Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae
n − 3 Polyunsaturated fatty acids are assumed to play an important role in the prevention and treatment of atherosclerosis. Endothelial nitric-oxide synthase (eNOS) is responsible for cardiovascular homeostasis involving in regulation of vascular function, and the subcellular localization is critica...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 2007-10, Vol.466 (2), p.250-259 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Li, Qiurong Zhang, Qiang Wang, Meng Liu, Fuzhong Zhao, Sumin Ma, Jian Luo, Nan Li, Ning Li, Yousheng Xu, Guowang Li, Jieshou |
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3 Polyunsaturated fatty acids are assumed to play an important role in the prevention and treatment of atherosclerosis. Endothelial nitric-oxide synthase (eNOS) is responsible for cardiovascular homeostasis involving in regulation of vascular function, and the subcellular localization is critical for its activation. Here we determined the effect of docosahexaenoic acid (DHA, 22:6
n
−
3) on distribution of eNOS and its activity. DHA treatment markedly altered lipid environment of caveolae microdomains, which was coincided with selective displacement of caveolin-1 and eNOS from caveolae. Akt was not detected in caveolae fractions and CaM was distributed in both of caveolin-1-enriched membranes and non-caveolar fractions, whose distribution was unaffected by DHA. These data demonstrated for the first time that DHA altered caveolae microenvironment not only by modifying membrane lipid composition, but also by changing distribution of major structural proteins. DHA-induced alterations in caveolae lipid/protein environment may be an important mechanism in the development of pathogenesis of atherosclerosis. |
| doi_str_mv | 10.1016/j.abb.2007.06.023 |
| format | Article |
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−
3 Polyunsaturated fatty acids are assumed to play an important role in the prevention and treatment of atherosclerosis. Endothelial nitric-oxide synthase (eNOS) is responsible for cardiovascular homeostasis involving in regulation of vascular function, and the subcellular localization is critical for its activation. Here we determined the effect of docosahexaenoic acid (DHA, 22:6
n
−
3) on distribution of eNOS and its activity. DHA treatment markedly altered lipid environment of caveolae microdomains, which was coincided with selective displacement of caveolin-1 and eNOS from caveolae. Akt was not detected in caveolae fractions and CaM was distributed in both of caveolin-1-enriched membranes and non-caveolar fractions, whose distribution was unaffected by DHA. These data demonstrated for the first time that DHA altered caveolae microenvironment not only by modifying membrane lipid composition, but also by changing distribution of major structural proteins. DHA-induced alterations in caveolae lipid/protein environment may be an important mechanism in the development of pathogenesis of atherosclerosis.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/j.abb.2007.06.023</identifier><identifier>PMID: 17662956</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Calmodulin - metabolism ; Caveolae ; Caveolae - enzymology ; Caveolin 1 - metabolism ; Cell Membrane - metabolism ; Cells, Cultured ; Docosahexaenoic Acids - pharmacology ; Endothelial Cells - metabolism ; Endothelium, Vascular - cytology ; eNOS ; Fatty acid composition ; Fatty Acids - metabolism ; Humans ; Nitric Oxide Synthase Type III - metabolism ; Phospholipids ; Phospholipids - metabolism ; Polyunsaturated fatty acids ; Proto-Oncogene Proteins c-akt - metabolism</subject><ispartof>Archives of biochemistry and biophysics, 2007-10, Vol.466 (2), p.250-259</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-5e73cfc12d1ed9c91e48e0a54621ad4e031bec041c28c191caf02f0f70136a53</citedby><cites>FETCH-LOGICAL-c417t-5e73cfc12d1ed9c91e48e0a54621ad4e031bec041c28c191caf02f0f70136a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.abb.2007.06.023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17662956$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Qiurong</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Wang, Meng</creatorcontrib><creatorcontrib>Liu, Fuzhong</creatorcontrib><creatorcontrib>Zhao, Sumin</creatorcontrib><creatorcontrib>Ma, Jian</creatorcontrib><creatorcontrib>Luo, Nan</creatorcontrib><creatorcontrib>Li, Ning</creatorcontrib><creatorcontrib>Li, Yousheng</creatorcontrib><creatorcontrib>Xu, Guowang</creatorcontrib><creatorcontrib>Li, Jieshou</creatorcontrib><title>Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>n
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3 Polyunsaturated fatty acids are assumed to play an important role in the prevention and treatment of atherosclerosis. Endothelial nitric-oxide synthase (eNOS) is responsible for cardiovascular homeostasis involving in regulation of vascular function, and the subcellular localization is critical for its activation. Here we determined the effect of docosahexaenoic acid (DHA, 22:6
n
−
3) on distribution of eNOS and its activity. DHA treatment markedly altered lipid environment of caveolae microdomains, which was coincided with selective displacement of caveolin-1 and eNOS from caveolae. Akt was not detected in caveolae fractions and CaM was distributed in both of caveolin-1-enriched membranes and non-caveolar fractions, whose distribution was unaffected by DHA. These data demonstrated for the first time that DHA altered caveolae microenvironment not only by modifying membrane lipid composition, but also by changing distribution of major structural proteins. DHA-induced alterations in caveolae lipid/protein environment may be an important mechanism in the development of pathogenesis of atherosclerosis.</description><subject>Calmodulin - metabolism</subject><subject>Caveolae</subject><subject>Caveolae - enzymology</subject><subject>Caveolin 1 - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Cells, Cultured</subject><subject>Docosahexaenoic Acids - pharmacology</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium, Vascular - cytology</subject><subject>eNOS</subject><subject>Fatty acid composition</subject><subject>Fatty Acids - metabolism</subject><subject>Humans</subject><subject>Nitric Oxide Synthase Type III - metabolism</subject><subject>Phospholipids</subject><subject>Phospholipids - metabolism</subject><subject>Polyunsaturated fatty acids</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhi0EglL4ASwoE1vCXZw4iZgQ3wiJpbvlni-qqzSGOK3Kv8dVK7Ex3XDP--ruEeIKIUNAdbvMzHye5QBVBiqDXB6JCUKjUpB1cSwmACDTplZ4Js5DWAIgFio_FWdYKZU3pZqI90dPPpgFbw333lFiyNnEtC3TGBLurR8X3DnTJb0bh7j3W2c5CT_9uDCBE9cnZDbsO8MX4qQ1XeDLw5yK2fPT7OE1_fh8eXu4_0ipwGpMS64ktYS5RbYNNchFzWDKeBkaWzBInDNBgZTXhA2SaSFvoa0ApTKlnIqbfe3X4L_XHEa9coG460zPfh20qqVUqoYI4h6kwYcwcKu_Brcyw49G0Dt_eqmjP73zp0Hp6C9mrg_l6_mK7V_iICwCd3uA44cbx4MO5Lgntm6IyrT17p_6XzWSgL8</recordid><startdate>20071015</startdate><enddate>20071015</enddate><creator>Li, Qiurong</creator><creator>Zhang, Qiang</creator><creator>Wang, Meng</creator><creator>Liu, Fuzhong</creator><creator>Zhao, Sumin</creator><creator>Ma, Jian</creator><creator>Luo, Nan</creator><creator>Li, Ning</creator><creator>Li, Yousheng</creator><creator>Xu, Guowang</creator><creator>Li, Jieshou</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20071015</creationdate><title>Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae</title><author>Li, Qiurong ; Zhang, Qiang ; Wang, Meng ; Liu, Fuzhong ; Zhao, Sumin ; Ma, Jian ; Luo, Nan ; Li, Ning ; Li, Yousheng ; Xu, Guowang ; Li, Jieshou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-5e73cfc12d1ed9c91e48e0a54621ad4e031bec041c28c191caf02f0f70136a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Calmodulin - metabolism</topic><topic>Caveolae</topic><topic>Caveolae - enzymology</topic><topic>Caveolin 1 - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Cells, Cultured</topic><topic>Docosahexaenoic Acids - pharmacology</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium, Vascular - cytology</topic><topic>eNOS</topic><topic>Fatty acid composition</topic><topic>Fatty Acids - metabolism</topic><topic>Humans</topic><topic>Nitric Oxide Synthase Type III - metabolism</topic><topic>Phospholipids</topic><topic>Phospholipids - metabolism</topic><topic>Polyunsaturated fatty acids</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Qiurong</creatorcontrib><creatorcontrib>Zhang, Qiang</creatorcontrib><creatorcontrib>Wang, Meng</creatorcontrib><creatorcontrib>Liu, Fuzhong</creatorcontrib><creatorcontrib>Zhao, Sumin</creatorcontrib><creatorcontrib>Ma, Jian</creatorcontrib><creatorcontrib>Luo, Nan</creatorcontrib><creatorcontrib>Li, Ning</creatorcontrib><creatorcontrib>Li, Yousheng</creatorcontrib><creatorcontrib>Xu, Guowang</creatorcontrib><creatorcontrib>Li, Jieshou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Qiurong</au><au>Zhang, Qiang</au><au>Wang, Meng</au><au>Liu, Fuzhong</au><au>Zhao, Sumin</au><au>Ma, Jian</au><au>Luo, Nan</au><au>Li, Ning</au><au>Li, Yousheng</au><au>Xu, Guowang</au><au>Li, Jieshou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>2007-10-15</date><risdate>2007</risdate><volume>466</volume><issue>2</issue><spage>250</spage><epage>259</epage><pages>250-259</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>n
−
3 Polyunsaturated fatty acids are assumed to play an important role in the prevention and treatment of atherosclerosis. Endothelial nitric-oxide synthase (eNOS) is responsible for cardiovascular homeostasis involving in regulation of vascular function, and the subcellular localization is critical for its activation. Here we determined the effect of docosahexaenoic acid (DHA, 22:6
n
−
3) on distribution of eNOS and its activity. DHA treatment markedly altered lipid environment of caveolae microdomains, which was coincided with selective displacement of caveolin-1 and eNOS from caveolae. Akt was not detected in caveolae fractions and CaM was distributed in both of caveolin-1-enriched membranes and non-caveolar fractions, whose distribution was unaffected by DHA. These data demonstrated for the first time that DHA altered caveolae microenvironment not only by modifying membrane lipid composition, but also by changing distribution of major structural proteins. DHA-induced alterations in caveolae lipid/protein environment may be an important mechanism in the development of pathogenesis of atherosclerosis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17662956</pmid><doi>10.1016/j.abb.2007.06.023</doi><tpages>10</tpages></addata></record> |
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| subjects | Calmodulin - metabolism Caveolae Caveolae - enzymology Caveolin 1 - metabolism Cell Membrane - metabolism Cells, Cultured Docosahexaenoic Acids - pharmacology Endothelial Cells - metabolism Endothelium, Vascular - cytology eNOS Fatty acid composition Fatty Acids - metabolism Humans Nitric Oxide Synthase Type III - metabolism Phospholipids Phospholipids - metabolism Polyunsaturated fatty acids Proto-Oncogene Proteins c-akt - metabolism |
| title | Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae |
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