ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos

This protocol describes a method to detect in vivo associations between proteins and DNA in developing Drosophila embryos. It combines formaldehyde crosslinking and immunoprecipitation of protein-bound sequences with genome-wide analysis using microarrays. After crosslinking, nuclei are enriched usi...

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