Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast
Saccharomyces cerevisiae 424A (LNH-ST) strain was used for fermentation of glucose and xylose. Growth kinetics and ethanol productivity were calculated for batch fermentation on media containing different combinations of glucose and xylose to give a final sugar concentration of 20 ± 0.8 g/L. Growth...
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| Veröffentlicht in: | Bioresource technology 2007-02, Vol.98 (3), p.677-685 |
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| creator | Govindaswamy, Shekar Vane, Leland M. |
| description | Saccharomyces cerevisiae 424A (LNH-ST) strain was used for fermentation of glucose and xylose. Growth kinetics and ethanol productivity were calculated for batch fermentation on media containing different combinations of glucose and xylose to give a final sugar concentration of 20
±
0.8
g/L. Growth rates obtained in pure xylose-based medium were less than those for media containing pure glucose and glucose–xylose mixtures. A maximum specific growth rate
μ
max of 0.291
h
−1 was obtained in YPD medium containing 20
g/L glucose as compared to 0.206
h
−1 in YPX medium containing 20
g/L xylose. In media containing combinations of glucose and xylose, glucose was exhausted first followed by xylose. Ethanol production on pure xylose entered log phase during the 12–24
h period as compared to the 4–10
h for pure glucose based medium using 2% inoculum. When glucose was added to fermentation flasks which had been initiated on a pure xylose-based medium, the rate of xylose usage was reduced indicating cosubstrate inhibition of xylose consumption by glucose. |
| doi_str_mv | 10.1016/j.biortech.2006.02.012 |
| format | Article |
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±
0.8
g/L. Growth rates obtained in pure xylose-based medium were less than those for media containing pure glucose and glucose–xylose mixtures. A maximum specific growth rate
μ
max of 0.291
h
−1 was obtained in YPD medium containing 20
g/L glucose as compared to 0.206
h
−1 in YPX medium containing 20
g/L xylose. In media containing combinations of glucose and xylose, glucose was exhausted first followed by xylose. Ethanol production on pure xylose entered log phase during the 12–24
h period as compared to the 4–10
h for pure glucose based medium using 2% inoculum. When glucose was added to fermentation flasks which had been initiated on a pure xylose-based medium, the rate of xylose usage was reduced indicating cosubstrate inhibition of xylose consumption by glucose.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2006.02.012</identifier><identifier>PMID: 16563746</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Biotechnology ; Carbon - metabolism ; Ethanol - metabolism ; Ethanol production kinetics ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Genetic Engineering ; Genetic technics ; Glucose - metabolism ; Kinetics ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; MSF ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Substrate Specificity ; Xylose - metabolism ; YPD medium ; YPXD medium</subject><ispartof>Bioresource technology, 2007-02, Vol.98 (3), p.677-685</ispartof><rights>2006 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-5846f69b9728208f0388a9a0e73f9d15f80ffef86a23d356674fe25e829389cb3</citedby><cites>FETCH-LOGICAL-c493t-5846f69b9728208f0388a9a0e73f9d15f80ffef86a23d356674fe25e829389cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2006.02.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18457273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16563746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Govindaswamy, Shekar</creatorcontrib><creatorcontrib>Vane, Leland M.</creatorcontrib><title>Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>Saccharomyces cerevisiae 424A (LNH-ST) strain was used for fermentation of glucose and xylose. Growth kinetics and ethanol productivity were calculated for batch fermentation on media containing different combinations of glucose and xylose to give a final sugar concentration of 20
±
0.8
g/L. Growth rates obtained in pure xylose-based medium were less than those for media containing pure glucose and glucose–xylose mixtures. A maximum specific growth rate
μ
max of 0.291
h
−1 was obtained in YPD medium containing 20
g/L glucose as compared to 0.206
h
−1 in YPX medium containing 20
g/L xylose. In media containing combinations of glucose and xylose, glucose was exhausted first followed by xylose. Ethanol production on pure xylose entered log phase during the 12–24
h period as compared to the 4–10
h for pure glucose based medium using 2% inoculum. When glucose was added to fermentation flasks which had been initiated on a pure xylose-based medium, the rate of xylose usage was reduced indicating cosubstrate inhibition of xylose consumption by glucose.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Carbon - metabolism</subject><subject>Ethanol - metabolism</subject><subject>Ethanol production kinetics</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Engineering</subject><subject>Genetic technics</subject><subject>Glucose - metabolism</subject><subject>Kinetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>MSF</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Substrate Specificity</subject><subject>Xylose - metabolism</subject><subject>YPD medium</subject><subject>YPXD medium</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9vFCEUxYmxsWv1KzS86NuM_Jlh4E3TaDU26Uv7TBjmsstmFiow1n3wu8t21_SxCQkJ-Z3DvecgdElJSwkVn7bt6GMqYDctI0S0hLWEsldoReXAG6YG8RqtiBKkkT3rztHbnLeEEE4H9gadU9ELPnRihf7-9AGKtxlHh9cpPpYNNmHCUDYmxBk_pDgttvgYcD2Tdw4ShIKtSWN9yMuYSzIFMl6yD2u8hic7M897DGFdzSs_4T_7OWZoqnhX1QdwDyaXd-jMmTnD-9N9ge6_fb27-t7c3F7_uPpy09hO8dL0shNOqFENTDIiHeFSGmUIDNypifZOkjqXk8IwPvFeiKFzwHqQTHGp7Mgv0Mejb13n1wK56J3PFubZBIhL1kLyvpOEvggywrqap6qgOII2xZwTOP2Q_M6kvaZEHxrSW_2_IX1oSBOma0NVeHn6YRl3MD3LTpVU4MMJMLnm6JIJ1udnTnb9wAZeuc9HDmpwvz0kna2HYGHyCWzRU_QvzfIPXPq1EA</recordid><startdate>20070201</startdate><enddate>20070201</enddate><creator>Govindaswamy, Shekar</creator><creator>Vane, Leland M.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070201</creationdate><title>Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast</title><author>Govindaswamy, Shekar ; Vane, Leland M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-5846f69b9728208f0388a9a0e73f9d15f80ffef86a23d356674fe25e829389cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Carbon - metabolism</topic><topic>Ethanol - metabolism</topic><topic>Ethanol production kinetics</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Engineering</topic><topic>Genetic technics</topic><topic>Glucose - metabolism</topic><topic>Kinetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>MSF</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Substrate Specificity</topic><topic>Xylose - metabolism</topic><topic>YPD medium</topic><topic>YPXD medium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Govindaswamy, Shekar</creatorcontrib><creatorcontrib>Vane, Leland M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Govindaswamy, Shekar</au><au>Vane, Leland M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2007-02-01</date><risdate>2007</risdate><volume>98</volume><issue>3</issue><spage>677</spage><epage>685</epage><pages>677-685</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>Saccharomyces cerevisiae 424A (LNH-ST) strain was used for fermentation of glucose and xylose. Growth kinetics and ethanol productivity were calculated for batch fermentation on media containing different combinations of glucose and xylose to give a final sugar concentration of 20
±
0.8
g/L. Growth rates obtained in pure xylose-based medium were less than those for media containing pure glucose and glucose–xylose mixtures. A maximum specific growth rate
μ
max of 0.291
h
−1 was obtained in YPD medium containing 20
g/L glucose as compared to 0.206
h
−1 in YPX medium containing 20
g/L xylose. In media containing combinations of glucose and xylose, glucose was exhausted first followed by xylose. Ethanol production on pure xylose entered log phase during the 12–24
h period as compared to the 4–10
h for pure glucose based medium using 2% inoculum. When glucose was added to fermentation flasks which had been initiated on a pure xylose-based medium, the rate of xylose usage was reduced indicating cosubstrate inhibition of xylose consumption by glucose.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16563746</pmid><doi>10.1016/j.biortech.2006.02.012</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Bioresource technology, 2007-02, Vol.98 (3), p.677-685 |
| issn | 0960-8524 1873-2976 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biotechnology Carbon - metabolism Ethanol - metabolism Ethanol production kinetics Fermentation Fundamental and applied biological sciences. Psychology Genetic Engineering Genetic technics Glucose - metabolism Kinetics Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology MSF Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Substrate Specificity Xylose - metabolism YPD medium YPXD medium |
| title | Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast |
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