A comparative study of immobilization techniques for urease on glass-pH-electrode and its application in urea detection in blood serum
Different techniques have been used (physical adsorption, physically entrapped sandwich and microencapsulation) for the immobilization of urease enzyme in tetramethylorthosilicate (TMOS) derived sol–gel matrix on the sensing surface of glass-pH-electrode. No significant leaching of enzyme occurs fro...
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| Veröffentlicht in: | Analytica chimica acta 2006-09, Vol.578 (2), p.156-161 |
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| container_title | Analytica chimica acta |
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| creator | Sahney, Rachana Anand, S. Puri, B.K. Srivastava, A.K. |
| description | Different techniques have been used (physical adsorption, physically entrapped sandwich and microencapsulation) for the immobilization of urease enzyme in tetramethylorthosilicate (TMOS) derived sol–gel matrix on the sensing surface of glass-pH-electrode. No significant leaching of enzyme occurs from the microencapsulated and physically entrapped enzyme sandwich films. Potentiometric techniques have been used for the estimation of urea concentration in each instance. Various parameters of biosensor performance have been studied which indicates that microencapsulation technique is a better method of enzyme immobilization in sol–gel films derived from TMOS. The advantage of microencapsulated biosensor over others include higher sensitivity (dpH/dp(C)
=
2.4), lower detection limit of 52
μg
mL
−1, larger linear range (0.01–30
mM) of urea determination and reasonably long-term stability of about 25 days with 80% response signal. |
| doi_str_mv | 10.1016/j.aca.2006.06.063 |
| format | Article |
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=
2.4), lower detection limit of 52
μg
mL
−1, larger linear range (0.01–30
mM) of urea determination and reasonably long-term stability of about 25 days with 80% response signal.</description><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Electrochemical methods</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microencapsulation</topic><topic>pH-electrode</topic><topic>Physical adsorption</topic><topic>Physically entrapped sandwich</topic><topic>Sol–gel technique</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahney, Rachana</creatorcontrib><creatorcontrib>Anand, S.</creatorcontrib><creatorcontrib>Puri, B.K.</creatorcontrib><creatorcontrib>Srivastava, A.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sahney, Rachana</au><au>Anand, S.</au><au>Puri, B.K.</au><au>Srivastava, A.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparative study of immobilization techniques for urease on glass-pH-electrode and its application in urea detection in blood serum</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2006-09-25</date><risdate>2006</risdate><volume>578</volume><issue>2</issue><spage>156</spage><epage>161</epage><pages>156-161</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>Different techniques have been used (physical adsorption, physically entrapped sandwich and microencapsulation) for the immobilization of urease enzyme in tetramethylorthosilicate (TMOS) derived sol–gel matrix on the sensing surface of glass-pH-electrode. No significant leaching of enzyme occurs from the microencapsulated and physically entrapped enzyme sandwich films. Potentiometric techniques have been used for the estimation of urea concentration in each instance. Various parameters of biosensor performance have been studied which indicates that microencapsulation technique is a better method of enzyme immobilization in sol–gel films derived from TMOS. The advantage of microencapsulated biosensor over others include higher sensitivity (dpH/dp(C)
=
2.4), lower detection limit of 52
μg
mL
−1, larger linear range (0.01–30
mM) of urea determination and reasonably long-term stability of about 25 days with 80% response signal.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17723707</pmid><doi>10.1016/j.aca.2006.06.063</doi><tpages>6</tpages></addata></record> |
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| subjects | Analytical chemistry Biological and medical sciences Biosensors Biotechnology Chemistry Electrochemical methods Exact sciences and technology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microencapsulation pH-electrode Physical adsorption Physically entrapped sandwich Sol–gel technique Various methods and equipments |
| title | A comparative study of immobilization techniques for urease on glass-pH-electrode and its application in urea detection in blood serum |
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