Detection of Mycobacterium avium subsp. paratuberculosis in ovine faeces by direct quantitative PCR has similar or greater sensitivity compared to radiometric culture

The aims of this study were to develop a new real-time quantitative PCR (QPCR) assay based on IS 900 for detection and quantification of Mycobacterium avium subsp. paratuberculosis (MAP) DNA in faeces, and to use this to detect infected sheep. Both the C and S strains of MAP were detected by the QPC...

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Veröffentlicht in:	Veterinary microbiology 2007-11, Vol.125 (1), p.36-48
Hauptverfasser:	Kawaji, Satoko, Taylor, Deborah L., Mori, Yasuyuki, Whittington, Richard J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriology Biological and medical sciences Culture Diagnosis disease detection DNA Transposable Elements - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Faeces fecal culture feces Feces - microbiology Fundamental and applied biological sciences. Psychology microbial detection Microbiology Miscellaneous Mycobacterium avium Mycobacterium avium subsp. paratuberculosis Mycobacterium avium subsp. paratuberculosis - genetics Mycobacterium avium subsp. paratuberculosis - isolation & purification Paratuberculosis Paratuberculosis - diagnosis Paratuberculosis - microbiology polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary quantitative analysis Quantitative PCR radiometry Radiometry - methods Radiometry - veterinary Sensitivity and Specificity Sheep Sheep Diseases - diagnosis Sheep Diseases - microbiology Statistics, Nonparametric strains
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