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/gal092</identifier><identifier>PMID: 17092984</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; ascorbate ; Ascorbic Acid - pharmacokinetics ; Biological and medical sciences ; Carbon Radioisotopes - pharmacokinetics ; Cell Line ; Embryology: invertebrates and vertebrates. Teratology ; Female ; flavonoids ; Flavonoids - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Humans ; Models, Biological ; NSAIDs ; Organic Anion Transporters, Sodium-Dependent - genetics ; Organic Anion Transporters, Sodium-Dependent - metabolism ; Pregnancy ; Pregnancy Trimester, First - metabolism ; RNA, Messenger - metabolism ; Sodium-Coupled Vitamin C Transporters ; steroids ; Steroids - pharmacology ; Symporters - genetics ; Symporters - metabolism ; Term Birth - metabolism ; trophoblasts ; Trophoblasts - metabolism</subject><ispartof>Molecular human reproduction, 2007-01, Vol.13 (1), p.77-83</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Jan 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-6bc73ebb4ad758a6703a4f535ab83baa062fc61440139d8eec1320b0361c70a13</citedby><cites>FETCH-LOGICAL-c491t-6bc73ebb4ad758a6703a4f535ab83baa062fc61440139d8eec1320b0361c70a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18544999$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17092984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Biondi, C.</creatorcontrib><creatorcontrib>Pavan, B.</creatorcontrib><creatorcontrib>Dalpiaz, A.</creatorcontrib><creatorcontrib>Medici, S.</creatorcontrib><creatorcontrib>Lunghi, L.</creatorcontrib><creatorcontrib>Vesce, F.</creatorcontrib><title>Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/SVneo: effect of steroids, flavonoids and NSAIDs</title><title>Molecular human reproduction</title><addtitle>Mol. Hum. Reprod</addtitle><description>Vitamin C plays an important role in embryogenesis and fetal growth as well as in the progression of pregnancy and delivery. Therefore, it is important to understand the mechanism that mediates its transport to the fetus as well as the possible influences by endogenous and exogenous substances on its placental uptake. The aim of this study was to investigate placental sodium-dependent vitamin C transporters (SVCT) 1 and 2. By means of RT–PCR, we found that SVCT2, but not SVCT1, mRNA is expressed in human trophoblast cell line HTR-8/SVneo. Our method was able to confirm SVCT2 mRNA expression in human first-trimester chorionic villi but not in term placental tissue. Cell line kinetic studies of [14C] ascorbic acid (AA) uptake indicated a one-site model and a saturable process. Fetal bovine serum (FBS) and epidermal growth factor (EGF) do not influence the transport properties, although they significantly increase the expression of SVCT2. Steroid hormones (17β-estradiol, progesterone and cortisol), flavonoids (genistein and quercetin) and non-steroidal anti-inflammatory drugs (NSAIDs) (indomethacin and diclofenac) inhibit [14C]AA uptake in a dose-dependent and non-competitive manner. On the contrary, the process is not influenced by aspirin. Our study suggests the use of HTR-8/SVneo cells as a suitable model for trophoblast vitamin C transport investigation.</description><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>ascorbate</subject><subject>Ascorbic Acid - pharmacokinetics</subject><subject>Biological and medical sciences</subject><subject>Carbon Radioisotopes - pharmacokinetics</subject><subject>Cell Line</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>flavonoids</subject><subject>Flavonoids - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Models, Biological</subject><subject>NSAIDs</subject><subject>Organic Anion Transporters, Sodium-Dependent - genetics</subject><subject>Organic Anion Transporters, Sodium-Dependent - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Sodium-Coupled Vitamin C Transporters</subject><subject>steroids</subject><subject>Steroids - pharmacology</subject><subject>Symporters - genetics</subject><subject>Symporters - metabolism</subject><subject>Term Birth - metabolism</subject><subject>trophoblasts</subject><subject>Trophoblasts - metabolism</subject><issn>1360-9947</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1u1DAUhSMEoqWwZIssJFgRxo6d2GZXhdJpVUCiBaHZWDeOQ1ySONhJVfoYPHEdZsRIrHx07-f7d5LkOcFvCZZ01bvOtH71AzosswfJIWEFTjOG-cOoadRSMn6QPAnhGmPCs1w8Tg4Ij7AU7DD5c3I7ehOCdQOCoUa6BQ96Mt7ewbQEXYNu7AS9HVCJJg9DGJ2PeRQDU2tQO_cQlXdj66oOwoS06TrU2cGg9dWXVKwuvw3GvUOmaYyelnohfne2Dm9Q08GNGxb9t_mny-Oz9-Fp8qiBLphnu_co-frh5KpcpxefT8_K44tUM0mmtKg0p6aqGNQ8F1BwTIE1Oc2hErQCwEXW6IIwhgmVtTBGE5rhCtOCaI6B0KPk9bbu6N2v2YRJ9TYsw0Ocdw6qEJTnnPAIvvwPvHazH-JsKsvyDGeC0wilW0h7F4I3jRq97cH_VgSrxSm1dUptnYr8i13RuepNvad31kTg1Q6AoKFr4um1DXtO5IxJKfeNbbzr7b88-J-q4HEDtf6-UaUoN-Lj-lxt6D09zq4q</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Biondi, C.</creator><creator>Pavan, B.</creator><creator>Dalpiaz, A.</creator><creator>Medici, S.</creator><creator>Lunghi, L.</creator><creator>Vesce, F.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/SVneo: effect of steroids, flavonoids and NSAIDs</title><author>Biondi, C. ; Pavan, B. ; Dalpiaz, A. ; Medici, S. ; Lunghi, L. ; Vesce, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-6bc73ebb4ad758a6703a4f535ab83baa062fc61440139d8eec1320b0361c70a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>ascorbate</topic><topic>Ascorbic Acid - pharmacokinetics</topic><topic>Biological and medical sciences</topic><topic>Carbon Radioisotopes - pharmacokinetics</topic><topic>Cell Line</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>flavonoids</topic><topic>Flavonoids - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Models, Biological</topic><topic>NSAIDs</topic><topic>Organic Anion Transporters, Sodium-Dependent - genetics</topic><topic>Organic Anion Transporters, Sodium-Dependent - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Sodium-Coupled Vitamin C Transporters</topic><topic>steroids</topic><topic>Steroids - pharmacology</topic><topic>Symporters - genetics</topic><topic>Symporters - metabolism</topic><topic>Term Birth - metabolism</topic><topic>trophoblasts</topic><topic>Trophoblasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Biondi, C.</creatorcontrib><creatorcontrib>Pavan, B.</creatorcontrib><creatorcontrib>Dalpiaz, A.</creatorcontrib><creatorcontrib>Medici, S.</creatorcontrib><creatorcontrib>Lunghi, L.</creatorcontrib><creatorcontrib>Vesce, F.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Biondi, C.</au><au>Pavan, B.</au><au>Dalpiaz, A.</au><au>Medici, S.</au><au>Lunghi, L.</au><au>Vesce, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/SVneo: effect of steroids, flavonoids and NSAIDs</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol. Hum. Reprod</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>13</volume><issue>1</issue><spage>77</spage><epage>83</epage><pages>77-83</pages><issn>1360-9947</issn><eissn>1460-2407</eissn><abstract>Vitamin C plays an important role in embryogenesis and fetal growth as well as in the progression of pregnancy and delivery. Therefore, it is important to understand the mechanism that mediates its transport to the fetus as well as the possible influences by endogenous and exogenous substances on its placental uptake. The aim of this study was to investigate placental sodium-dependent vitamin C transporters (SVCT) 1 and 2. By means of RT–PCR, we found that SVCT2, but not SVCT1, mRNA is expressed in human trophoblast cell line HTR-8/SVneo. Our method was able to confirm SVCT2 mRNA expression in human first-trimester chorionic villi but not in term placental tissue. Cell line kinetic studies of [14C] ascorbic acid (AA) uptake indicated a one-site model and a saturable process. Fetal bovine serum (FBS) and epidermal growth factor (EGF) do not influence the transport properties, although they significantly increase the expression of SVCT2. Steroid hormones (17β-estradiol, progesterone and cortisol), flavonoids (genistein and quercetin) and non-steroidal anti-inflammatory drugs (NSAIDs) (indomethacin and diclofenac) inhibit [14C]AA uptake in a dose-dependent and non-competitive manner. On the contrary, the process is not influenced by aspirin. Our study suggests the use of HTR-8/SVneo cells as a suitable model for trophoblast vitamin C transport investigation.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>17092984</pmid><doi>10.1093/molehr/gal092</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular human reproduction, 2007-01, Vol.13 (1), p.77-83 |
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| subjects | Anti-Inflammatory Agents, Non-Steroidal - pharmacology ascorbate Ascorbic Acid - pharmacokinetics Biological and medical sciences Carbon Radioisotopes - pharmacokinetics Cell Line Embryology: invertebrates and vertebrates. Teratology Female flavonoids Flavonoids - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Humans Models, Biological NSAIDs Organic Anion Transporters, Sodium-Dependent - genetics Organic Anion Transporters, Sodium-Dependent - metabolism Pregnancy Pregnancy Trimester, First - metabolism RNA, Messenger - metabolism Sodium-Coupled Vitamin C Transporters steroids Steroids - pharmacology Symporters - genetics Symporters - metabolism Term Birth - metabolism trophoblasts Trophoblasts - metabolism |
| title | Expression and characterization of vitamin C transporter in the human trophoblast cell line HTR-8/SVneo: effect of steroids, flavonoids and NSAIDs |
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