A cytokine immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance spectroscopy
A biosensor for the serum cytokine, interleukin-12 (IL-12), based upon a label-free electrochemical impedance spectroscopy monitoring is described. Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coa...
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| Veröffentlicht in: | Biosensors & bioelectronics 2007-10, Vol.23 (3), p.428-431 |
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| container_title | Biosensors & bioelectronics |
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| creator | La Belle, Jeffrey T. Bhavsar, Kinjal Fairchild, Aaron Das, Anusuya Sweeney, James Alford, T.L. Wang, Joseph Bhavanandan, Veer P. Joshi, Lokesh |
| description | A biosensor for the serum cytokine, interleukin-12 (IL-12), based upon a label-free electrochemical impedance spectroscopy monitoring is described. Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coated silver ribbon electrode by immobilizing anti-IL-12 monoclonal antibodies (mAbs) onto the surface of the electrode. This technique was advantageous as the silver electrodes provided a more rigid and conductive substrate than thin gold foil electrodes and helped in obtaining more reproducible data when used with the electrode holder. Results indicate that IL-12 can be detected at physiological levels, |
| doi_str_mv | 10.1016/j.bios.2007.07.013 |
| format | Article |
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Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coated silver ribbon electrode by immobilizing anti-IL-12 monoclonal antibodies (mAbs) onto the surface of the electrode. This technique was advantageous as the silver electrodes provided a more rigid and conductive substrate than thin gold foil electrodes and helped in obtaining more reproducible data when used with the electrode holder. Results indicate that IL-12 can be detected at physiological levels, <100fM with p<0.05 in a label-free and real-time manner. The cost-effective approach described here can be used for diagnosis of diseases (like MS) with known biomarkers in body fluids and for monitoring physiological levels of biomolecules with healthcare, food, and environmental relevance.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2007.07.013</identifier><identifier>PMID: 17851067</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biomarkers ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Disease diagnosis ; Electric Impedance ; Electrochemistry - methods ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoassay - methods ; Impedance ; Interleukin-12 - blood ; Interleukin-12 - immunology ; Interleukins ; Label-free ; Methods. Procedures. Technologies ; Multiple Sclerosis - diagnosis ; Multiple Sclerosis - immunology ; Spectrum Analysis ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2007-10, Vol.23 (3), p.428-431</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-5ca1bca4c9b4751edb061b591c85f543f5fbde2ae5896ccbd9487c0404cb5fb43</citedby><cites>FETCH-LOGICAL-c415t-5ca1bca4c9b4751edb061b591c85f543f5fbde2ae5896ccbd9487c0404cb5fb43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2007.07.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19988426$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17851067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>La Belle, Jeffrey T.</creatorcontrib><creatorcontrib>Bhavsar, Kinjal</creatorcontrib><creatorcontrib>Fairchild, Aaron</creatorcontrib><creatorcontrib>Das, Anusuya</creatorcontrib><creatorcontrib>Sweeney, James</creatorcontrib><creatorcontrib>Alford, T.L.</creatorcontrib><creatorcontrib>Wang, Joseph</creatorcontrib><creatorcontrib>Bhavanandan, Veer P.</creatorcontrib><creatorcontrib>Joshi, Lokesh</creatorcontrib><title>A cytokine immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance spectroscopy</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A biosensor for the serum cytokine, interleukin-12 (IL-12), based upon a label-free electrochemical impedance spectroscopy monitoring is described. Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coated silver ribbon electrode by immobilizing anti-IL-12 monoclonal antibodies (mAbs) onto the surface of the electrode. This technique was advantageous as the silver electrodes provided a more rigid and conductive substrate than thin gold foil electrodes and helped in obtaining more reproducible data when used with the electrode holder. Results indicate that IL-12 can be detected at physiological levels, <100fM with p<0.05 in a label-free and real-time manner. The cost-effective approach described here can be used for diagnosis of diseases (like MS) with known biomarkers in body fluids and for monitoring physiological levels of biomolecules with healthcare, food, and environmental relevance.</description><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Disease diagnosis</subject><subject>Electric Impedance</subject><subject>Electrochemistry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Impedance</subject><subject>Interleukin-12 - blood</subject><subject>Interleukin-12 - immunology</subject><subject>Interleukins</subject><subject>Label-free</subject><subject>Methods. Procedures. Technologies</subject><subject>Multiple Sclerosis - diagnosis</subject><subject>Multiple Sclerosis - immunology</subject><subject>Spectrum Analysis</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVoSTZp_0APxZfm5q1kS7INvYSQNoVAL-1ZSOMx1Va2XI1d2H8fubuQWwsjJJhvHqP3GHsn-F5woT8e9s5H2lecN_utRH3BdqJt6lJWtXrFdrxTulRa11fsmujAMyg6fsmuRNMqwXWzY-tdAccl_vITFn4c1ykSThRTMeQzrmHxc8CCIGCK5KnocUFYfJwKZwn7Yp3zM1iHoRwSYoEht1OEnzh6sCFrztjbCbLG_LdDEOfjG_Z6sIHw7fm-YT8-P3y_fyyfvn35en_3VIIUaikVWOHASuicbJTA3nEtnOoEtGpQsh7U4HqsLKq20wCu72TbAJdcgsstWd-w25PunOLvFWkxoyfAEOyEcSWj21ppIcR_wYrnhXSlMlidQMhfoYSDmZMfbToawc2WijmYLRWzpWK2EnUeen9WX92I_cvIOYYMfDgDlrJrQ8qOeXrhuq5tZaUz9-nEYTbtj8dkCDxmd3ufsrumj_5fezwDUKWunw</recordid><startdate>20071031</startdate><enddate>20071031</enddate><creator>La Belle, Jeffrey T.</creator><creator>Bhavsar, Kinjal</creator><creator>Fairchild, Aaron</creator><creator>Das, Anusuya</creator><creator>Sweeney, James</creator><creator>Alford, T.L.</creator><creator>Wang, Joseph</creator><creator>Bhavanandan, Veer P.</creator><creator>Joshi, Lokesh</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071031</creationdate><title>A cytokine immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance spectroscopy</title><author>La Belle, Jeffrey T. ; Bhavsar, Kinjal ; Fairchild, Aaron ; Das, Anusuya ; Sweeney, James ; Alford, T.L. ; Wang, Joseph ; Bhavanandan, Veer P. ; Joshi, Lokesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-5ca1bca4c9b4751edb061b591c85f543f5fbde2ae5896ccbd9487c0404cb5fb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Disease diagnosis</topic><topic>Electric Impedance</topic><topic>Electrochemistry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Impedance</topic><topic>Interleukin-12 - blood</topic><topic>Interleukin-12 - immunology</topic><topic>Interleukins</topic><topic>Label-free</topic><topic>Methods. Procedures. 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Overexpression of IL-12 has been correlated to the diagnosis of multiple sclerosis (MS). The prototype biosensor was fabricated on a disposable gold-coated silver ribbon electrode by immobilizing anti-IL-12 monoclonal antibodies (mAbs) onto the surface of the electrode. This technique was advantageous as the silver electrodes provided a more rigid and conductive substrate than thin gold foil electrodes and helped in obtaining more reproducible data when used with the electrode holder. Results indicate that IL-12 can be detected at physiological levels, <100fM with p<0.05 in a label-free and real-time manner. 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| subjects | Biological and medical sciences Biomarkers Biosensing Techniques - methods Biosensors Biotechnology Disease diagnosis Electric Impedance Electrochemistry - methods Fundamental and applied biological sciences. Psychology Humans Immunoassay - methods Impedance Interleukin-12 - blood Interleukin-12 - immunology Interleukins Label-free Methods. Procedures. Technologies Multiple Sclerosis - diagnosis Multiple Sclerosis - immunology Spectrum Analysis Various methods and equipments |
| title | A cytokine immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance spectroscopy |
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