A Novel Agar Medium to Detect Hydrogen Peroxide-Producing Bacteria Based on the Prussian Blue-Forming Reaction

The classic method for H2O2 detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H2O2 produced by bacteria. Using this medium, colonies of H2O2‐producing species including Streptococcus pyogenes were easily identified by th...

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Veröffentlicht in:Microbiology and immunology 2007-01, Vol.51 (9), p.889-892
Hauptverfasser: Saito, Mitsumasa, Seki, Masanori, Iida, Ken-ichiro, Nakayama, Hiroaki, Yoshida, Shin-ichi
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container_issue 9
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container_title Microbiology and immunology
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creator Saito, Mitsumasa
Seki, Masanori
Iida, Ken-ichiro
Nakayama, Hiroaki
Yoshida, Shin-ichi
description The classic method for H2O2 detection involving Prussian blue formation was adapted to formulate a novel agar medium that makes possible in situ detection of H2O2 produced by bacteria. Using this medium, colonies of H2O2‐producing species including Streptococcus pyogenes were easily identified by the appearance of blue halos. The utility of the medium was further illustrated by its successful application to the isolation of H2O2‐producing mutants from a non‐H2O2‐producing stain of S. pyogenes.
doi_str_mv 10.1111/j.1348-0421.2007.tb03971.x
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source MEDLINE; Wiley Journals; Open Access Titles of Japan; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection
subjects Agar
agar medium
Bacteriological Techniques
Chromogenic Compounds
Culture Media
H2O2
Humans
hydrogen peroxide
Hydrogen Peroxide - analysis
Hydrogen Peroxide - metabolism
prussian blue
Prussian Blue Reaction - methods
Streptococcal Infections - microbiology
Streptococcus pyogenes
Streptococcus pyogenes - growth & development
Streptococcus pyogenes - isolation & purification
Streptococcus pyogenes - metabolism
title A Novel Agar Medium to Detect Hydrogen Peroxide-Producing Bacteria Based on the Prussian Blue-Forming Reaction
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