Gap junction mediated transport of shRNA between human embryonic stem cells

Gap junction intracellular communication (GJIC) allows the direct transport of small molecules between adjacent cells. We hypothesized that siRNAs in one hESC could inhibit target RNA expression in another hESC via GJIC. We co-cultured green fluorescent protein (GFP)-expressing ENVY hESC with non-GF...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 2007-11, Vol.363 (3), p.610-615
Hauptverfasser:	Wolvetang, Ernst J., Pera, Martin F., Zuckerman, Kenneth S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological Transport Cell Line Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Flow Cytometry Gap junctions Gap Junctions - physiology Gene Expression - drug effects GJIC Glycyrrhetinic Acid - pharmacology Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Human embryonic stem cells Humans Immunohistochemistry Lentivirus Mice Mutation RNA, Small Interfering - genetics RNA, Small Interfering - metabolism shRNA Transfection
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!