use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms
This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteri...
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Veröffentlicht in: | Letters in applied microbiology 2007-10, Vol.45 (4), p.364-370 |
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creator | Parry, J.D Holmes, A.K Unwin, M.E Laybourn-Parry, J |
description | This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling. |
doi_str_mv | 10.1111/j.1472-765X.2007.02213.x |
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Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.2007.02213.x</identifier><identifier>PMID: 17897377</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Acanthamoeba castellanii ; Amoeba ; Animals ; Bacteria ; Bacterial Physiological Phenomena ; Biofilm ; Biofilms ; Biological and medical sciences ; Bodo designis ; Eukaryota - physiology ; Fundamental and applied biological sciences. Psychology ; Klebsiella aerogenes ; Microbiology ; microcolonies ; Protozoa ; Pseudomonas aeruginosa ; Tetrahymena pyriformis ; topography ; ultrasonic ; Ultrasonography - methods</subject><ispartof>Letters in applied microbiology, 2007-10, Vol.45 (4), p.364-370</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4523-153571fea3ffb3423f05de3e93998a21f04da3d7a9b226235d55bf608870d5653</citedby><cites>FETCH-LOGICAL-c4523-153571fea3ffb3423f05de3e93998a21f04da3d7a9b226235d55bf608870d5653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2007.02213.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2007.02213.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19113440$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17897377$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parry, J.D</creatorcontrib><creatorcontrib>Holmes, A.K</creatorcontrib><creatorcontrib>Unwin, M.E</creatorcontrib><creatorcontrib>Laybourn-Parry, J</creatorcontrib><title>use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.</description><subject>Acanthamoeba castellanii</subject><subject>Amoeba</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacterial Physiological Phenomena</subject><subject>Biofilm</subject><subject>Biofilms</subject><subject>Biological and medical sciences</subject><subject>Bodo designis</subject><subject>Eukaryota - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Klebsiella aerogenes</subject><subject>Microbiology</subject><subject>microcolonies</subject><subject>Protozoa</subject><subject>Pseudomonas aeruginosa</subject><subject>Tetrahymena pyriformis</subject><subject>topography</subject><subject>ultrasonic</subject><subject>Ultrasonography - methods</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhi0EotvCK4AvcEsY23HsHDhUFRSkRRygEjdrktjbrJJ4sZO2u0-P013RI_hiS_P9npE_E0IZ5CytD9ucFYpnqpS_cg6gcuCcifzhGVn9LTwnK-BlmWkuizNyHuMWADTj1UtyxpSulFBqRQ5ztNQ7OvdTwOjHrqHdgJtu3NDJU3uH_YyTpdOtpdY520wLvAt-8gePI90EPCwsji0d_J0d7JiI8ZGf_M6n-u52v2RqbCYbOuxp3XnX9UN8RV447KN9fdovyM3nTz-vvmTr79dfry7XWVNILjImhVTMWRTO1aLgwoFsrbCVqCqNnDkoWhStwqrmvORCtlLWrgStFbSylOKCvD_em8b-Pds4maGLje17HK2foym1YLoq9D9BDkoIVagE6iPYBB9jsM7sQnq1sDcMzCLIbM3iwSwezCLIPAoyDyn65tRjrgfbPgVPRhLw7gRgbLB3Acemi09cxZgoCkjcxyN33_V2_98DmPXlt-WU8m-PeYfe4CakHjc_ODCxfBIABuIPPZK2bQ</recordid><startdate>200710</startdate><enddate>200710</enddate><creator>Parry, J.D</creator><creator>Holmes, A.K</creator><creator>Unwin, M.E</creator><creator>Laybourn-Parry, J</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200710</creationdate><title>use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms</title><author>Parry, J.D ; Holmes, A.K ; Unwin, M.E ; Laybourn-Parry, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4523-153571fea3ffb3423f05de3e93998a21f04da3d7a9b226235d55bf608870d5653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acanthamoeba castellanii</topic><topic>Amoeba</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacterial Physiological Phenomena</topic><topic>Biofilm</topic><topic>Biofilms</topic><topic>Biological and medical sciences</topic><topic>Bodo designis</topic><topic>Eukaryota - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Klebsiella aerogenes</topic><topic>Microbiology</topic><topic>microcolonies</topic><topic>Protozoa</topic><topic>Pseudomonas aeruginosa</topic><topic>Tetrahymena pyriformis</topic><topic>topography</topic><topic>ultrasonic</topic><topic>Ultrasonography - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parry, J.D</creatorcontrib><creatorcontrib>Holmes, A.K</creatorcontrib><creatorcontrib>Unwin, M.E</creatorcontrib><creatorcontrib>Laybourn-Parry, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parry, J.D</au><au>Holmes, A.K</au><au>Unwin, M.E</au><au>Laybourn-Parry, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2007-10</date><risdate>2007</risdate><volume>45</volume><issue>4</issue><spage>364</spage><epage>370</epage><pages>364-370</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. Coupons of dialysis membrane were incubated in Chalkley's medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities. The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy. Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17897377</pmid><doi>10.1111/j.1472-765X.2007.02213.x</doi><tpages>7</tpages></addata></record> |
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subjects | Acanthamoeba castellanii Amoeba Animals Bacteria Bacterial Physiological Phenomena Biofilm Biofilms Biological and medical sciences Bodo designis Eukaryota - physiology Fundamental and applied biological sciences. Psychology Klebsiella aerogenes Microbiology microcolonies Protozoa Pseudomonas aeruginosa Tetrahymena pyriformis topography ultrasonic Ultrasonography - methods |
title | use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms |
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