Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function
Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization p...
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| Veröffentlicht in: | Cell 2007-10, Vol.131 (1), p.174-187 |
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| container_title | Cell |
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| creator | Lécuyer, Eric Yoshida, Hideki Parthasarathy, Neela Alm, Christina Babak, Tomas Cerovina, Tanja Hughes, Timothy R. Tomancak, Pavel Krause, Henry M. |
| description | Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization procedure to comprehensively evaluate mRNA localization dynamics during early
Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions. |
| doi_str_mv | 10.1016/j.cell.2007.08.003 |
| format | Article |
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Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2007.08.003</identifier><identifier>PMID: 17923096</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Division - physiology ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; CELLBIO ; Databases, Nucleic Acid ; DEVBIO ; Drosophila ; Drosophila melanogaster - anatomy & histology ; Drosophila melanogaster - embryology ; Drosophila melanogaster - genetics ; Drosophila Proteins - genetics ; Drosophila Proteins - metabolism ; Embryo, Nonmammalian - anatomy & histology ; Embryo, Nonmammalian - physiology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; In Situ Hybridization, Fluorescence - methods ; RNA ; RNA, Messenger - metabolism</subject><ispartof>Cell, 2007-10, Vol.131 (1), p.174-187</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-f4b08eb55ed81b3237a04d44b80769fdce5a65bcb743d58d98d55c4b5c01383d3</citedby><cites>FETCH-LOGICAL-c495t-f4b08eb55ed81b3237a04d44b80769fdce5a65bcb743d58d98d55c4b5c01383d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0092867407010227$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17923096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lécuyer, Eric</creatorcontrib><creatorcontrib>Yoshida, Hideki</creatorcontrib><creatorcontrib>Parthasarathy, Neela</creatorcontrib><creatorcontrib>Alm, Christina</creatorcontrib><creatorcontrib>Babak, Tomas</creatorcontrib><creatorcontrib>Cerovina, Tanja</creatorcontrib><creatorcontrib>Hughes, Timothy R.</creatorcontrib><creatorcontrib>Tomancak, Pavel</creatorcontrib><creatorcontrib>Krause, Henry M.</creatorcontrib><title>Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function</title><title>Cell</title><addtitle>Cell</addtitle><description>Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization procedure to comprehensively evaluate mRNA localization dynamics during early
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A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions.</description><subject>Animals</subject><subject>Cell Division - physiology</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>CELLBIO</subject><subject>Databases, Nucleic Acid</subject><subject>DEVBIO</subject><subject>Drosophila</subject><subject>Drosophila melanogaster - anatomy & histology</subject><subject>Drosophila melanogaster - embryology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins - genetics</subject><subject>Drosophila Proteins - metabolism</subject><subject>Embryo, Nonmammalian - anatomy & histology</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>RNA</subject><subject>RNA, Messenger - metabolism</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURS0EokPhB1ggr9glfY7jxJHYjEZtQRpRNIK15dgvxSPHLnZSqf16Es1I7Ojqbc49erqXkI8MSgasuTqWBr0vK4C2BFkC8Fdkw6Bri5q11WuyAeiqQjZtfUHe5XwEACmEeEsuWNtVHLpmQ8Ktj732dBu0f8ou0zjQ8fB9S_fRaO-e9eRioAd8RO0z1fRHiqMLGCZ6iB6pC_Qu3evgnl24p7vlndnrRLfJ_HYTmmlOSHWw9GYOZjW9J2-GRYQfzveS_Lq5_rn7Wuzvbr_ttvvC1J2YiqHuQWIvBFrJel7xVkNt67qX0DbdYA0K3Yje9G3NrZC2k1YIU_fCAOOSW35JPp-8Dyn-mTFPanR5bUsHjHNWjeQNiIq_CFYL1VSMLWB1Ak2KOScc1ENyo05PioFa51BHtebUOocCqZY5ltCns33uR7T_Iuf-F-DLCcCljEeHSWXjMBi0Li31KRvd__x_AdPonDY</recordid><startdate>20071005</startdate><enddate>20071005</enddate><creator>Lécuyer, Eric</creator><creator>Yoshida, Hideki</creator><creator>Parthasarathy, Neela</creator><creator>Alm, Christina</creator><creator>Babak, Tomas</creator><creator>Cerovina, Tanja</creator><creator>Hughes, Timothy R.</creator><creator>Tomancak, Pavel</creator><creator>Krause, Henry M.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071005</creationdate><title>Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function</title><author>Lécuyer, Eric ; 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Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17923096</pmid><doi>10.1016/j.cell.2007.08.003</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cell Division - physiology Cell Nucleus - metabolism Cell Nucleus - ultrastructure CELLBIO Databases, Nucleic Acid DEVBIO Drosophila Drosophila melanogaster - anatomy & histology Drosophila melanogaster - embryology Drosophila melanogaster - genetics Drosophila Proteins - genetics Drosophila Proteins - metabolism Embryo, Nonmammalian - anatomy & histology Embryo, Nonmammalian - physiology Gene Expression Profiling Gene Expression Regulation, Developmental In Situ Hybridization, Fluorescence - methods RNA RNA, Messenger - metabolism |
| title | Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function |
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