Development of an engineering autologous palatal mucosa-like tissue for potential clinical applications
The goal of this study was to optimize key processes in recreating functional and viable palatal mucosa‐like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structu...
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| Veröffentlicht in: | Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2007-11, Vol.83B (2), p.554-561 |
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| container_title | Journal of biomedical materials research. Part B, Applied biomaterials |
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| creator | Luitaud, C. Laflamme, C. Semlali, A. Saidi, S. Grenier, G. Zakrzewski, A. Rouabhia, M. |
| description | The goal of this study was to optimize key processes in recreating functional and viable palatal mucosa‐like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6‐day culture, using 105 fibroblasts and 106 epithelial cells, a well‐organized palatal mucosa‐like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16‐positive cells located in the supra‐basal layer. This palatal mucosa‐like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft‐tissue defects secondary to trauma, congenital defects, and acquired diseases. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007 |
| doi_str_mv | 10.1002/jbm.b.30828 |
| format | Article |
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Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6‐day culture, using 105 fibroblasts and 106 epithelial cells, a well‐organized palatal mucosa‐like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16‐positive cells located in the supra‐basal layer. This palatal mucosa‐like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft‐tissue defects secondary to trauma, congenital defects, and acquired diseases. © 2007 Wiley Periodicals, Inc. 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Part B, Applied biomaterials</title><addtitle>J. Biomed. Mater. Res</addtitle><description>The goal of this study was to optimize key processes in recreating functional and viable palatal mucosa‐like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6‐day culture, using 105 fibroblasts and 106 epithelial cells, a well‐organized palatal mucosa‐like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16‐positive cells located in the supra‐basal layer. This palatal mucosa‐like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft‐tissue defects secondary to trauma, congenital defects, and acquired diseases. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007</description><subject>Biocompatible Materials</subject><subject>Collagen - chemistry</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - physiology</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Gingiva - cytology</subject><subject>Humans</subject><subject>Membranes, Artificial</subject><subject>Mouth Mucosa - chemistry</subject><subject>Mouth Mucosa - cytology</subject><subject>Mouth Mucosa - growth & development</subject><subject>palatal mucosa</subject><subject>Palate - cytology</subject><subject>polymer scaffold</subject><subject>reconstruction</subject><subject>tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1552-4973</issn><issn>1552-4981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1v1TAUhq0KRD9g6o48sVS5HH_GGWlLW1CBBQSbZTvOlVsnTuOEtv--LvdSNjqdd3jeRzp6EToksCIA9P2V7Vd2xUBRtYP2iBC04o0iL55yzXbRfs5XBZYg2Cu0S2ouBVC2h9an_rePaez9MOPUYTNgP6zD4P0UhjU2y5xiWqcl49FEM5uI-8WlbKoYrj2eQ86Lx12a8JjmoggFcDEMwZVgxjGWMIc05NfoZWdi9m-29wD9OPv4_eSiuvx2_unkw2XlOG1UZVpqGgBZm9pwZRl03BFaU6l423KqqFDgJOlAWeFBKGsNd1aRtmUNkxLYAXq38Y5Tull8nnUfsvMxmsGXL7RUTHAO_FmQAQFJ1fNGCoLUDSEFPNqAbko5T77T4xR6M91rAvpxKV2W0lb_WarQb7faxfa-_cdupykA2QC3Ifr7_7n05-Mvf6XVphPy7O-eOma61rJmtdA_v55rOPtF1DFnWrEHxDqt1A</recordid><startdate>200711</startdate><enddate>200711</enddate><creator>Luitaud, C.</creator><creator>Laflamme, C.</creator><creator>Semlali, A.</creator><creator>Saidi, S.</creator><creator>Grenier, G.</creator><creator>Zakrzewski, A.</creator><creator>Rouabhia, M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200711</creationdate><title>Development of an engineering autologous palatal mucosa-like tissue for potential clinical applications</title><author>Luitaud, C. ; Laflamme, C. ; Semlali, A. ; Saidi, S. ; Grenier, G. ; Zakrzewski, A. ; Rouabhia, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4298-ad2a90067a7a48b30f4c1272684dd4282580c61f08b5e058bba4cb81dd3936603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biocompatible Materials</topic><topic>Collagen - chemistry</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - physiology</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - physiology</topic><topic>Gingiva - cytology</topic><topic>Humans</topic><topic>Membranes, Artificial</topic><topic>Mouth Mucosa - chemistry</topic><topic>Mouth Mucosa - cytology</topic><topic>Mouth Mucosa - growth & development</topic><topic>palatal mucosa</topic><topic>Palate - cytology</topic><topic>polymer scaffold</topic><topic>reconstruction</topic><topic>tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luitaud, C.</creatorcontrib><creatorcontrib>Laflamme, C.</creatorcontrib><creatorcontrib>Semlali, A.</creatorcontrib><creatorcontrib>Saidi, S.</creatorcontrib><creatorcontrib>Grenier, G.</creatorcontrib><creatorcontrib>Zakrzewski, A.</creatorcontrib><creatorcontrib>Rouabhia, M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. 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Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6‐day culture, using 105 fibroblasts and 106 epithelial cells, a well‐organized palatal mucosa‐like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16‐positive cells located in the supra‐basal layer. This palatal mucosa‐like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft‐tissue defects secondary to trauma, congenital defects, and acquired diseases. © 2007 Wiley Periodicals, Inc. 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| ispartof | Journal of biomedical materials research. Part B, Applied biomaterials, 2007-11, Vol.83B (2), p.554-561 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biocompatible Materials Collagen - chemistry Epithelial Cells - cytology Epithelial Cells - physiology Fibroblasts - cytology Fibroblasts - physiology Gingiva - cytology Humans Membranes, Artificial Mouth Mucosa - chemistry Mouth Mucosa - cytology Mouth Mucosa - growth & development palatal mucosa Palate - cytology polymer scaffold reconstruction tissue engineering Tissue Engineering - methods |
| title | Development of an engineering autologous palatal mucosa-like tissue for potential clinical applications |
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