Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis
Giardia intestinalis is a flagellated protozoan which causes enteric disease worldwide. Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogen...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 2007-11, Vol.51 (2), p.302-309 |
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| creator | Panaro, Maria Antonietta Cianciulli, Antonia Mitolo, Vincenzo Mitolo, Carlo Ivan Acquafredda, Angela Brandonisio, Olga Cavallo, Pasqua |
| description | Giardia intestinalis is a flagellated protozoan which causes enteric disease worldwide. Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogens, including protozoan parasites, are able to induce enterocyte apoptosis. The aim of this work was to assess whether G. intestinalis strain WB clone C6 is able to induce apoptosis in the human intestinal epithelial cell line HCT-8, and to investigate the role of caspases in this process. Results demonstrated that the parasite induces cell apoptosis, as confirmed by DNA fragmentation analysis, detection of active caspase-3 and degradation of the caspase-3 substrate PARP [poly(ADP-ribose) polymerase]. Furthermore, G. intestinalis infection induces activation of both the intrinsic and the extrinsic apoptotic pathways, down-regulation of the antiapoptotic protein Bcl-2 and up-regulation of the proapoptotic Bax, suggesting a possible role for caspase-dependent apoptosis in the pathogenesis of giardiasis. |
| doi_str_mv | 10.1111/j.1574-695X.2007.00304.x |
| format | Article |
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Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogens, including protozoan parasites, are able to induce enterocyte apoptosis. The aim of this work was to assess whether G. intestinalis strain WB clone C6 is able to induce apoptosis in the human intestinal epithelial cell line HCT-8, and to investigate the role of caspases in this process. Results demonstrated that the parasite induces cell apoptosis, as confirmed by DNA fragmentation analysis, detection of active caspase-3 and degradation of the caspase-3 substrate PARP [poly(ADP-ribose) polymerase]. Furthermore, G. intestinalis infection induces activation of both the intrinsic and the extrinsic apoptotic pathways, down-regulation of the antiapoptotic protein Bcl-2 and up-regulation of the proapoptotic Bax, suggesting a possible role for caspase-dependent apoptosis in the pathogenesis of giardiasis.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.2007.00304.x</identifier><identifier>PMID: 17714487</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Adenosine diphosphate ; Animals ; Apoptosis ; BAX protein ; Bcl-2 protein ; bcl-2-Associated X Protein - biosynthesis ; Biological and medical sciences ; Biotechnology ; caspase ; Caspase 3 - metabolism ; Caspase-3 ; Cell Line, Tumor ; Diarrhea ; DNA Fragmentation ; Epithelial cells ; Epithelial Cells - parasitology ; Gene Expression Regulation ; Giardia ; Giardia intestinalis ; Giardia lamblia ; Giardia lamblia - physiology ; Giardiasis ; HCT-8 cell ; Human protozoal diseases ; Humans ; Infectious diseases ; Intestine ; Medical sciences ; Parasites ; Parasitic diseases ; Parasitic diseases due to ciliates and flagellates ; Pathogenesis ; Poly(ADP-ribose) ; Poly(ADP-ribose) polymerase ; Poly(ADP-ribose) Polymerases - metabolism ; Proto-Oncogene Proteins c-bcl-2 - biosynthesis ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Protozoa ; Protozoal diseases ; Ribose ; Small intestine ; Substrates ; Trophozoites</subject><ispartof>FEMS immunology and medical microbiology, 2007-11, Vol.51 (2), p.302-309</ispartof><rights>2007 Federation of European Microbiological Societies 2007</rights><rights>2007 INIST-CNRS</rights><rights>2007 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5234-310963e0c44afa67f9162a9e861ec82f5b6460485c106bcf57205c6343d2ebd03</citedby><cites>FETCH-LOGICAL-c5234-310963e0c44afa67f9162a9e861ec82f5b6460485c106bcf57205c6343d2ebd03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.2007.00304.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.2007.00304.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19132389$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17714487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panaro, Maria Antonietta</creatorcontrib><creatorcontrib>Cianciulli, Antonia</creatorcontrib><creatorcontrib>Mitolo, Vincenzo</creatorcontrib><creatorcontrib>Mitolo, Carlo Ivan</creatorcontrib><creatorcontrib>Acquafredda, Angela</creatorcontrib><creatorcontrib>Brandonisio, Olga</creatorcontrib><creatorcontrib>Cavallo, Pasqua</creatorcontrib><title>Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis</title><title>FEMS immunology and medical microbiology</title><addtitle>FEMS Immunol Med Microbiol</addtitle><description>Giardia intestinalis is a flagellated protozoan which causes enteric disease worldwide. Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogens, including protozoan parasites, are able to induce enterocyte apoptosis. The aim of this work was to assess whether G. intestinalis strain WB clone C6 is able to induce apoptosis in the human intestinal epithelial cell line HCT-8, and to investigate the role of caspases in this process. Results demonstrated that the parasite induces cell apoptosis, as confirmed by DNA fragmentation analysis, detection of active caspase-3 and degradation of the caspase-3 substrate PARP [poly(ADP-ribose) polymerase]. Furthermore, G. intestinalis infection induces activation of both the intrinsic and the extrinsic apoptotic pathways, down-regulation of the antiapoptotic protein Bcl-2 and up-regulation of the proapoptotic Bax, suggesting a possible role for caspase-dependent apoptosis in the pathogenesis of giardiasis.</description><subject>Adenosine diphosphate</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>bcl-2-Associated X Protein - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>caspase</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase-3</subject><subject>Cell Line, Tumor</subject><subject>Diarrhea</subject><subject>DNA Fragmentation</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - parasitology</subject><subject>Gene Expression Regulation</subject><subject>Giardia</subject><subject>Giardia intestinalis</subject><subject>Giardia lamblia</subject><subject>Giardia lamblia - physiology</subject><subject>Giardiasis</subject><subject>HCT-8 cell</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Intestine</subject><subject>Medical sciences</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitic diseases due to ciliates and flagellates</subject><subject>Pathogenesis</subject><subject>Poly(ADP-ribose)</subject><subject>Poly(ADP-ribose) polymerase</subject><subject>Poly(ADP-ribose) Polymerases - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2 - biosynthesis</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Ribose</subject><subject>Small intestine</subject><subject>Substrates</subject><subject>Trophozoites</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV2L1DAYRoMo7rj6FzQgetf65rMJeLMM7geseOEueBfSNNUMnbYmLe78e9Pt4IIompsk5JzkCQ9CmEBJ8ni3K4moeCG1-FJSgKoEYMDLu0do8-vgMdqApqpQlPMT9CylHQBwDfAUnZCqIpyraoN2W5tGm3zR-NH3je8nbMdhnIYUEh5aPH3z-HJ7Uyjsx5A3XbAddr7rcBd6j0PfzM43uD7ck6ONNoXJ44tgYxNsPp98mkJvu5Ceoyet7ZJ_cZxP0e35h5vtZXH96eJqe3ZdOEEZLxgBLZkHx7ltraxaTSS12itJvFO0FbXkErgSjoCsXSsqCsJJxllDfd0AO0Vv13vHOHyf8_NmH9IS2fZ-mJORii0K_SdICbBKaJ3B17-Bu2GO-VOZYSAZ5VqwTKmVcnFIKfrWjDHsbTwYAmapzezM0o5Z2jFLbea-NnOX1ZfHB-Z675sH8dhTBt4cAZuc7dpoexfSA6cJo0wtSd-v3I_Q-cN_BzDnVx_zIuts1Yd5_Itc_Cn9q9Vq7WDs15iD3X6mQBiAIrQSiv0Ep23KAg</recordid><startdate>200711</startdate><enddate>200711</enddate><creator>Panaro, Maria Antonietta</creator><creator>Cianciulli, Antonia</creator><creator>Mitolo, Vincenzo</creator><creator>Mitolo, Carlo Ivan</creator><creator>Acquafredda, Angela</creator><creator>Brandonisio, Olga</creator><creator>Cavallo, Pasqua</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200711</creationdate><title>Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis</title><author>Panaro, Maria Antonietta ; Cianciulli, Antonia ; Mitolo, Vincenzo ; Mitolo, Carlo Ivan ; Acquafredda, Angela ; Brandonisio, Olga ; Cavallo, Pasqua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5234-310963e0c44afa67f9162a9e861ec82f5b6460485c106bcf57205c6343d2ebd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenosine diphosphate</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>bcl-2-Associated X Protein - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>caspase</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase-3</topic><topic>Cell Line, Tumor</topic><topic>Diarrhea</topic><topic>DNA Fragmentation</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - parasitology</topic><topic>Gene Expression Regulation</topic><topic>Giardia</topic><topic>Giardia intestinalis</topic><topic>Giardia lamblia</topic><topic>Giardia lamblia - physiology</topic><topic>Giardiasis</topic><topic>HCT-8 cell</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Intestine</topic><topic>Medical sciences</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Parasitic diseases due to ciliates and flagellates</topic><topic>Pathogenesis</topic><topic>Poly(ADP-ribose)</topic><topic>Poly(ADP-ribose) polymerase</topic><topic>Poly(ADP-ribose) Polymerases - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2 - biosynthesis</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><topic>Ribose</topic><topic>Small intestine</topic><topic>Substrates</topic><topic>Trophozoites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panaro, Maria Antonietta</creatorcontrib><creatorcontrib>Cianciulli, Antonia</creatorcontrib><creatorcontrib>Mitolo, Vincenzo</creatorcontrib><creatorcontrib>Mitolo, Carlo Ivan</creatorcontrib><creatorcontrib>Acquafredda, Angela</creatorcontrib><creatorcontrib>Brandonisio, Olga</creatorcontrib><creatorcontrib>Cavallo, Pasqua</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panaro, Maria Antonietta</au><au>Cianciulli, Antonia</au><au>Mitolo, Vincenzo</au><au>Mitolo, Carlo Ivan</au><au>Acquafredda, Angela</au><au>Brandonisio, Olga</au><au>Cavallo, Pasqua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><addtitle>FEMS Immunol Med Microbiol</addtitle><date>2007-11</date><risdate>2007</risdate><volume>51</volume><issue>2</issue><spage>302</spage><epage>309</epage><pages>302-309</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Giardia intestinalis is a flagellated protozoan which causes enteric disease worldwide. Giardia trophozoites infect epithelial cells of the proximal small intestine and can cause acute or chronic diarrhea. The mechanism of epithelial injury in giardiasis remains unknown. A number of enteric pathogens, including protozoan parasites, are able to induce enterocyte apoptosis. The aim of this work was to assess whether G. intestinalis strain WB clone C6 is able to induce apoptosis in the human intestinal epithelial cell line HCT-8, and to investigate the role of caspases in this process. Results demonstrated that the parasite induces cell apoptosis, as confirmed by DNA fragmentation analysis, detection of active caspase-3 and degradation of the caspase-3 substrate PARP [poly(ADP-ribose) polymerase]. Furthermore, G. intestinalis infection induces activation of both the intrinsic and the extrinsic apoptotic pathways, down-regulation of the antiapoptotic protein Bcl-2 and up-regulation of the proapoptotic Bax, suggesting a possible role for caspase-dependent apoptosis in the pathogenesis of giardiasis.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17714487</pmid><doi>10.1111/j.1574-695X.2007.00304.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Adenosine diphosphate Animals Apoptosis BAX protein Bcl-2 protein bcl-2-Associated X Protein - biosynthesis Biological and medical sciences Biotechnology caspase Caspase 3 - metabolism Caspase-3 Cell Line, Tumor Diarrhea DNA Fragmentation Epithelial cells Epithelial Cells - parasitology Gene Expression Regulation Giardia Giardia intestinalis Giardia lamblia Giardia lamblia - physiology Giardiasis HCT-8 cell Human protozoal diseases Humans Infectious diseases Intestine Medical sciences Parasites Parasitic diseases Parasitic diseases due to ciliates and flagellates Pathogenesis Poly(ADP-ribose) Poly(ADP-ribose) polymerase Poly(ADP-ribose) Polymerases - metabolism Proto-Oncogene Proteins c-bcl-2 - biosynthesis Proto-Oncogene Proteins c-bcl-2 - metabolism Protozoa Protozoal diseases Ribose Small intestine Substrates Trophozoites |
| title | Caspase-dependent apoptosis of the HCT-8 epithelial cell line induced by the parasite Giardia intestinalis |
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