A Novel Agar Medium to Detect Hydrogen Peroxide-Producing Bacteria Based on the Prussian Blue-Forming Reaction
The classic method for H2O2 detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H2O2 produced by bacteria. Using this medium, colonies of H2O2‐producing species including Streptococcus pyogenes were easily identified by th...
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Veröffentlicht in: | Microbiology and immunology 2007-01, Vol.51 (9), p.889-892 |
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creator | Saito, Mitsumasa Seki, Masanori Iida, Ken-ichiro Nakayama, Hiroaki Yoshida, Shin-ichi |
description | The classic method for H2O2 detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H2O2 produced by bacteria. Using this medium, colonies of H2O2‐producing species including Streptococcus pyogenes were easily identified by the appearance of blue halos. The utility of the medium was further illustrated by its successful application to the isolation of H2O2‐producing mutants from a non‐H2O2‐producing stain of S. pyogenes. |
doi_str_mv | 10.1111/j.1348-0421.2007.tb03971.x |
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source | MEDLINE; Wiley Journals; Open Access Titles of Japan; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
subjects | Agar agar medium Bacteriological Techniques Chromogenic Compounds Culture Media H2O2 Humans hydrogen peroxide Hydrogen Peroxide - analysis Hydrogen Peroxide - metabolism prussian blue Prussian Blue Reaction - methods Streptococcal Infections - microbiology Streptococcus pyogenes Streptococcus pyogenes - growth & development Streptococcus pyogenes - isolation & purification Streptococcus pyogenes - metabolism |
title | A Novel Agar Medium to Detect Hydrogen Peroxide-Producing Bacteria Based on the Prussian Blue-Forming Reaction |
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