Protein synthesis in liposomes with a minimal set of enzymes

In a significant step towards the construction of the semi-synthetic minimal cell, a protein expression system with a minimal set of pure and specific enzymes is required. A novel cell-free transcription and translation system named PURESYSTEM (PS), consisting of a specified set of 36 enzymes and ri...

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Veröffentlicht in:	Biochemical and biophysical research communications 2007-11, Vol.363 (1), p.12-17
Hauptverfasser:	Murtas, Giovanni, Kuruma, Yutetsu, Bianchini, Paolo, Diaspro, Alberto, Luisi, Pier Luigi
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomimetics - methods Cell-Free System Cell-free transcription and translation Enzyme Activation Escherichia coli - enzymology Escherichia coli Proteins - chemistry Liposomes Liposomes - chemistry Minimal cell Minimal genome Multienzyme Complexes - chemistry Origin of life Protein synthesis Proteins - chemical synthesis Synthetic biology
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