Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model
In the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of Leishmania (Viannia) peruviana, Leishmania (Viannia) braziliensis and a hybrid between both species. Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloni...
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| Veröffentlicht in: | Parasitology 2007-01, Vol.134 (1), p.33-39 |
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| creator | DUJARDIN, J.-C. DE DONCKER, S. JACQUET, D. BAÑULS, A.-L. BALAVOINE, M. VAN BOCKSTAELE, D. TIBAYRENC, M. AREVALO, J. LE RAY, D. |
| description | In the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of Leishmania (Viannia) peruviana, Leishmania (Viannia) braziliensis and a hybrid between both species. Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns during in vitro maintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. The resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. No change in total ploidy of the cells was observed by flow cytometry. We discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. Our results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology. |
| doi_str_mv | 10.1017/S0031182006001156 |
| format | Article |
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Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns during in vitro maintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. The resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. No change in total ploidy of the cells was observed by flow cytometry. We discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. Our results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182006001156</identifier><identifier>PMID: 16978449</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Biological and medical sciences ; Chromosomes ; Chromosomes - ultrastructure ; Clone Cells ; Culture Techniques ; Drug resistance ; Environmental conditions ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Genetics ; Genome, Protozoan ; hybrid ; Invertebrates ; karyotype ; Karyotypes ; Karyotyping ; Kinases ; Leishmania (Viannia) peruviana ; Leishmania - chemistry ; Leishmania - cytology ; Leishmania - genetics ; Leishmania - growth & development ; Leishmania braziliensis - cytology ; Leishmania braziliensis - genetics ; Leishmania braziliensis - growth & development ; Life Cycle Stages ; Models, Biological ; Parasites ; Parasitic protozoa ; Ploidies ; ploidy ; Population ; pseudo-sexuality ; Segregation ; selection</subject><ispartof>Parasitology, 2007-01, Vol.134 (1), p.33-39</ispartof><rights>2006 Cambridge University Press</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Cambridge University Press Jan 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-5897e7f844db6d75a62ec4a6edd0c4a03fa369d71fe3effc1454dc4fa63aefa43</citedby><cites>FETCH-LOGICAL-c438t-5897e7f844db6d75a62ec4a6edd0c4a03fa369d71fe3effc1454dc4fa63aefa43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182006001156/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,4024,27923,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18446208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16978449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DUJARDIN, J.-C.</creatorcontrib><creatorcontrib>DE DONCKER, S.</creatorcontrib><creatorcontrib>JACQUET, D.</creatorcontrib><creatorcontrib>BAÑULS, A.-L.</creatorcontrib><creatorcontrib>BALAVOINE, M.</creatorcontrib><creatorcontrib>VAN BOCKSTAELE, D.</creatorcontrib><creatorcontrib>TIBAYRENC, M.</creatorcontrib><creatorcontrib>AREVALO, J.</creatorcontrib><creatorcontrib>LE RAY, D.</creatorcontrib><title>Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>In the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of Leishmania (Viannia) peruviana, Leishmania (Viannia) braziliensis and a hybrid between both species. Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns during in vitro maintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. The resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. No change in total ploidy of the cells was observed by flow cytometry. We discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. Our results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromosomes</subject><subject>Chromosomes - ultrastructure</subject><subject>Clone Cells</subject><subject>Culture Techniques</subject><subject>Drug resistance</subject><subject>Environmental conditions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Genetics</subject><subject>Genome, Protozoan</subject><subject>hybrid</subject><subject>Invertebrates</subject><subject>karyotype</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>Kinases</subject><subject>Leishmania (Viannia) peruviana</subject><subject>Leishmania - chemistry</subject><subject>Leishmania - cytology</subject><subject>Leishmania - genetics</subject><subject>Leishmania - growth & development</subject><subject>Leishmania braziliensis - cytology</subject><subject>Leishmania braziliensis - genetics</subject><subject>Leishmania braziliensis - growth & development</subject><subject>Life Cycle Stages</subject><subject>Models, Biological</subject><subject>Parasites</subject><subject>Parasitic protozoa</subject><subject>Ploidies</subject><subject>ploidy</subject><subject>Population</subject><subject>pseudo-sexuality</subject><subject>Segregation</subject><subject>selection</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp10N9v0zAQB3ALMbEy-AN4QRYSvIXZseMkvE2FDaROMH5MvFnX-Nx5S-JiuxP973HVaJVAPJ3k-5x19yXkBWdvOeP16TfGBOdNyZhijPNKPSIzLlVbNFzxx2S2axe7_jF5GuMty06o8gk55qqtGynbGdHz3o_Q03Xwa1hBcn6kMBqabpBaiImucMSwf_eW3kHY-rRdIzXuHkN0afsue-pGeu9S8HSBLt4MMDqggzfYPyNHFvqIz6d6Qn6cf_g-_1gsPl98mp8tik6KJhVV09ZY27yTWSpTV6BK7CQoNIblyoQFoVpTc4sCre24rKTppAUlAC1IcULe7P_Nh_zaYEx6cLHDvocR_SZq1QhV1aXK8NVf8NZvQo4g6jJnx1shqoz4HnXBxxjQ6nVwQ75dc6Z30et_os8zL6ePN8sBzWFiyjqD1xOA2EFvA4ydiweXkSpZk12xdy4m_P3Qh3CnVS3qSquLK_3l8vrrz-v3V_o8ezEtC8MyOLPCw0n_X_cPEBSq2A</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>DUJARDIN, J.-C.</creator><creator>DE DONCKER, S.</creator><creator>JACQUET, D.</creator><creator>BAÑULS, A.-L.</creator><creator>BALAVOINE, M.</creator><creator>VAN BOCKSTAELE, D.</creator><creator>TIBAYRENC, M.</creator><creator>AREVALO, J.</creator><creator>LE RAY, D.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200701</creationdate><title>Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model</title><author>DUJARDIN, J.-C. ; DE DONCKER, S. ; JACQUET, D. ; BAÑULS, A.-L. ; BALAVOINE, M. ; VAN BOCKSTAELE, D. ; TIBAYRENC, M. ; AREVALO, J. ; LE RAY, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-5897e7f844db6d75a62ec4a6edd0c4a03fa369d71fe3effc1454dc4fa63aefa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromosomes</topic><topic>Chromosomes - ultrastructure</topic><topic>Clone Cells</topic><topic>Culture Techniques</topic><topic>Drug resistance</topic><topic>Environmental conditions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Genetics</topic><topic>Genome, Protozoan</topic><topic>hybrid</topic><topic>Invertebrates</topic><topic>karyotype</topic><topic>Karyotypes</topic><topic>Karyotyping</topic><topic>Kinases</topic><topic>Leishmania (Viannia) peruviana</topic><topic>Leishmania - chemistry</topic><topic>Leishmania - cytology</topic><topic>Leishmania - genetics</topic><topic>Leishmania - growth & development</topic><topic>Leishmania braziliensis - cytology</topic><topic>Leishmania braziliensis - genetics</topic><topic>Leishmania braziliensis - growth & development</topic><topic>Life Cycle Stages</topic><topic>Models, Biological</topic><topic>Parasites</topic><topic>Parasitic protozoa</topic><topic>Ploidies</topic><topic>ploidy</topic><topic>Population</topic><topic>pseudo-sexuality</topic><topic>Segregation</topic><topic>selection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DUJARDIN, J.-C.</creatorcontrib><creatorcontrib>DE DONCKER, S.</creatorcontrib><creatorcontrib>JACQUET, D.</creatorcontrib><creatorcontrib>BAÑULS, A.-L.</creatorcontrib><creatorcontrib>BALAVOINE, M.</creatorcontrib><creatorcontrib>VAN BOCKSTAELE, D.</creatorcontrib><creatorcontrib>TIBAYRENC, M.</creatorcontrib><creatorcontrib>AREVALO, J.</creatorcontrib><creatorcontrib>LE RAY, D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DUJARDIN, J.-C.</au><au>DE DONCKER, S.</au><au>JACQUET, D.</au><au>BAÑULS, A.-L.</au><au>BALAVOINE, M.</au><au>VAN BOCKSTAELE, D.</au><au>TIBAYRENC, M.</au><au>AREVALO, J.</au><au>LE RAY, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2007-01</date><risdate>2007</risdate><volume>134</volume><issue>1</issue><spage>33</spage><epage>39</epage><pages>33-39</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>In the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of Leishmania (Viannia) peruviana, Leishmania (Viannia) braziliensis and a hybrid between both species. Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns during in vitro maintenance were rapid and discrete, involving loss of heteromorphy or appearance of additional chromosome size variants. The resulting pattern was not the same according to experimental conditions (subinoculation rate or incubation temperature), and interestingly, this was associated with differences in growth behaviour of the respective parasites. No change in total ploidy of the cells was observed by flow cytometry. We discuss several mechanisms that might account for this variation of chromosome patterns, but we favour the occurrence of aneuploidy, caused by aberrant chromosome segregation during mitosis. Our results provide insight into the generation of karyotype diversity in natural conditions and highlight the relativity of the clone concept in parasitology.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>16978449</pmid><doi>10.1017/S0031182006001156</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Chromosomes Chromosomes - ultrastructure Clone Cells Culture Techniques Drug resistance Environmental conditions Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Genetics Genome, Protozoan hybrid Invertebrates karyotype Karyotypes Karyotyping Kinases Leishmania (Viannia) peruviana Leishmania - chemistry Leishmania - cytology Leishmania - genetics Leishmania - growth & development Leishmania braziliensis - cytology Leishmania braziliensis - genetics Leishmania braziliensis - growth & development Life Cycle Stages Models, Biological Parasites Parasitic protozoa Ploidies ploidy Population pseudo-sexuality Segregation selection |
| title | Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model |
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