Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry

High-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometry was developed and applied to the proteome analysis of bronchoalveolar lavage fluid (BALF) from a patient with pulmonary alveolar proteinosis. With use of 1-D and 2-D gel electrophoresis, surfactant protein A (SP-A) a...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2007-10, Vol.389 (4), p.1075-1085
Hauptverfasser:	Bai, Yu, Galetskiy, Dmitry, Damoc, Eugen, Ripper, Jan, Woischnik, Markus, Griese, Matthias, Liu, Zhiqiang, Liu, Shuying, Przybylski, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Bronchoalveolar lavage fluid Bronchoalveolar Lavage Fluid - chemistry Cyclotrons Electrophoresis, Gel, Two-Dimensional - methods Fourier Analysis Fourier transform ion cyclotron resonance mass spectrometry Hemoglobins - analysis Hemoglobins - metabolism Humans Leukocyte L1 Antigen Complex - analysis Leukocyte L1 Antigen Complex - metabolism Lung surfactant proteins Molecular Sequence Data Proline - metabolism Protein Isoforms - analysis Protein Isoforms - metabolism Protein Processing, Post-Translational Proteome - analysis Proteome - metabolism Proteomics - methods Pulmonary alveolar proteinosis Pulmonary Alveolar Proteinosis - metabolism Pulmonary Alveoli - metabolism Pulmonary Surfactant-Associated Protein A - analysis Pulmonary Surfactant-Associated Protein A - metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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