PCR detection and typing of human papilloma virus DNA in squamous carcinoma of the cervix in a cohort of Sri Lankan women
To determine the prevalence of human papilloma virus (HPV) types 16 and 18 in squamous carcinomas of the cervix in Sri Lanka. Case control study. One gynaecological unit at the Cancer Institute, Maharagama, Sri Lanka. 15 patients with squamous carcinoma of the cervix, and 15 age matched controls wit...
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| Veröffentlicht in: | Ceylon medical journal 2009-10, Vol.51 (3), p.114-117 |
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| container_title | Ceylon medical journal |
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| creator | de Silva, Rajiva Karunaratne, K Mendis, Lalitha N Ramesh, R Chow, V T K |
| description | To determine the prevalence of human papilloma virus (HPV) types 16 and 18 in squamous carcinomas of the cervix in Sri Lanka.
Case control study.
One gynaecological unit at the Cancer Institute, Maharagama, Sri Lanka.
15 patients with squamous carcinoma of the cervix, and 15 age matched controls with histologically normal cervices.
DNA was extracted from paraffin embedded cervical biopsies. Polymerase chain reaction was performed on extracted DNA employing primers specific for HPV types 16 and 18.
HPV 16 DNA was detected in 11 out of 15 cervical cancer biopsies (73.3%), in comparison with 3 out of 15 normal controls (20%). HPV 18 was detected in 3 out of 15 cervical cancer biopsies, but not in a single control biopsy.
Despite the limited number of cases in this cohort, this study supports the strong association between HPV 16 and squamous cancer of the cervix. |
| doi_str_mv | 10.4038/cmj.v51i3.1255 |
| format | Article |
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Case control study.
One gynaecological unit at the Cancer Institute, Maharagama, Sri Lanka.
15 patients with squamous carcinoma of the cervix, and 15 age matched controls with histologically normal cervices.
DNA was extracted from paraffin embedded cervical biopsies. Polymerase chain reaction was performed on extracted DNA employing primers specific for HPV types 16 and 18.
HPV 16 DNA was detected in 11 out of 15 cervical cancer biopsies (73.3%), in comparison with 3 out of 15 normal controls (20%). HPV 18 was detected in 3 out of 15 cervical cancer biopsies, but not in a single control biopsy.
Despite the limited number of cases in this cohort, this study supports the strong association between HPV 16 and squamous cancer of the cervix.</description><identifier>ISSN: 0009-0875</identifier><identifier>EISSN: 2386-1274</identifier><identifier>DOI: 10.4038/cmj.v51i3.1255</identifier><identifier>PMID: 17315590</identifier><language>eng</language><publisher>Sri Lanka</publisher><subject>Biopsy ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - virology ; Case-Control Studies ; DNA, Viral - analysis ; Female ; Human papillomavirus 16 - genetics ; Human papillomavirus 16 - isolation & purification ; Human papillomavirus 18 - genetics ; Human papillomavirus 18 - isolation & purification ; Humans ; Papillomavirus Infections - pathology ; Papillomavirus Infections - virology ; Polymerase Chain Reaction ; Risk Factors ; Sri Lanka ; Uterine Cervical Neoplasms - pathology ; Uterine Cervical Neoplasms - virology</subject><ispartof>Ceylon medical journal, 2009-10, Vol.51 (3), p.114-117</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c274t-5d3971b8cabea9875cb68bee6815890ec3682dbc7e413fdac2bad2965704651b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17315590$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Silva, Rajiva</creatorcontrib><creatorcontrib>Karunaratne, K</creatorcontrib><creatorcontrib>Mendis, Lalitha N</creatorcontrib><creatorcontrib>Ramesh, R</creatorcontrib><creatorcontrib>Chow, V T K</creatorcontrib><title>PCR detection and typing of human papilloma virus DNA in squamous carcinoma of the cervix in a cohort of Sri Lankan women</title><title>Ceylon medical journal</title><addtitle>Ceylon Med J</addtitle><description>To determine the prevalence of human papilloma virus (HPV) types 16 and 18 in squamous carcinomas of the cervix in Sri Lanka.
Case control study.
One gynaecological unit at the Cancer Institute, Maharagama, Sri Lanka.
15 patients with squamous carcinoma of the cervix, and 15 age matched controls with histologically normal cervices.
DNA was extracted from paraffin embedded cervical biopsies. Polymerase chain reaction was performed on extracted DNA employing primers specific for HPV types 16 and 18.
HPV 16 DNA was detected in 11 out of 15 cervical cancer biopsies (73.3%), in comparison with 3 out of 15 normal controls (20%). HPV 18 was detected in 3 out of 15 cervical cancer biopsies, but not in a single control biopsy.
Despite the limited number of cases in this cohort, this study supports the strong association between HPV 16 and squamous cancer of the cervix.</description><subject>Biopsy</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - virology</subject><subject>Case-Control Studies</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Human papillomavirus 16 - genetics</subject><subject>Human papillomavirus 16 - isolation & purification</subject><subject>Human papillomavirus 18 - genetics</subject><subject>Human papillomavirus 18 - isolation & purification</subject><subject>Humans</subject><subject>Papillomavirus Infections - pathology</subject><subject>Papillomavirus Infections - virology</subject><subject>Polymerase Chain Reaction</subject><subject>Risk Factors</subject><subject>Sri Lanka</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Uterine Cervical Neoplasms - virology</subject><issn>0009-0875</issn><issn>2386-1274</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtPwzAQhC0EoqVw5Yh84pZgx3HiHKvylCpAPM6R7TjUJbGDnRT673FoJU6r1c6Mdj4AzjGKU0TYlWzX8YZiTWKcUHoApglhWYSTPD0EU4RQESGW0wk48X6NggGh_BhMcE4wpQWagu3z4gVWqley19ZAbirYbzttPqCt4WpouYEd73TT2JbDjXaDh9ePc6gN9F8Db23YJXdSm_EeLP1KQancRv-MGg6lXVnXj5dXp-GSm8-Q-G1bZU7BUc0br872cwbeb2_eFvfR8unuYTFfRjKU6CNakSLHgkkuFC9CFykyJpTKGKasQEqSjCWVkLlKMakrLhPBq6TIaI7SjGJBZuByl9s5-zUo35et9lI1DTcqvF9mjOACExaE8U4onfXeqbrsnG6525YYlSPsMsAu_2CXI-xguNgnD6JV1b98T5f8AkXlfHk</recordid><startdate>20091029</startdate><enddate>20091029</enddate><creator>de Silva, Rajiva</creator><creator>Karunaratne, K</creator><creator>Mendis, Lalitha N</creator><creator>Ramesh, R</creator><creator>Chow, V T K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091029</creationdate><title>PCR detection and typing of human papilloma virus DNA in squamous carcinoma of the cervix in a cohort of Sri Lankan women</title><author>de Silva, Rajiva ; Karunaratne, K ; Mendis, Lalitha N ; Ramesh, R ; Chow, V T K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c274t-5d3971b8cabea9875cb68bee6815890ec3682dbc7e413fdac2bad2965704651b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biopsy</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - virology</topic><topic>Case-Control Studies</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Human papillomavirus 16 - genetics</topic><topic>Human papillomavirus 16 - isolation & purification</topic><topic>Human papillomavirus 18 - genetics</topic><topic>Human papillomavirus 18 - isolation & purification</topic><topic>Humans</topic><topic>Papillomavirus Infections - pathology</topic><topic>Papillomavirus Infections - virology</topic><topic>Polymerase Chain Reaction</topic><topic>Risk Factors</topic><topic>Sri Lanka</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Uterine Cervical Neoplasms - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Silva, Rajiva</creatorcontrib><creatorcontrib>Karunaratne, K</creatorcontrib><creatorcontrib>Mendis, Lalitha N</creatorcontrib><creatorcontrib>Ramesh, R</creatorcontrib><creatorcontrib>Chow, V T K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Ceylon medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Silva, Rajiva</au><au>Karunaratne, K</au><au>Mendis, Lalitha N</au><au>Ramesh, R</au><au>Chow, V T K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR detection and typing of human papilloma virus DNA in squamous carcinoma of the cervix in a cohort of Sri Lankan women</atitle><jtitle>Ceylon medical journal</jtitle><addtitle>Ceylon Med J</addtitle><date>2009-10-29</date><risdate>2009</risdate><volume>51</volume><issue>3</issue><spage>114</spage><epage>117</epage><pages>114-117</pages><issn>0009-0875</issn><eissn>2386-1274</eissn><abstract>To determine the prevalence of human papilloma virus (HPV) types 16 and 18 in squamous carcinomas of the cervix in Sri Lanka.
Case control study.
One gynaecological unit at the Cancer Institute, Maharagama, Sri Lanka.
15 patients with squamous carcinoma of the cervix, and 15 age matched controls with histologically normal cervices.
DNA was extracted from paraffin embedded cervical biopsies. Polymerase chain reaction was performed on extracted DNA employing primers specific for HPV types 16 and 18.
HPV 16 DNA was detected in 11 out of 15 cervical cancer biopsies (73.3%), in comparison with 3 out of 15 normal controls (20%). HPV 18 was detected in 3 out of 15 cervical cancer biopsies, but not in a single control biopsy.
Despite the limited number of cases in this cohort, this study supports the strong association between HPV 16 and squamous cancer of the cervix.</abstract><cop>Sri Lanka</cop><pmid>17315590</pmid><doi>10.4038/cmj.v51i3.1255</doi><tpages>4</tpages></addata></record> |
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| ispartof | Ceylon medical journal, 2009-10, Vol.51 (3), p.114-117 |
| issn | 0009-0875 2386-1274 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biopsy Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - virology Case-Control Studies DNA, Viral - analysis Female Human papillomavirus 16 - genetics Human papillomavirus 16 - isolation & purification Human papillomavirus 18 - genetics Human papillomavirus 18 - isolation & purification Humans Papillomavirus Infections - pathology Papillomavirus Infections - virology Polymerase Chain Reaction Risk Factors Sri Lanka Uterine Cervical Neoplasms - pathology Uterine Cervical Neoplasms - virology |
| title | PCR detection and typing of human papilloma virus DNA in squamous carcinoma of the cervix in a cohort of Sri Lankan women |
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