Use of multidimensional separation protocols for the purification of trace components in complex biological samples for proteomics analysis

The routine detection of low abundance components in complex samples for detailed proteomics analysis continues to be a challenge. Whilst the potential of multidimensional chromatographic fractionation for this purpose has been proposed for some years, and was used effectively for the purification t...

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Veröffentlicht in:	Journal of Chromatography A 2007-10, Vol.1168 (1), p.190-210
Hauptverfasser:	Nice, E.C., Rothacker, J., Weinstock, J., Lim, L., Catimel, B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity MS Analytical, structural and metabolic biochemistry Biological and medical sciences Biomarkers Biosensors Chromatography, Affinity - methods Chromatography, High Pressure Liquid - methods Chromatography, Ion Exchange - methods Chromatography, Liquid - methods Electrophoresis, Gel, Two-Dimensional - methods Fundamental and applied biological sciences. Psychology General aspects, investigation methods Isoelectric Focusing - methods Multidimensional HPLC Proteins Proteomics Proteomics - instrumentation Proteomics - methods
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